Sunday, January 14, 2018

Michigan’s Chronic Wasting Disease Working Group Recommendations Report to the Natural Resources Commission Prepared December 2017

Michigan’s Chronic Wasting Disease Working Group

Recommendations Report to the
Natural Resources Commission Prepared December 2017

Executive Summary

A Chronic Wasting Disease Working Group was established in response to a charge by Michigan’s Natural Resources Commission (Page 4) to develop recommendations on further steps and actions to substantially mitigate or eliminate CWD in Michigan. The Working Group met on three separate occasions to accomplish this charge and they should be commended for their time and thoughtful contributions to this report.
There are four major areas identified by the Working Group that should be the focus for CWD efforts in Michigan: Communication, Research Consortium, Farmed Cervidae, and Harvest and Removal. From these four thematic areas came five key recommendations, outlined below.

Due to the complexity of CWD, and the importance of communications, the Working Group recommends:

1. The Natural Resources Commission should pursue the help of an outside marketing agency in development of messaging Michigan’s vision for CWD Surveillance and Management.

This messaging should place emphasis on the importance of herd health and Michigan’s commitment to sustainable, healthy deer populations across the state.
The lack of funding is a significant detriment to the fight against CWD, and research is needed to develop new tools and improve epidemiologic understanding of the disease. Therefore,

2. The Natural Resources Commission should take the lead in forming a consortium of states and provinces to seek federal, state and private funding and share research on CWD surveillance methods, diagnostic tools, transmission pathways and management practices.

CWD is a disease that can affect both wild and captive deer in Michigan. Therefore,
3. The Natural Resources Commission should work cooperatively with the Agriculture and Rural Development Commission to assess the effectiveness and direction of the farmed cervidae program, with a focus on biosecurity and CWD risk factors.
Regulations for disease mitigation should proactively address risk with the best available science. Therefore,

4. The Natural Resources Commission should continue to employ science-based strategy for the regulatory framework of CWD management.
Finally, CWD surveillance and management plans should be grounded in science, and consider social, economic, and recreational interests. Therefore,

5. The Natural Resources Commission should develop statewide, science-based management plans based on regional prevalences of CWD.
Hunters and cervid producers should be engaged in the development of the plans.

Snip...see full report;


SUNDAY, JANUARY 14, 2018

Michigan CWD TSE Prion Cases Increase By 6 to 56 Cases To Date



2017

Subject: ***CDC Now Recommends Strongly consider having the deer or elk tested for CWD before you eat the meat

CDC Now Recommends Strongly consider having the deer or elk tested for CWD before you eat the meat 

Chronic Wasting Disease (CWD) 

Prevention 

If CWD could spread to people, it would most likely be through eating of infected deer and elk. In a 2006-2007 CDC survey of U.S. residents, nearly 20 percent of those surveyed said they had hunted deer or elk and more than two-thirds said they had eaten venison or elk meat. However, to date, no CWD infections have been reported in people. 

Hunters must consider many factors when determining whether to eat meat from deer and elk harvested from areas with CWD, including the level of risk they are willing to accept. Hunters harvesting wild deer and elk from areas with reported CWD should check state wildlife and public health guidance to see whether testing of animals is recommended or required in a given state or region. In areas where CWD is known to be present, CDC recommends that hunters strongly consider having those animals tested before eating the meat. 

Tests for CWD are monitoring tools that some state wildlife officials use to look at the rates of CWD in certain animal populations. Testing may not be available in every state, and states may use these tests in different ways. A negative test result does not guarantee that an individual animal is not infected with CWD, but it does make it considerably less likely and may reduce your risk of exposure to CWD. 

To be as safe as possible and decrease their potential risk of exposure to CWD, hunters should take the following steps when hunting in areas with CWD: 

Do not shoot, handle or eat meat from deer and elk that look sick or are acting strangely or are found dead (road-kill). When field-dressing a deer: Wear latex or rubber gloves when dressing the animal or handling the meat. Minimize how much you handle the organs of the animal, particularly the brain or spinal cord tissues. Do not use household knives or other kitchen utensils for field dressing. Check state wildlife and public health guidance to see whether testing of animals is recommended or required. Recommendations vary by state, but information about testing is available from many state wildlife agencies. Strongly consider having the deer or elk tested for CWD before you eat the meat. If you have your deer or elk commercially processed, consider asking that your animal be processed individually to avoid mixing meat from multiple animals. If your animal tests positive for CWD, do not eat meat from that animal. The U.S. Department of Agriculture’s Animal and Plant Health Inspection Service regulates commercially farmed deer and elk. The agency operates a national CWD herd certification program. As part of the voluntary program, states and individual herd owners agree to meet requirements meant to decrease the risk of CWD in their herds. Privately owned herds that do not participate in the herd certification program may be at increased risk for CWD. 

