Sunday, May 04, 2025

Texas Senate Bill 2651 establishment of a pilot program to breed deer resistant to CWD TSE Prion

Texas Senate Bill 2651 establishment of a pilot program to breed deer resistant to CWD TSE Prion
Bill Text: TX SB2651 | 2025-2026 | 89th Legislature | Introduced
Texas Senate Bill 2651
TX State Legislature page for SB2651
Summary Sponsors Texts Votes Research Comments Track Introduced Bill Title:
Relating to the regulation of chronic wasting disease and the establishment of a pilot program to breed deer resistant to chronic wasting disease.
Spectrum: Partisan Bill (Republican 1-0)
Status: (Introduced) 2025-04-03 - Referred to Water, Agriculture, & Rural Affairs [SB2651 Detail]
Download: Texas-2025-SB2651-Introduced.html 89R14878 MP-F By: Hall S.B. No. 2651 A BILL TO BE ENTITLED AN ACT relating to the regulation of chronic wasting disease and the establishment of a pilot program to breed deer resistant to chronic wasting disease.
BE IT ENACTED BY THE LEGISLATURE OF THE STATE OF TEXAS:
SECTION 1. Chapter 12, Parks and Wildlife Code, is amended by adding Subchapter I to read as follows:
SUBCHAPTER I. CHRONIC WASTING DISEASE
Sec. 12.801. DEFINITIONS. In this subchapter:
(1) "Deer breeder facility" means a breeder facility regulated under Subchapter L, Chapter 43, or rules adopted under that subchapter.
(2) "Release site" means a location as described by Section 43.361.
Sec. 12.802. IDENTIFICATION OF CHRONIC WASTING DISEASE.
The diagnosis of chronic wasting disease in a deer under this subchapter must be made or confirmed through testing:
(1) conducted by the National Veterinary Services Laboratories; and
(2) within the National Veterinary Services Laboratories' standard time for diagnosis after the National Veterinary Services Laboratories receives the specimen.
Sec. 12.803. RESTRICTING MOVEMENT OF DEER AT DEER BREEDER FACILITY OR RELEASE SITE.
(a) Subject to Subsection (b), the department may order a restriction on the movement of deer at a deer breeder facility or release site if there is a confirmed diagnosis of chronic wasting disease at the facility or release site.
(b) The department may only restrict the movement of deer in an area that is not greater than 10 feet from any point on the original enclosure that housed the deer with a confirmed diagnosis of chronic wasting disease.
(c) The department shall lift a movement restriction ordered under this section if:
(1) the owner or manager of the deer breeder facility or release site provides a letter to the department certifying that the facility or release site has:
(A) avoided commingling any deer that have a confirmed diagnosis of chronic wasting disease or any deer that were located in an enclosure with the diagnosed deer, including:
(i) direct contact with the diagnosed deer;
(ii) being within 10 feet of the diagnosed deer; or
(iii) sharing equipment, pasture, water sources, or other contaminated locations at the facility or release site with the diagnosed deer;
(B) followed United States Department of Agriculture procedures for decontamination of farmed cervid facilities, including decontamination of all enclosures, surfaces, tools, equipment, vehicles, clothing, surgical instruments, or any other items that have come in contact or commingled with a deer that has a confirmed diagnosis of chronic wasting disease; and
(C) depopulated the enclosure of all deer; and
(2) there is no confirmed diagnosis of chronic wasting disease of a free-ranging deer that conclusively establishes that the free-ranging deer populations near the deer breeder facility or release site have been infected with chronic wasting disease due to the wilful and deliberate failure of the owner or manager of the deer breeder facility or release site to adhere to the criteria of this subsection.
(d) If the department fails to lift a movement restriction ordered under this section as required by Subsection (c), the owner or manager of the deer breeder facility or release site may appeal to the commission. If the commission fails to lift the movement restriction, the owner or manager of the deer breeder facility or release site may bring an action against the department for damages resulting from the failure to lift the movement restriction and appropriate equitable relief.
(e) The department may not require the owner or manager of a deer breeder facility or release site to enter into a herd plan for the facility or release site if the herd plan is in any way more restrictive than:
(1) the provisions of Subsection (c); or
(2) the minimum standards of a herd plan developed under the Chronic Wasting Disease Herd Certification Program established by the United States Department of Agriculture.
(f) Governmental immunity to suit and liability is waived to the extent of liability created by Subsection (d).
Sec. 12.804. TESTING NEAR AREA UNDER RESTRICTED MOVEMENT ORDER. If the department requires testing of deer at a deer breeder facility or release site near an area placed under a restricted movement order under Section 12.803, the department may require a postmortem and live animal testing requirement of a number of animals that does not exceed 80 percent of the total number of animals that die each year at the facility. Any live tests submitted under the testing requirement must be accounted for at a ratio of 1:1 to postmortem tests.
Sec. 12.805. CHRONIC WASTING DISEASE GENETIC IMPROVEMENT PILOT PROGRAM.
(a) The department shall establish a pilot program to breed and release into the wild deer that are resistant to chronic wasting disease.
(b) The pilot program established under this section must include:
(1) the collection and analysis of DNA samples in native free range white-tailed deer to determine a baseline of genetic codon markers and genomic breeding values;
(2) the breeding of native white-tailed deer, born and raised in this state, to be genetically resistant to chronic wasting disease, including the SS allele at codon 96; and
(3) the monitoring for chronic wasting disease in all deer released under Subsection (c) or sold under Subsection (d).
(c) Except as provided by Subsection (d), the department may only release deer into the wild under the program between February 1 and April 15 of each year.
(d) The department may sell for a fee not to exceed $500 deer bred under the program to a person holding a valid deer breeder's permit under Section 43.352.
(e) This section expires December 31, 2029.
SECTION 2. As soon as practicable after the effective date of this Act, the Parks and Wildlife Commission shall adopt or amend rules as necessary to comply with the changes in law made by this Act.
SECTION 3. This Act takes effect September 1, 2025.
LEGISLATING CWD TSE Prion, Bills to release Genetically Modified Cervid into the wild, what could go wrong?
“If slower disease progression results in longer-lived, infected deer with longer periods of infectiousness, resistance may lead to increased disease transmission rates, higher prion concentrations in the environment, and increased prevalence, as has been observed in some captive deer herds (Miller et al., 2006; Keane et al., 2008a).”
Genetic susceptibility to chronic wasting disease in free-ranging white-tailed deer: Complement component C1q and Prnp polymorphisms§
Julie A. Blanchong a, *, Dennis M. Heisey b , Kim T. Scribner c , Scot V. Libants d , Chad Johnson e , Judd M. Aiken e , Julia A. Langenberg f , Michael D. Samuel g
snip…
Identifying the genetic basis for heterogeneity in disease susceptibility or progression can improve our understanding of individual variation in disease susceptibility in both free-ranging and captive populations. What this individual variation in disease susceptibility means for the trajectory of disease in a population, however, is not straightforward. For example, the greater, but not complete, resistance to CWD in deer with at least one Serine (S) at amino acid 96 of the Prnp gene appears to be associated with slower progression of disease (e.g., Johnson et al., 2006; Keane et al., 2008a). If slower disease progression results in longer-lived, infected deer with longer periods of infectiousness, resistance may lead to increased disease transmission rates, higher prion concentrations in the environment, and increased prevalence, as has been observed in some captive deer herds (Miller et al., 2006; Keane et al., 2008a). Alternatively, if the slower progression of disease in resistant deer is not associated with longer periods of infectiousness, but might instead indicate a higher dose of PrPCWD is required for infection, transmission rates in the population could decline especially if, as in Wisconsin, deer suffer high rates of mortality from other sources (e.g., hunting). Clearly, determining the relationship between genetic susceptibility to infection, dose requirements, disease progression, and the period of PrPCWD infectiousness are key components for understanding the consequences of CWD to free-ranging populations.
TEXAS CWD, WHILE IGNORING THE PROBLEM AT HAND, TRYING TO GMO YOUR WAY OUT SAID PROBLEM, TRYING TO LEGISLATING CWD, AND IN DOING SO, POTENTIALLY CREATING A BIGGER PROBLEM, WHAT IF?
WE call this TSE Prion Poker, are you all?
Volume 30, Number 10—October 2024
Research
Temporal Characterization of Prion Shedding in Secreta of White-Tailed Deer in Longitudinal Study of Chronic Wasting Disease, United States
Our findings suggest that deer expressing alternative PRNP polymorphisms might live longer and, although they shed fewer prions throughout CWD course, might over their extended lifespan increase CWD prions in the environment
Prion protein gene sequence and chronic wasting disease susceptibility in white-tailed deer (Odocoileus virginianus)
Adam L Brandt, Amy C Kelly, Michelle L Green, Paul Shelton, Jan Novakofski & Nohra E Mateus-Pinilla
Pages 449-462 | Received 21 Sep 2015, Accepted 23 Oct 2015, Published online: 21 Dec 2015 https://doi.org/10.1080/19336896.2015.1115179
The presence of aa96S has been associated with slowed disease progression, longer life span among captive deer,Citation26,27 and does not appear to affect the rate at which prions are shed from infected individuals.Citation38 Additionally, CWD infected mule deer have been found to excrete pathogenic prions while asymptomatic.Citation39 This contributes to concerns that wild deer with aa96S may be shedding infectious prions into the environment for longer periods of time than deer lacking the mutation, but are not symptomatic or detectable by immunohistochemical procedures.
''There are no known familial or genetic TSEs of animals, although polymorphisms in the PRNP gene of some species (sheep for example) may influence the length of the incubation period and occurrence of disease.''
c) The commonest form of CJD occurs as a sporadic disease, the cause of which is unknown, although genetic factors (particularly the codon 129 polymorphism in the prion protein gene (PRNP)) influence disease susceptibility. The familial forms of human TSEs (see Box 1) appear to have a solely genetic origin and are closely associated with mutations or insertions in the PRNP gene. Most, but not all, of the familial forms of human TSEs have been transmitted experimentally to animals. There are no known familial or genetic TSEs of animals, although polymorphisms in the PRNP gene of some species (sheep for example) may influence the length of the incubation period and occurrence of disease.
P-145 Estimating chronic wasting disease resistance in cervids using real time quaking- induced conversion
Nicholas J Haley1, Rachel Rielinqer2, Kristen A Davenport3, W. David Walter4, Katherine I O'Rourke5, Gordon Mitchell6, Juergen A Richt2 1
Our studies demonstrate that in vitro amplification metrics predict in vivo susceptibility, and that alleles with multiple codons, each influencing resistance independently, do not necessarily contribute additively to resistance. Importantly, we found that the white-tailed deer 226K substrate exhibited the slowest amplification rate among those evaluated, suggesting that further investigation of this allele and its resistance in vivo are warranted to determine if absolute resistance to CWD is possible. ***at present, no cervid PrP allele conferring absolute resistance to prion infection has been identified.
PRION 2016 CONFERENCE TOKYO
***> at present, no PrPC allele conferring absolute resistance in cervids has been identified.
J Gen Virol. 2017 Nov; 98(11): 2882–2892.
Published online 2017 Oct 23. doi: 10.1099/jgv.0.000952
Estimating chronic wasting disease susceptibility in cervids using real-time quaking-induced conversion
Chronic wasting disease (CWD) resistance in cervids is often characterized as decreased prevalence and/or protracted disease progression in individuals with specific alleles; at present, no PrPC allele conferring absolute resistance in cervids has been identified.
TEXAS BREEDER DEER ESCAPEE WITH CWD IN THE WILD, or so the genetics would show?
OH NO, please tell me i heard this wrong, a potential Texas captive escapee with cwd in the wild, in an area with positive captive cwd herd?
apparently, no ID though. tell me it ain't so please...
23:00 minute mark
''Free Ranging Deer, Dr. Deyoung looked at Genetics of this free ranging deer and what he found was, that the genetics on this deer were more similar to captive deer, than the free ranging population, but he did not see a significant connection to any one captive facility that he analyzed, so we believe, Ahhhhhh, this animal had some captive ahhh, whatnot.''
Texas symposium Cwd
Arkansas Cwd
Wyoming Cwd 2022 test results
key takeaways ;
CWD substantially reduces deer survival rates and suppresses population growth.
Where CWD prevalence is high, deer populations are likely declining. 
If CWD continues to spread, it will eventually impact deer populations elsewhere.
THE CWD TSE Prion aka mad cow type disease is not your normal pathogen.
The TSE prion disease survives ashing to 600 degrees celsius, that’s around 1112 degrees farenheit.
you cannot cook the TSE prion disease out of meat.
you can take the ash and mix it with saline and inject that ash into a mouse, and the mouse will go down with TSE.
Prion Infected Meat-and-Bone Meal Is Still Infectious after Biodiesel Production as well.
the TSE prion agent also survives Simulated Wastewater Treatment Processes.
IN fact, you should also know that the TSE Prion agent will survive in the environment for years, if not decades.
you can bury it and it will not go away.
The TSE agent is capable of infected your water table i.e. Detection of protease-resistant cervid prion protein in water from a CWD-endemic area.
it’s not your ordinary pathogen you can just cook it out and be done
New studies on the heat resistance of hamster-adapted scrapie agent: Threshold survival after ashing at 600°C suggests an inorganic template of replication
Prion Infected Meat-and-Bone Meal Is Still Infectious after Biodiesel Production
Detection of protease-resistant cervid prion protein in water from a CWD-endemic area
Prions in Waterways
A Quantitative Assessment of the Amount of Prion Diverted to Category 1 Materials and Wastewater During Processing
Rapid assessment of bovine spongiform encephalopathy prion inactivation by heat treatment in yellow grease produced in the industrial manufacturing process of meat and bone meals
THURSDAY, FEBRUARY 28, 2019
BSE infectivity survives burial for five years with only limited spread
So, this is what we leave our children and grandchildren?