Page last reviewed: August 17, 2017 Page last updated: August 17, 2017 Content source: Centers for Disease Control and Prevention National Center for Emerging and Zoonotic Infectious Diseases (NCEZID) Division of High-Consequence Pathogens and Pathology (DHCPP) 


 > However, to date, no CWD infections have been reported in people. 

key word here is 'reported'. science has shown that CWD in humans will look like sporadic CJD. SO, how can one assume that CWD has not already transmitted to humans? they can't, and it's as simple as that. from all recorded science to date, CWD has already transmitted to humans, and it's being misdiagnosed as sporadic CJD. ...terry 

LOOKING FOR CWD IN HUMANS AS nvCJD or as an ATYPICAL CJD, LOOKING IN ALL THE WRONG PLACES $$$ 

*** These results would seem to suggest that CWD does indeed have zoonotic potential, at least as judged by the compatibility of CWD prions and their human PrPC target. Furthermore, extrapolation from this simple in vitro assay suggests that if zoonotic CWD occurred, it would most likely effect those of the PRNP codon 129-MM genotype and that the PrPres type would be similar to that found in the most common subtype of sCJD (MM1).*** 



Molecular Barriers to Zoonotic Transmission of Prions 

*** chronic wasting disease, there was no absolute barrier to conversion of the human prion protein. 

*** Furthermore, the form of human PrPres produced in this in vitro assay when seeded with CWD, resembles that found in the most common human prion disease, namely sCJD of the MM1 subtype. 


TUESDAY, SEPTEMBER 12, 2017 

CDC Now Recommends Strongly consider having the deer or elk tested for CWD before you eat the meat 


Prion 2017 Conference Abstracts CWD
 2017 PRION CONFERENCE 

First evidence of intracranial and peroral transmission of Chronic Wasting Disease (CWD) into Cynomolgus macaques: a work in progress 

Stefanie Czub1, Walter Schulz-Schaeffer2, Christiane Stahl-Hennig3, Michael Beekes4, Hermann Schaetzl5 and Dirk Motzkus6 1 

University of Calgary Faculty of Veterinary Medicine/Canadian Food Inspection Agency; 2Universitatsklinikum des Saarlandes und Medizinische Fakultat der Universitat des Saarlandes; 3 Deutsches Primaten Zentrum/Goettingen; 4 Robert-Koch-Institut Berlin; 5 University of Calgary Faculty of Veterinary Medicine; 6 presently: Boehringer Ingelheim Veterinary Research Center; previously: Deutsches Primaten Zentrum/Goettingen 

This is a progress report of a project which started in 2009. 21 cynomolgus macaques were challenged with characterized CWD material from white-tailed deer (WTD) or elk by intracerebral (ic), oral, and skin exposure routes. Additional blood transfusion experiments are supposed to assess the CWD contamination risk of human blood product. Challenge materials originated from symptomatic cervids for ic, skin scarification and partially per oral routes (WTD brain). Challenge material for feeding of muscle derived from preclinical WTD and from preclinical macaques for blood transfusion experiments. We have confirmed that the CWD challenge material contained at least two different CWD agents (brain material) as well as CWD prions in muscle-associated nerves. 

Here we present first data on a group of animals either challenged ic with steel wires or per orally and sacrificed with incubation times ranging from 4.5 to 6.9 years at postmortem. Three animals displayed signs of mild clinical disease, including anxiety, apathy, ataxia and/or tremor. In four animals wasting was observed, two of those had confirmed diabetes. All animals have variable signs of prion neuropathology in spinal cords and brains and by supersensitive IHC, reaction was detected in spinal cord segments of all animals. Protein misfolding cyclic amplification (PMCA), real-time quaking-induced conversion (RT-QuiC) and PET-blot assays to further substantiate these findings are on the way, as well as bioassays in bank voles and transgenic mice. 