Detection of chronic wasting disease prions in the farm soil of the Republic of Korea
Here, we show that prion seeding activity was detected in extracts from farm soil following 4 years of incubation with CWD-infected brain homogenate.
"Additionally, we have determined that prion seeding activity is retained for at least fifteen years at a contaminated site following attempted remediation."
Detection of prions in soils contaminated by multiple routes
Results: We are able to detect prion seeding activity at multiple types of environmental hotspots, including carcass sites, contaminated captive facilities, and scrapes (i.e. urine and saliva). Differences in relative prion concentration vary depending on the nature and source of the contamination. Additionally, we have determined that prion seeding activity is retained for at least fifteen years at a contaminated site following attempted remediation.
Conclusions: Detection of prions in the environment is of the utmost importance for controlling chronic wasting disease spread. Here, we have demonstrated a viable method for detection of prions in complex environmental matrices. However, it is quite likely that this method underestimates the total infectious prion load in a contaminated sample, due to incomplete recovery of infectious prions. Further refinements are necessary for accurate quantification of prions in such samples, and to account for the intrinsic heterogeneities found in the broader environment.
Funded by: Wisconsin Department of Natural Resources
Prion 2023 Abstracts
15 YEARS!!!
Chronic wasting disease prions on deer feeders and wildlife visitation to deer feeding areas
Miranda H. J. Huang, Steve Demarais, Marc D. Schwabenlander, Bronson K. Strickland, Kurt C. VerCauteren, William T. McKinley, Gage Rowden, Corina C. Valencia Tibbitts … See all authors
First published: 10 February 2025
Abstract
Eliminating supplemental feeding is a common regulatory action within chronic wasting disease (CWD) management zones. These regulations target the potential for increased animal-animal contact and environmental contamination with CWD prions. Prions, the causative agent of CWD, have been detected on feeder surfaces in CWD-positive, captive deer facilities but not among free-ranging populations, and information on the relative risk of transmission at anthropogenic and natural food sources is limited. In this study, we established and maintained 13 gravity feeders from September 2022 to March 2023 in a CWD zone in northern Mississippi, USA (apparent prevalence ~30%). We set up feeders up in 3 ways: no exclusion (deer feeders, n = 7), exclusion of deer using fencing with holes cut at the ground-level to permit smaller wildlife to enter (raccoon feeders, n = 3), and environmental control feeders, which were fully fenced and not filled with feed (control feeders, n = 3). We swabbed feeder spouts at setup and at 4 intervals approximately 6 weeks apart to test for prion contamination via real-time quaking-induced conversion (RT-QuIC). We detected prions 12 weeks after setup on all deer and raccoon feeders. We compared relative transmission risk using camera traps at these feeders, 6 agronomic plantings for wildlife forage (i.e., food plots), and 7 oak mast trees. Weekly visitation rate by white-tailed deer (Odocoileus virginianus; hereafter: deer) differed (P = 0.02) among deer feeders (median = 24.5 deer/week, range = 15.6–65.7), food plots (median = 12.7, range = 3.8–24.7), and mast trees (median = 2.0, range = 0.4–5.1). Contact rates between individual deer also differed between site types (P < 0.01): deer feeders (median = 2.1 deer-to-deer contacts/week, range = 0–10.1), food plots (median = 0.1, range = 0–4.0), and mast trees (median = 0, range = 0–0.3). Raccoons also visited feeders at greater rates than food plots and mast trees (P < 0.04). Finally, we swabbed 19 feeders in 2 areas where CWD was newly detected, finding prion contamination on swabs from 4 feeders. We show that deer feeders in free-ranging populations with high CWD prevalence become contaminated with CWD prions quickly, becoming a potential site of exposure of deer to CWD prions. Our results also demonstrate the ability to find evidence of prion contamination on deer feeders, even in areas where CWD is newly detected.
Snip…
We found that supplemental feeding increased the risk of exposure to CWD prions due to contamination of feeders, increased deer visitation, and increased deer-to-deer contact.
The 12-fold increase in deer visitation to feeders compared to mast trees and 2-fold increase compared to food plots demonstrates increased risk for direct disease spread.
Artificial mineral sites that pre-date endemic chronic wasting disease become prion hotspots
The detection of PrPCWD in soils at attractant sites within an endemic CWD zone significantly advances our understanding of environmental PrPCWD accumulation dynamics, providing valuable information for advancing adaptive CWD management approaches.
https://int-cwd-sympo.org/wp-content/uploads/2023/06/final-agenda-with-abstracts.pdf
Chronic wasting disease detection in environmental and biological samples from a taxidermy site
Results: The PMCA analysis demonstrated CWD seeding activity in some of the components of this facility, including insects involved in head processing, soils, and a trash dumpster.
Conclusions: Different areas of this property were used for various taxidermy procedures. We were able to detect the presence of prions in i) soils that were in contact with the heads of dead animals, ii) insects involved in the cleaning of skulls, and iii) an empty dumpster where animal carcasses were previously placed. This is the first report demonstrating that swabbing is a helpful method to screen for prion infectivity on surfaces potentially contaminated with CWD. These findings are relevant as this swabbing and amplification strategy may be used to evaluate the disease status of other free-ranging and captive settings where there is a concern for CWD transmissions, such as at feeders and water troughs with CWD-exposed properties. This approach could have substantial implications for free-ranging cervid surveillance as well as in epidemiological investigations of CWD.
Prion 2022 Conference abstracts: pushing the boundaries
***> Infectious agent of sheep scrapie may persist in the environment for at least 16 years
***> Nine of these recurrences occurred 14–21 years after culling, apparently as the result of environmental contamination, but outside entry could not always be absolutely excluded.
JOURNAL OF GENERAL VIROLOGY Volume 87, Issue 12
Infectious agent of sheep scrapie may persist in the environment for at least 16 years Free
Rapid recontamination of a farm building occurs after attempted prion removal
First published: 19 January 2019 https://doi.org/10.1136/vr.105054
The data illustrates the difficulty in decontaminating farm buildings from scrapie, and demonstrates the likely contribution of farm dust to the recontamination of these environments to levels that are capable of causing disease. 
snip...
This study clearly demonstrates the difficulty in removing scrapie infectivity from the farm environment. Practical and effective prion decontamination methods are still urgently required for decontamination of scrapie infectivity from farms that have had cases of scrapie and this is particularly relevant for scrapie positive goatherds, which currently have limited genetic resistance to scrapie within commercial breeds.24 This is very likely to have parallels with control efforts for CWD in cervids.
***>This is very likely to have parallels with control efforts for CWD in cervids.
Durkin: Wisconsin DNR says CWD sinking deer herds in disease-endemic areas
PATRICK DURKIN Outdoors Columnist
CWD culprits
According to Wisconsin DNR research, a healthy buck’s annual survival chances are 69% in southwestern Wisconsin, while a CWD-infected buck has only a 17% chance of being alive a year later. The 3-year-old Richland County buck, left, tested positive for CWD in 2020. The yearling buck skeleton couldn’t be tested for CWD, but was found in 2021 on the same infected farm.
If you’re seeing too few deer in southwestern Wisconsin for your hunting or viewing pleasure, it’s time to accept the obvious reason.
The culprit is chronic wasting disease, the always fatal sickness whose infectious prions now kill more female deer in highly contaminated areas than hunters kill with bullets and arrows. Roughly speaking, that’s much of Iowa, Sauk and Richland counties, and western Dane County.
The Department of Natural Resources confirmed that fact for the first time Jan. 22when releasing the latest findings of its long-running $5 million study into how CWD affects deer populations. The study found that once CWD infects 29% or more of an area’s female deer, the herd starts declining as more deer die each year than reproduction replaces.
As Jasmine Batten, supervisor of the DNR’s wildlife health section, emailed her staff, “CWD mortality has largely replaced antlerless harvest as the primary driver of the deer population’s trajectory in the CWD endemic area (west of Madison).”
Neither Batten nor the agency rushed to that conclusion. The DNR launched its “Southwest Wisconsin CWD, Deer and Predator Study” in autumn 2016, and then caught, tested and fitted GPS collars to 1,249 animals over the next four years across northeastern Grant County, northern Iowa County and northwestern Dane County.
The agency then monitored those 810 adult deer, 323 fawns, 69 coyotes and 47 bobcats to learn where they lived, when and where they moved, and when/how they died. When a collar signaled the animal’s death, researchers hurried in, hoping to learn what killed it.
The DNR says this ongoing study is the “largest and most comprehensive deer research project ever undertaken in Wisconsin.” Although the data will provide more findings, this fact won’t change under current hunting regulations: Other than two-legged hunters targeting bucks, CWD has no deer-killing equal once it’s widespread.
“We can now say it’s not EHD (epizootic hemorrhagic disease), it’s not coyotes, it’s not bobcats, and it’s not earn-a-buck regulations from 15 years ago that are causing the herd declines we’re seeing,” said Dan Storm, the study’s chief researcher. “CWD is the cause, and we have solid evidence to back it up. This is what’s going on, and so let’s proceed with what to do about it.”
The study found that a healthy, uninfected doe age 1 or older is twice as likely to be alive a year later than a CWD-infected doe. Specifically, a healthy doe’s annual survival chances are 83%, while an infected doe’s chances are 41%. CWD-infected bucks age 1 and older fare four times worse than healthy bucks. Specifically, a healthy buck’s annual survival chances are 69%, while an infected buck’s chances are 17%.
CWD-infected deer more often get hit by vehicles, shot by hunters, and killed by starvation and pneumonia. In fact, 51% of dead deer necropsied in the study had pneumonia. Further, preliminary summaries show end-stage wasting — which includes infections and starvation — is the No. 1 cause of death (57%) for CWD-positive adult does. Sick does more often reach that stage than sick bucks, given hunters’ focus on antlers after lawmakers eliminated earn-a-buck regulations in 2011.
So yes, contrary to endemic social-media nonsense, CWD kills deer. In fact, as the cause of death for 57% of infected does, it outpaces the next three causes: hunting, coyotes and unknown causes. For healthy, CWD-free does, hunting and vehicle collisions caused 75% of deaths. Bacterial infections, coyotes and unknown causes killed the other 25%.
Fawn survival in the study was high enough to sustain deer herds. Of the study’s 323 fawns, the annual percentage reaching age 1 ranged from 43% to 51%. Fawn survival rates across North America in recent decades range from 10% to 90%.
The study found that predators (mainly coyotes, but also bobcats) kill about 31% of the unnual fawn “crop,” while diseases like pneumonia, EHD and enterocolitis (inflamed intestines) take 6%; hunters, 4%; human-related causes (vehicles, pet dogs and haying/mowing/brush-hogging), 4%; and starvation, 3%.
Skeptics, of course, ignore CWD while blaming predators and EHD for declining herds. Though they clamor for other hunters to quit shooting antlerless deer, no legitimate deer biologist supports passivity.
“We already did that and look how it went,” Storm said. “Before we lost earn-a-buck (in 2011), hunters dropped Iowa County’s deer herd below 20,000. After earn-a-buck, the herd took 7%, 10% and 12% annual increases until 2020. That herd should have kept growing, but it didn’t. CWD is pulling it down and boxing it in.”
The DNR’s annual post-hunt population estimates show Iowa County’s herd rose 51.3% from 16,900 in 2011 to 25,566, the 2018-2020 three-year average. The herd has since fallen 15.25% to 21,666, the 2021-2023 three-year average.
Bryan Richards, CWD project leader at the USDA’s National Wildlife Health Center in Madison, said backing off would backfire. “You won’t recover a population by letting CWD run its course,” Richards said. “When you try to stockpile deer by not shooting, you protect sick deer, too. Contamination worsens and the healthy proportion of the herd declines. Shooting removes sick deer from the herd sooner than CWD will. They’ll spread fewer prions over time, and you’ll probably shoot them before CWD reaches its worst stages for shedding prions.”
Storm put it this way: “The more CWD you have in your area, the more the herd will decline.” Which areas already exceed 29% infection rates for adult does? The latest DNR data from a year ago shows southeastern Richland County on the edge at 27%, northwestern Iowa County at 35%, and the Devil’s Lake area in eastern Sauk County at 34%.
Further, CWD testing of hunter-killed deer in autumn 2024 shows overall (bucks and does) detection rates at or above 29% in six townships (6-mile by 6-mile areas) in Columbia County, three townships in Dane County, eight townships in Iowa County, 11 townships in Richland County, and 15 townships in Sauk County.
How low will deer populations drop where CWD is endemic? Storm said CWD won’t exterminate deer, but no one can predict how it will affect specific valleys, woodlands or watersheds. CWD has spread at varying rates in different Wisconsin habitats, and appears to have leveled off at high infection rates in some areas while still rising and spreading in others.
The disease has so far been verified in wild deer in 48 of Wisconsin’s 72 counties, even though testing has been totally voluntary for years. During the 2024 hunting season, 1,755 more deer tested positive for CWD across the state, a record 10.4% detection rate despite the least amount of samples (16,939) volunteered since 2017. Richland County hunters provided the most samples, 1,335, in 2024, and 444 (33.4%) had CWD.
Southwest Wisconsin CWD, Deer and Predator Study
key takeaways ;
CWD substantially reduces deer survival rates and suppresses population growth.