At present, a total of 10 animals are sacrificed and read-outs are ongoing. Preclinical incubation of the remaining macaques covers a range from 6.4 to 7.10 years. Based on the species barrier and an incubation time of > 5 years for BSE in macaques and about 10 years for scrapie in macaques, we expected an onset of clinical disease beyond 6 years post inoculation. 

PRION 2017 DECIPHERING NEURODEGENERATIVE DISORDERS 

Subject: PRION 2017 CONFERENCE DECIPHERING NEURODEGENERATIVE DISORDERS VIDEO 

PRION 2017 CONFERENCE DECIPHERING NEURODEGENERATIVE DISORDERS 

*** PRION 2017 CONFERENCE VIDEO 



 TUESDAY, JUNE 13, 2017

PRION 2017 CONFERENCE ABSTRACT 

First evidence of intracranial and peroral transmission of Chronic Wasting Disease (CWD) into Cynomolgus macaques: a work in progress


TUESDAY, JULY 04, 2017

*** PRION 2017 CONFERENCE ABSTRACTS ON CHRONIC WASTING DISEASE CWD TSE PRION ***


TUESDAY, JUNE 13, 2017

PRION 2017 CONFERENCE ABSTRACT Chronic Wasting Disease in European moose is associated with PrPSc features different from North American CWD


Wednesday, May 24, 2017 

PRION2017 CONFERENCE VIDEO UPDATE 23 – 26 May 2017 Edinburgh UPDATE 1 


SATURDAY, JULY 29, 2017 

Risk Advisory Opinion: Potential Human Health Risks from Chronic Wasting Disease CFIA, PHAC, HC (HPFB and FNIHB), INAC, Parks Canada, ECCC and AAFC 



***Moreover, sporadic disease has never been observed in breeding colonies or primate research laboratories, most notably among hundreds of animals over several decades of study at the National Institutes of Health25, and in nearly twenty older animals continuously housed in our own facility.*** 


 
*** Infectious agent of sheep scrapie may persist in the environment for at least 16 years *** 

Gudmundur Georgsson1, Sigurdur Sigurdarson2 and Paul Brown3 



 
Using in vitro prion replication for high sensitive detection of prions and prionlike proteins and for understanding mechanisms of transmission.

 
Claudio Soto

 
Mitchell Center for Alzheimer's diseases and related Brain disorders, Department of Neurology, University of Texas Medical School at Houston.

 
Prion and prion-like proteins are misfolded protein aggregates with the ability to selfpropagate to spread disease between cells, organs and in some cases across individuals. I n T r a n s m i s s i b l e s p o n g i f o r m encephalopathies (TSEs), prions are mostly composed by a misfolded form of the prion protein (PrPSc), which propagates by transmitting its misfolding to the normal prion protein (PrPC). The availability of a procedure to replicate prions in the laboratory may be important to study the mechanism of prion and prion-like spreading and to develop high sensitive detection of small quantities of misfolded proteins in biological fluids, tissues and environmental samples. Protein Misfolding Cyclic Amplification (PMCA) is a simple, fast and efficient methodology to mimic prion replication in the test tube. PMCA is a platform technology that may enable amplification of any prion-like misfolded protein aggregating through a seeding/nucleation process. In TSEs, PMCA is able to detect the equivalent of one single molecule of infectious PrPSc and propagate prions that maintain high infectivity, strain properties and species specificity. Using PMCA we have been able to detect PrPSc in blood and urine of experimentally infected animals and humans affected by vCJD with high sensitivity and specificity. Recently, we have expanded the principles of PMCA to amplify amyloid-beta (Aβ) and alphasynuclein (α-syn) aggregates implicated in Alzheimer's and Parkinson's diseases, respectively. Experiments are ongoing to study the utility of this technology to detect Aβ and α-syn aggregates in samples of CSF and blood from patients affected by these diseases.