Where CWD prevalence is high, deer populations are likely declining. 
If CWD continues to spread, it will eventually impact deer populations elsewhere.
MONDAY, APRIL 28, 2025
Wisconsin DNR 2024 CWD 1,786 samples testing positive
The effectiveness of harvest for limiting wildlife disease: Insights from 20 years of chronic wasting disease in Wyoming
Wynne E. Moss, Justin Binfet, L. Embere Hall, Samantha E. Allen, William H. Edwards, Jessica E. Jennings-Gaines, Paul C. Cross
First published: 21 January 2025
https://doi.org/10.1002/eap.3089
Vertical transmission of chronic wasting disease in free-ranging white-tailed deer populations
Audrey M. Sandoval, Amy V. Nalls, Erin E. McNulty, Nathaniel D. Denkers, Devon J. Trujillo, Zoe Olmstead, Ethan Barton, Jennifer R. Ballard, Daniel M. Grove, Jeremy S. Dennison, Natalie Stilwell, Christopher A. Cleveland, James M. Crum, Mark G. Ruder, Candace K. Mathiason doi: https://doi.org/10.1101/2025.01.24.634834
ABSTRACT

Chronic wasting disease (CWD) is a fatal neurodegenerative disease affecting cervids across North America, Northern Europe, and Asia. Disease transmission among cervids has historically been attributed to direct animal-to-animal contact with ‘secreta’ (saliva, blood, urine, and feces) containing the infectious agent, and indirect contact with the agent shed to the environment in these bodily components. Mounting evidence provides another mechanism of CWD transmission, that from mother-to-offspring, including during pregnancy (vertical transmission). Here we describe the detection of the infectious CWD agent and prion seeding in fetal and reproductive tissues collected from healthy-appearing free-ranging white-tailed deer (Odocoileus virginianus) from multiple U.S. states by mouse bioassay and in vitro prion amplification assays. This is the first report of the infectious agent in several in utero derived fetal and maternal-fetal reproductive tissues, providing evidence that CWD infections are propagated within gestational fetal tissues of white-tailed deer populations. This work confirms previous experimental and field findings in several cervid species supporting vertical transmission as a mechanism of CWD transmission and helps to further explain the facile dissemination of this disease among captive and free-ranging cervid populations.
snip…
Overall, this study describes the dissemination of CWD prions throughout tissues and birthing fluids of the pregnancy microenvironment demonstrating that offspring are routinely exposed to the infectious prion in-utero prior to parturition.
Texas CWD Surveillance Positives Tracking Page is still outdated, last figures i have were;
Texas CWD total by calendar years
TPWD CWD Tracking
Counties where CWD Exposed Deer were Released
Number of CWD Exposed Deer Released by County
CWD Status Captive Herds
Aug 18, 2021
Oh, Deer
Heading Off a Wildlife Epidemic
CWD poses a significant threat to the future of hunting in Texas. Deer population declines of 45 and 50 percent have been documented in Colorado and Wyoming. A broad infection of Texas deer populations resulting in similar population impacts would inflict severe economic damage to rural communities and could negatively impact land markets. Specifically, those landowners seeking to establish a thriving herd of deer could avoid buying in areas with confirmed CWD infections. As they do with anthrax-susceptible properties, land brokers may find it advisable to inquire about the status of CWD infections on properties that they present for sale. Prospective buyers should also investigate the status of the wildlife on prospective properties. In addition, existing landowners should monitor developments as TPWD crafts management strategies to identify and contain this deadly disease.
Dr. Gilliland (c-gilliland@tamu.edu) is a research economist with the Texas Real Estate Research Center at Texas A&M University.
Texas Game Wardens Bust Illegal Deer Operations Across the State Feb. 27, 2025
Media Contact: TPWD News, Business Hours, 512-389-8030
AUSTIN – A recent investigation by Texas Game Wardens resulted in approximately 1,200 pending charges and 22 suspects from across the state involved in the deer breeding industry and black-market wildlife trade.
The suspects and charges are associated with three deer breeding facilities, ten release sites, one deer management pen and three illegal facilities not registered in the Texas Wildlife Information Management Services (TWIMS) database, meaning they were operating or receiving deer in violation of registration requirements and disease monitoring protocols.
“CWD spreads among wild populations at a relatively slow rate, limited by the natural home range and dispersed nature of wild animals.”
NOW HOLD YOUR HORSES, Chronic Wasting Disease CWD of Cervid can spread rather swiftly, traveling around 50 MPH, from the back of truck and trailer, and Here in Texas, we call it ‘Trucking CWD’…
Preventive Veterinary Medicine Volume 234, January 2025, 106385
Use of biosecurity practices to prevent chronic wasting disease in Minnesota cervid herds
Vehicles or trailers that entered the farm were used to transport other live cervids, cervid carcasses, or cervid body parts in past 3 years in 64.3 % (95 % CI 46.3–82.3) of larger elk/reindeer herds compared to 13.6 % (95 % CI 4.7–22.4) of smaller deer herds.
Snip…
Identifying the exact pathway of initial CWD transmission to cervid herds is often not possible, in part due to many potential pathways of transmission for the infection, including both direct and indirect contact with infected farmed or wild cervids (Kincheloe et al., 2021). That study identified that transmissions from infected farmed cervids may occur from direct contact with the movement of cervids from one herd to another and from indirect contact with the sharing of equipment, vehicles, clothing, reproductive equipment, and potentially through semen or embryos.
***> Department records indicate that within the last five years (since January 1, 2020), 30 deer breeding facilities where CWD has been confirmed transferred a total of 8,799 deer to 249 additional deer breeding facilities and 487 release sites located in a total of 144 counties in Texas. <***
Texas Kimble County Farm Chronic Wasting Disease CWD TSE Prion Approximate Herd Prevalence 12%
SUMMARY MINUTES OF THE 407th COMMISSION MEETING Texas Animal Health Commission
September 22, 2020
Chronic Wasting Disease (CWD):
A new CWD positive breeding herd was disclosed in February 2020 in Kimble County. This herd depopulation was completed in July 2020. Including the two index positive deer, an additional eight more positive deer were disclosed (approximate herd prevalence 12%). Since July 2015 and prior to this discovery, five positive captive breeder herds have been disclosed and four of those are in Medina County. One herd in Lavaca and three herds in Medina County were depopulated leaving one large herd in Medina County that is managed on a herd plan. A new zone was established in Val Verde County in December 2019 as a result of a positive free-ranging White-tailed Deer (WTD). A second positive WTD was also disclosed in February 2020 in the same area.
SUMMARY MINUTES OF THE 407th COMMISSION MEETING – 9/22/2020
Scrapie: The flock identified in April 2016 remains under quarantine in Hartley County
“It is interesting to note that, in 2001, the State of Texas shifted its deer management strategies toward the same leanings that Kroll has suggested for Wisconsin. In Texas, the change was brought about via heavy lobbying from the high-fence deer ranching industry. This pressure helped convince the Texas Parks and Wildlife to change their regulations and allow private landowners to select the own deer biologists.”
According to Wisconsin’s White-Tailed Deer Trustee Dr. James Kroll, people who call for more public hunting opportunities are “pining for socialism.” He further states, “(Public) Game management is the last bastion of communism.”
“Game Management,” says James Kroll, driving to his high-fenced, two-hundred-acre spread near Nacogdoches, “is the last bastion of communism.” Kroll, also known as Dr. Deer, is the director of the Forestry Resources Institute of Texas at Stephen F. Austin State University, and the “management” he is referring to is the sort practiced by the State of Texas. The 55-year-old Kroll is the leading light in the field of private deer management as a means to add value to the land. His belief is so absolute that some detractors refer to him as Dr. Dough, implying that his eye is on the bottom line more than on the natural world.
Kroll, who has been the foremost proponent of deer ranching in Texas for more than thirty years, doesn’t mind the controversy and certainly doesn’t fade in the heat. People who call for more public lands are “cocktail conservationists,” he says, who are really pining for socialism. He calls national parks “wildlife ghettos” and flatly accuses the government of gross mismanagement. He argues that his relatively tiny acreage, marked by eight-foot fences and posted signs warning off would-be poachers, is a better model for keeping what’s natural natural while making money off the land.
Dr. Deer Wisconsin Report: Will High-Fence Bias Skew Final Plan?
Categories: Blogs, Daniel Schmidt’s Whitetail Wisdom, Deer News, Featured Tags: antler restricitons, dan schmidt, Dr. Deer, james kroll, James Kroll Wisconsin, qdm, quality deer management, texas hunting, wisconsin deer hunting March 29, 2012 According to Wisconsin’s White-Tailed Deer Trustee Dr. James Kroll, people who call for more public hunting opportunities are “pining for socialism.” He further states, “(Public) Game management is the last bastion of communism.”
OPINION BLOG
These are just two insights into the man who has been asked to provide analysis and recommended changes to Wisconsin’s deer management program. Kroll’s insights are from an article entitled “Which Side of the Fence Are You On?” by Joe Nick Patoski for a past edition of Texas Monthly. If nothing more, the article gives an unabashed look into the mind-set that will be providing the Wisconsin DNR with recommendations on how to change their deer management practices. James Kroll (also known as “Deer Dr.”) was appointed to the Wisconsin “deer czar” position last fall. He was hired by the Department of Administration and instructed to complete a review of the state’s deer management program.
Here’s a sample of the article:
“Game Management,” says James Kroll, driving to his high-fenced, two-hundred-acre spread near Nacogdoches, “is the last bastion of communism.” Kroll, also known as Dr. Deer, is the director of the Forestry Resources Institute of Texas at Stephen F. Austin State University, and the “management” he is referring to is the sort practiced by the State of Texas. The 55-year-old Kroll is the leading light in the field of private deer management as a means to add value to the land. His belief is so absolute that some detractors refer to him as Dr. Dough, implying that his eye is on the bottom line more than on the natural world.
Kroll, who has been the foremost proponent of deer ranching in Texas for more than thirty years, doesn’t mind the controversy and certainly doesn’t fade in the heat. People who call for more public lands are “cocktail conservationists,” he says, who are really pining for socialism. He calls national parks “wildlife ghettos” and flatly accuses the government of gross mismanagement. He argues that his relatively tiny acreage, marked by eight-foot fences and posted signs warning off would-be poachers, is a better model for keeping what’s natural natural while making money off the land.
A trip to South Africa six years ago convinced Kroll that he was on the right track. There he encountered areas of primitive, lush wildlife-rich habitats called game ranches. They were privately owned, privately managed, and enclosed by high fences. He noticed how most of the land outside those fences had been grazed to the nub, used up. “Game ranches there derive their income from these animals — viewing them, hunting them, selling their meat,” he says. “There are no losers.” At his own ranch Kroll has set up a smaller version of the same thing. His land is indeed lush, verdant, with pine groves, an abundance of undergrowth, wild orchids, New Jersey tea, jack-in-the-pulpits, and other native plants. He has also set up a full-scale breeding research center and is one of twenty Texas deer breeders using artificial insemination to improve his herd. “We balance sex and age ratio,” he says. “We manage habitat. We control the population and manage for hunting. I want to leave the deer herd better than it was before we came.”
It is interesting to note that, in 2001, the State of Texas shifted its deer management strategies toward the same leanings that Kroll has suggested for Wisconsin. In Texas, the change was brought about via heavy lobbying from the high-fence deer ranching industry. This pressure helped convince the Texas Parks and Wildlife to change their regulations and allow private landowners to select the own deer biologists.
“That has given landowners more freedom,” Kroll told Texas Monthly. “(However,) You still have to let the state on your land to get a wildlife-management permit.” The key difference here is that 98 percent of Texas is comprised of private land. Wisconsin, on the other hand, consists of approximately 34.8 million acres of land, and 25.5 percent of the state’s 638,000 gun-hunters reported hunting on public land at some point during the season (2010, Duey, Rees).
According to the Wisconsin Realtors Association, more than 5.7 million acres of this land, or 16.5 percent, is publicly owned and used for parks, forests, trails, and natural resource protection. [Note: these statistics do not include the public land used for roads, government buildings, military bases, and college/school campuses.] This 5.7 million acres of public land is owned as follows:
Federal government owns approximately 1.5 million acres (4.4 percent of the state’s land area). Almost all of the federal forestland in Wisconsin is located in Chequamegon-Nicolet National Forest.
State government owns approximately 1.6 million acres (4.6 percent of the state’s land area). The land is managed by two agencies, the Board of Commissioners of Public Land (who manages lands granted by federal government) and the DNR (managing land owned by the state).
County government owns approximately 2.6 million acres (7.5 percent of the state’s land area).
Public land is located in 71 of Wisconsin’s 72 counties, with the most public land located in Bayfield County (464,673 acres). [Note: Menominee County does not have any public land, but 98 percent of the land is held in trust by the Menominee Tribe.] Twenty counties have more than 100,000 acres of public land, while only 12 counties have fewer than 10,000 acres.
What does this all mean? My initial reaction, which is one that I predicted when Kroll was named to the state’s deer trustee position, is that his team’s final recommendations — if implemented — will be heavily skewed toward the state’s larger landowners (500+ acres) and folks who own small parcels in areas comprised mostly of private land.
It is also my prediction that the final recommendations (again, if implemented) will do little, if anything, to improve deer herds and deer hunting on Wisconsin’s 5.7 million acres of public land.
Where does this leave the public-land hunter? “It will suck to be you,” said one deer manager who asked to remain anonymous out of fear for his job. “The resources and efforts will go toward improving the private land sector. This is all about turning deer hunting away from the Public Land Doctrine and more toward a European-style of management — like they have in Texas.”