=========================

***Recently, we have been using PMCA to study the role of environmental prion contamination on the horizontal spreading of TSEs. These experiments have focused on the study of the interaction of prions with plants and environmentally relevant surfaces. Our results show that plants (both leaves and roots) bind tightly to prions present in brain extracts and excreta (urine and feces) and retain even small quantities of PrPSc for long periods of time. Strikingly, ingestion of prioncontaminated leaves and roots produced disease with a 100% attack rate and an incubation period not substantially longer than feeding animals directly with scrapie brain homogenate. Furthermore, plants can uptake prions from contaminated soil and transport them to different parts of the plant tissue (stem and leaves). Similarly, prions bind tightly to a variety of environmentally relevant surfaces, including stones, wood, metals, plastic, glass, cement, etc. Prion contaminated surfaces efficiently transmit prion disease when these materials were directly injected into the brain of animals and strikingly when the contaminated surfaces were just placed in the animal cage. These findings demonstrate that environmental materials can efficiently bind infectious prions and act as carriers of infectivity, suggesting that they may play an important role in the horizontal transmission of the disease.

========================


Since its invention 13 years ago, PMCA has helped to answer fundamental questions of prion propagation and has broad applications in research areas including the food industry, blood bank safety and human and veterinary disease diagnosis. 



Friday, December 14, 2012

DEFRA U.K. What is the risk of Chronic Wasting Disease CWD being introduced into Great Britain? A Qualitative Risk Assessment October 2012

snip...

In the USA, under the Food and Drug Administration's BSE Feed Regulation (21 CFR 589.2000) most material (exceptions include milk, tallow, and gelatin) from deer and elk is prohibited for use in feed for ruminant animals. With regards to feed for non-ruminant animals, under FDA law, CWD positive deer may not be used for any animal feed or feed ingredients. For elk and deer considered at high risk for CWD, the FDA recommends that these animals do not enter the animal feed system. However, this recommendation is guidance and not a requirement by law.

Animals considered at high risk for CWD include:

1) animals from areas declared to be endemic for CWD and/or to be CWD eradication zones and

2) deer and elk that at some time during the 60-month period prior to slaughter were in a captive herd that contained a CWD-positive animal.

Therefore, in the USA, materials from cervids other than CWD positive animals may be used in animal feed and feed ingredients for non-ruminants.

The amount of animal PAP that is of deer and/or elk origin imported from the USA to GB can not be determined, however, as it is not specified in TRACES. It may constitute a small percentage of the 8412 kilos of non-fish origin processed animal proteins that were imported from US into GB in 2011.

Overall, therefore, it is considered there is a __greater than negligible risk___ that (nonruminant) animal feed and pet food containing deer and/or elk protein is imported into GB.

There is uncertainty associated with this estimate given the lack of data on the amount of deer and/or elk protein possibly being imported in these products.

snip...

36% in 2007 (Almberg et al., 2011). In such areas, population declines of deer of up to 30 to 50% have been observed (Almberg et al., 2011). In areas of Colorado, the prevalence can be as high as 30% (EFSA, 2011).

The clinical signs of CWD in affected adults are weight loss and behavioural changes that can span weeks or months (Williams, 2005). In addition, signs might include excessive salivation, behavioural alterations including a fixed stare and changes in interaction with other animals in the herd, and an altered stance (Williams, 2005). These signs are indistinguishable from cervids experimentally infected with bovine spongiform encephalopathy (BSE).

Given this, if CWD was to be introduced into countries with BSE such as GB, for example, infected deer populations would need to be tested to differentiate if they were infected with CWD or BSE to minimise the risk of BSE entering the human food-chain via affected venison.

snip...

The rate of transmission of CWD has been reported to be as high as 30% and can approach 100% among captive animals in endemic areas (Safar et al., 2008).

snip...

In summary, in endemic areas, there is a medium probability that the soil and surrounding environment is contaminated with CWD prions and in a bioavailable form. In rural areas where CWD has not been reported and deer are present, there is a greater than negligible risk the soil is contaminated with CWD prion.

snip...

In summary, given the volume of tourists, hunters and servicemen moving between GB and North America, the probability of at least one person travelling to/from a CWD affected area and, in doing so, contaminating their clothing, footwear and/or equipment prior to arriving in GB is greater than negligible. For deer hunters, specifically, the risk is likely to be greater given the increased contact with deer and their environment. However, there is significant uncertainty associated with these estimates.

snip...