I do, of course, hope these assumptions are wrong. As with all things in life, we should maintain an open mind to change. Life is all about change. However, change for the sake of change is usually a recipe for disaster. Especially when that change is driven by something more than a sincere desire to manage public resources for the greater good.
As noted yesterday (Dr. James Kroll Report: Is That All You Get For Your Money), I will provide more of my opinions and interpretation on this important issue in forthcoming installments of this blog. Read his full preliminary report here.
http://www.texasmonthly.com/preview/2002-02-01/feature5
***> Politicians, sperm mills, Semen, And CWD, what if?
Jerking for Dollars, and CWD TSE Prion, what if$$$???
Talk about big bucks: Deer semen donations are fueling South Texas campaign Each deer semen straw — from bucks with names like Gladiator Sunset, Sweet Dreams and Bandit — was assigned a $1,000 value, according to her campaign finance report.
=====
Powerful Abbott appointee's lobbying sparks blowback in Legislature
In an ironic twist for Gov. Greg Abbott, who has made ethics reform an urgent political priority, the Texas House is taking aim at what critics call a "pay to play" culture among his appointees.
Powerful Abbott appointee's lobbying sparks blowback in Legislature In an ironic twist for Gov. Greg Abbott, who has made ethics reform an urgent political priority, the Texas House is taking aim at what critics call a "pay to play" culture among his appointees.
BY JAY ROOT MAY 12, 201712 AM CENTRAL
Talk about big bucks: Deer semen donations are fueling South Texas campaign Each deer semen straw — from bucks with names like Gladiator Sunset, Sweet Dreams and Bandit — was assigned a $1,000 value, according to her campaign finance report.
Talk about big bucks: Deer semen donations are fueling South Texas campaign Each deer semen straw — from bucks with names like Gladiator Sunset, Sweet Dreams and Bandit — was assigned a $1,000 value, according to her campaign finance report.
AUSTIN — Donations of deer semen, one of Texas deer breeders’ most precious commodities, account for more than half of the contributions to a South Texan’s state House campaign.
Snip…
Each deer semen straw — from bucks with names like Gladiator Sunset, Sweet Dreams and Bandit — was assigned a $1,000 value, according to her campaign finance report. A straw refers to the container of ejaculate that is stored for later use. Breeders market their deers’ antler size and shape as reasons to buy straws from their bucks. Uvalde deer breeder Fred Gonzalez said the donors’ straws went into a semen tank to be sold as one lot at a Texas Deer Association event last month and donated to her campaign.
Gonzalez, the treasurer of the Texas Deer Association, donated one straw to the lot. He said the deer breeding community often donates straws instead of money, although not usually directly to a political campaign.
“Semen is a very common way for us to donate,” he said. “One collection on a buck could lead to 60 straws sometimes. If you have a desirable animal, it’s a way to bring value without breaking the bank.”
The Texas Deer Association’s political action committee has received $976,025 in deer semen donations between 2006 and 2016. It has given $885,695 to campaigns and interest groups in the same span. According to expenditure reports between 2006 and 2016, the PAC has never given in-kind donations in the form of deer semen. Though the straws donated to Garza were sold at a Texas Deer Association event, the organization’s political action committee did not contribute to her campaign
Texas Deer Association contributions
The association’s political action committee has contributed $885,695 to campaigns and interest groups between 2006 and 2016. These are the top 10 candidates who have received money.
Candidate Amount
Rep. Ernest Bailes (R) $45,000
Rep. Lyle Larson (R) $26,611
Rep. Lance Gooden (R) $21,250
House Speaker Joe Straus (R) $21,000
Lt. Gov. Dan Patrick (R) $20,000
Comptroller Glenn Hegar (R) $16,000
Sen. Juan Hinojosa (D) $13,500
Rep. Todd Hunter (R) $13,000
Rep. Ryan Guillen (D) $12,750
Sen. Craig Estes (R) $12,500
SOURCE: Texas Ethics Commission
Deer semen donations among campaign contributions to South Texas candidate
Donations were made as part of an auction event
By Andrea Zelinski Published 1:26 pm CST, Wednesday, February 28, 2018
A South Texas candidate for the state House reported $51,000 worth of campaign contributions in deer semen, according to campaign finance reports.
AUSTIN — Many political candidates accept political gifts like food for events or legal advice for their campaigns, but one candidate from South Texas reported receiving thousands of dollars worth of deer semen.
Ana Lisa Garza, a district court judge in Starr County, reported accepting at least 40 semen straws, doses valued at $51,000. According to a report filed with the Texas Ethics Commission, several of the in-kind donations were made as part of a Feb. 10 auction event.
Although deer have been bred for over a century, interest has spiked in recent decades, in part due to interest in a buck named Patrick that was kept as a pet in the Midwest and grew large and unique antlers in the 1980s.
The practice has since grown into a budding industry in Texas. The deer, with their attractive racks, are now largely used for hunting, venison or further breeding.
In the filings, the straws are largely named after their sperm donors, including "Mabo Thicket" "Tack Hammer," "Strike Force." Other names of the straws include, "Bambi Chewy."
The economic impact of the deer breeding industry is $349.4 million annually in the state, according to a 2017 study by Texas A&M University. Combined with hunting, the study valued the industry's economic impact at $1.6 billion annually, according to the report.
The Texas Deer Association did not respond to requests for comment.
Iatrogenic TSE Prion, Friendly Fire, Unforeseen Circumstances
Vertical transmission of chronic wasting disease in free-ranging white-tailed deer populations
Audrey M. Sandoval, Amy V. Nalls, Erin E. McNulty, Nathaniel D. Denkers, Devon J. Trujillo, Zoe Olmstead, Ethan Barton, Jennifer R. Ballard, Daniel M. Grove, Jeremy S. Dennison, Natalie Stilwell, Christopher A. Cleveland, James M. Crum, Mark G. Ruder, Candace K. Mathiason doi: Vertical transmission of chronic wasting disease in free-ranging white-tailed deer populations
ABSTRACT
Chronic wasting disease (CWD) is a fatal neurodegenerative disease affecting cervids across North America, Northern Europe, and Asia. Disease transmission among cervids has historically been attributed to direct animal-to-animal contact with ‘secreta’ (saliva, blood, urine, and feces) containing the infectious agent, and indirect contact with the agent shed to the environment in these bodily components. Mounting evidence provides another mechanism of CWD transmission, that from mother-to-offspring, including during pregnancy (vertical transmission). Here we describe the detection of the infectious CWD agent and prion seeding in fetal and reproductive tissues collected from healthy-appearing free-ranging white-tailed deer (Odocoileus virginianus) from multiple U.S. states by mouse bioassay and in vitro prion amplification assays. This is the first report of the infectious agent in several in utero derived fetal and maternal-fetal reproductive tissues, providing evidence that CWD infections are propagated within gestational fetal tissues of white-tailed deer populations. This work confirms previous experimental and field findings in several cervid species supporting vertical transmission as a mechanism of CWD transmission and helps to further explain the facile dissemination of this disease among captive and free-ranging cervid populations.
snip…
Overall, this study describes the dissemination of CWD prions throughout tissues and birthing fluids of the pregnancy microenvironment demonstrating that offspring are routinely exposed to the infectious prion in-utero prior to parturition.