Therefore, it is considered that farmed and park deer may have a higher probability of exposure to CWD transferred to the environment than wild deer given the restricted habitat range and higher frequency of contact with tourists and returning GB residents.

snip...

http://www.defra.gov.uk/animal-diseases/files/qra_chronic-wasting-disease-121029.pdf

TSS

Singeltary submission ;

Program Standards: Chronic Wasting Disease Herd Certification Program and Interstate Movement of Farmed or Captive Deer, Elk, and Moose

*** DOCUMENT ID: APHIS-2006-0118-0411

http://www.regulations.gov/#!documentDetail;D=APHIS-2006-0118-0411


http://chronic-wasting-disease.blogspot.com/2014/03/docket-no-00-108-10-chronic-wasting.html

Back around 2000, 2001, or so, I was corresponding with officials abroad during the bse inquiry, passing info back and forth, and some officials from here inside USDA aphis FSIS et al. In fact helped me get into the USA 50 state emergency BSE conference call way back. That one was a doozy. But I always remember what “deep throat” I never knew who they were, but I never forgot;

Some unofficial information from a source on the inside looking out -

Confidential!!!!

As early as 1992-3 there had been long studies conducted on small pastures containing scrapie infected sheep at the sheep research station associated with the Neuropathogenesis Unit in Edinburgh, Scotland. Whether these are documented...I don't know. But personal recounts both heard and recorded in a daily journal indicate that leaving the pastures free and replacing the topsoil completely at least 2 feet of thickness each year for SEVEN years....and then when very clean (proven scrapie free) sheep were placed on these small pastures.... the new sheep also broke out with scrapie and passed it to offspring. I am not sure that TSE contaminated ground could ever be free of the agent!! A very frightening revelation!!!

---end personal email---end...tss

Terry S. Singeltary Sr.

Michigan CWD TSE Prion Cases Increase By 6 to 56 Cases To Date


CWD Testing Status Update - As of January 10, 2017

Deer Tested for Chronic Wasting Disease Since Detection of First Positive Free-ranging Deer (May 2015)
 
 Targeted DeerRoadkill Deer
or Found Dead
Disease Control and
Crop Damage Permits
Culled By
Wildlife Services
Hunter
Harvested Deer
TotalSuspect/CWD-Positive Deer
DMU 333 (20-Township)812594133214137237126579
DMU 419 (5-County)843382107371943580
Kent County151119050955410
Mecosta County14142494121200027711
Montcalm County 2810434903236371936
Western UP (4-County)72151017026014990
Remainder of State40112525181254333990
Totals: 630352936721622195042895756

Counties and Townships with CWD Suspect or Positive Results

Clinton County
  • Dewitt Township
  • Eagle Township
  • Watertown Township
Ingham County
  • Meridian Township
Kent County
  • Oakfield Township
  • Spencer Township
Mecosta
  • Aetna Township
Montcalm County
  • Cato Township
  • Douglas Township
  • Fairplain Township
  • Ferris Township
  • Maple Valley Township
  • Montcalm Township
  • Pine Township
  • Reynolds Township
  • Sidney Township
  • Winfield Township 



THURSDAY, DECEMBER 28, 2017

MICHIGAN CWD TSE PRION AKA MAD DEER DISEASE CASES CONTINUE TO MOUNT INCREASE TO 50 CASES TO DATE


FRIDAY, DECEMBER 22, 2017 

Michigan Chronic Wasting Disease CWD TSE Prion now at 48 cases As of December 20, 2017


WEDNESDAY, DECEMBER 06, 2017 

Michigan DNR CWD INCREASES TO TOTAL 30 CASES TO DATE


FRIDAY, NOVEMBER 17, 2017 

Michigan 11 case CWD confirmed, three additional suspect positives awaiting confirmation


TUESDAY, DECEMBER 12, 2017 

Chronic Wasting Disease CWD TSE Prion (aka mad deer disease) Update USA December 14, 2017 


WEDNESDAY, DECEMBER 13, 2017 

Michigan Chronic Wasting Disease Identified in a Mecosta County Farmed Deer


FRIDAY, DECEMBER 15, 2017

Canada CFIA updating its national CWD TSE PRION efforts to eradicate disease farmed cervid NOT successful December 14, 2017


CDC STRONGLY SUGGEST IN 2017;