Protein misfolding cyclic amplification (PMCA) as an ultra-sensitive technique for the screening of CWD prions in different sample types.
Francisca Bravo-Risi1,2, Paulina Soto1,2, Rebeca Benavente1, Hunter Reed3, Mitch Lockwood3, Tracy A. Nichols4, Rodrigo Morales1,5 1Department of Neurology, The University of Texas Health Science Center at Houston, Houston, USA. 2Universidad Bernardo O’Higgins, Doctorado en Ciencias con Mención en Materiales Funcionales, Santiago, Chile. 3Texas Park and Wildlife Department, Austin, USA. 4Veterinary Services Cervid Health Program, United States Department of Agriculture, Animal and Plant Health Inspection Service, Fort Collins, USA. 5Centro Integrativo de Biologia y Quimica Aplicada (CIBQA), Universidad Bernardo O’Higgins, Santiago, Chile
Abstract
Chronic wasting disease (CWD) is a prion disease that affects farmed and free-ranging cervids. Currently, CWD status is ultimately confirmed in the brain and lymphoid tissues by immunohistochemistry (IHC). One limitation of IHC is its relatively poor sensitivity making it difficult to detect this disease early in the incubation period which can extend 1-3 years. Protein misfolding cyclic amplification (PMCA) and real-time quaking-induced conversion (RT-QuIC) are ultra-sensitive techniques that provide a means to detect CWD in early stages of the disease. PMCA mimics the self-propagation of infectious prions in vitro through multiple incubation-sonication cycles, increasing the number of prion particles present in a given sample. The detection of proteinase K (PK)-resistant PrPSc by PMCA has been performed in experimental and natural samples that may otherwise go undetected using traditional diagnostic techniques.
In this study, we highlight recent advances and contributions that our group has made detecting CWD-prions in animal and environmental samples collected from deer breeding and taxidermy facilities. Additionally, CWD-prions were detected in samples from hunter-harvested, free-ranging animals.
PMCA successfully detected CWD-prions in a diverse array of samples including blood, semen, feces, obex, retropharyngeal lymph node, fetuses (neural and peripheral tissues) and gestational tissues, parasites-insects, plants, compost-soil mixtures, and swabs from trash containers.
Importantly, our findings identified CWD in areas previously considered to be free of CWD. Overall, our findings demonstrate that PMCA is a powerful technique for the screening of biological and environmental samples, and it may prove useful as a CWD management and surveillance tool.
RESEARCH ARTICLE
In Vitro detection of Chronic Wasting Disease (CWD) prions in semen and reproductive tissues of white tailed deer bucks (Odocoileus virginianus)
Carlos Kramm, Ruben Gomez-Gutierrez, Claudio Soto, Glenn Telling, Tracy Nichols, Rodrigo Morales
Abstract
Chronic Wasting Disease (CWD) is a prion disease affecting several cervid species. Among them, white-tailed deer (WTD) are of relevance due to their value in farming and game hunting. The exact events involved in CWD transmission in captive and wild animals are still unclear. An unexplored mechanism of CWD spread involves transmissions through germplasm, such as semen. Surprisingly, the presence and load of CWD prions in semen and male sexual tissues from WTD has not been explored. Here, we described the detection of CWD prions in semen and sexual tissues of WTD bucks utilizing the Protein Misfolding Cyclic Amplification (PMCA) technology. Samples were obtained post-mortem from farmed pre-clinical, CWD positive WTD bucks possessing polymorphisms at position 96 of the PRNP gene. Our results show that overall CWD detection in these samples had a sensitivity of 59.3%, with a specificity of 97.2%. The data indicate that the presence of CWD prions in male sexual organs and fluids is prevalent in late stage, pre-clinical, CWD-infected WTD (80%-100% of the animals depending on the sample type analyzed). Our findings reveal the presence of CWD prions in semen and sexual tissues of prion infected WTD bucks. Future studies will be necessary to determine whether sexual contact and/or artificial inseminations are plausible means of CWD transmission in susceptible animal species. snip... In terms of disease transmission, the presence of prions in semen begs the question on whether sexual contact is plausible route of CWD transmission. A previous report showed that semen collected from rams at pre-clinical and clinical stages of prion disease did not infect scrapie-susceptible mice [34]. Our previous results in Syrian hamsters showed that sexual exposure of naïve females to 263K infected males was ineffective in transmitting disease [35]. Maternal transmission has also be presented as a viable mode of CWD transmission to offspring. Evidence derived from scrapie-infected sheep and experimentally infected muntjac deer provides direct evidence that offspring from infected dams and ewes are at higher risk of developing prion disease [16,36]. Considering the results presented in this article, the risk of CWD transmission via semen cannot be dismiss without further inquiry.
It is important to note that some semen samples tested in the current report showed PrPSc presence after only one PMCA round, suggesting that PrPSc content in semen of some animals may be relatively high. This is particularly relevant considering that tissues from male sexual organs inhibited PMCA performance. It remains unclear if vaginal exposure to CWD prions in semen is an effective route of transmission.
In summary, our results confirm the presence of CWD prions in semen and male sexual tissues in CWD-infected WTD. Future experiments in actual deer will determine whether CWD can be transmitted by breeding practices including sexual contacts or artificial inseminations. Infectivity studies in transgenic mice underway in our laboratory will determine the infectivity titers of some of the samples described in this study.
Friday, February 21, 2025
***> LEGISLATING CWD TSE Prion, Bills to release Genetically Modified Cervid into the wild, what could go wrong?
Friday, February 21, 2025
CWD, BAITING, AND MINERAL LICKS, WHAT IF?
Friday, February 21, 2025
Deer don’t die from CWD, it’s the insurance companies, or it's a Government conspiracy?
Friday, February 21, 2025
Distribution of Chronic Wasting Disease in North America February 2025
THURSDAY, APRIL 10, 2025
***> CWD TSE Prion, Politics, Friendly Fire, Unforeseen Consequences, What If?
Texas S.B. 2843 Directs TPWD to conduct a comprehensive study of current measures to control chronic wasting disease (CWD) in deer
Trying to legislate CWD is what got Texas in this CWD mess to begin with, how did that work out$$$ Legislators and Politicians need to stay away and let TPWD and TAHC et try and contain this mess that Legislators and Politicians got us in, called CWD TSE Prion…terry
Terry S. Singeltary Sr.

posted by Terry S. Singeltary Sr. at 12:47 PM

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