Do not shoot, handle or eat meat from deer and elk that look sick or are acting strangely or are found dead (road-kill). When field-dressing a deer: Wear latex or rubber gloves when dressing the animal or handling the meat. Minimize how much you handle the organs of the animal, particularly the brain or spinal cord tissues. Do not use household knives or other kitchen utensils for field dressing. Check state wildlife and public health guidance to see whether testing of animals is recommended or required. Recommendations vary by state, but information about testing is available from many state wildlife agencies. Strongly consider having the deer or elk tested for CWD before you eat the meat. If you have your deer or elk commercially processed, consider asking that your animal be processed individually to avoid mixing meat from multiple animals. If your animal tests positive for CWD, do not eat meat from that animal. 

SEE FULL REPORT BELOW!

SUNDAY, DECEMBER 10, 2017 

Detection of Prions in Blood of Cervids at the Asymptomatic Stage of Chronic Wasting Disease

The results showed a sensitivity of 100% in animals with very poor body condition that were IHC-positive in both brain and lymph nodes, 96% in asymptomatic deer IHC-positive in brain and lymph nodes and 53% in animals at early stages of infection that were IHC-positive only in lymph nodes. The overall mean diagnostic sensitivity was 79.3% with 100% specificity. These findings show that PMCA might be useful as a blood test for routine, live animal diagnosis of CWD.



CHRONIC WASTING DISEASE CWD TSE PRION ZOONOTIC ZOONOSIS

PRICE OF TSE PRION POKER GOES UP!

2017

Subject: ***CDC Now Recommends Strongly consider having the deer or elk tested for CWD before you eat the meat

CDC Now Recommends Strongly consider having the deer or elk tested for CWD before you eat the meat 

Chronic Wasting Disease (CWD) 

Prevention 

If CWD could spread to people, it would most likely be through eating of infected deer and elk. In a 2006-2007 CDC survey of U.S. residents, nearly 20 percent of those surveyed said they had hunted deer or elk and more than two-thirds said they had eaten venison or elk meat. However, to date, no CWD infections have been reported in people. 

Hunters must consider many factors when determining whether to eat meat from deer and elk harvested from areas with CWD, including the level of risk they are willing to accept. Hunters harvesting wild deer and elk from areas with reported CWD should check state wildlife and public health guidance to see whether testing of animals is recommended or required in a given state or region. In areas where CWD is known to be present, CDC recommends that hunters strongly consider having those animals tested before eating the meat. 

Tests for CWD are monitoring tools that some state wildlife officials use to look at the rates of CWD in certain animal populations. Testing may not be available in every state, and states may use these tests in different ways. A negative test result does not guarantee that an individual animal is not infected with CWD, but it does make it considerably less likely and may reduce your risk of exposure to CWD. 

To be as safe as possible and decrease their potential risk of exposure to CWD, hunters should take the following steps when hunting in areas with CWD: 

Do not shoot, handle or eat meat from deer and elk that look sick or are acting strangely or are found dead (road-kill). When field-dressing a deer: Wear latex or rubber gloves when dressing the animal or handling the meat. Minimize how much you handle the organs of the animal, particularly the brain or spinal cord tissues. Do not use household knives or other kitchen utensils for field dressing. Check state wildlife and public health guidance to see whether testing of animals is recommended or required. Recommendations vary by state, but information about testing is available from many state wildlife agencies. Strongly consider having the deer or elk tested for CWD before you eat the meat. If you have your deer or elk commercially processed, consider asking that your animal be processed individually to avoid mixing meat from multiple animals. If your animal tests positive for CWD, do not eat meat from that animal. The U.S. Department of Agriculture’s Animal and Plant Health Inspection Service regulates commercially farmed deer and elk. The agency operates a national CWD herd certification program. As part of the voluntary program, states and individual herd owners agree to meet requirements meant to decrease the risk of CWD in their herds. Privately owned herds that do not participate in the herd certification program may be at increased risk for CWD. 

Page last reviewed: August 17, 2017 Page last updated: August 17, 2017 Content source: Centers for Disease Control and Prevention National Center for Emerging and Zoonotic Infectious Diseases (NCEZID) Division of High-Consequence Pathogens and Pathology (DHCPP) 


 > However, to date, no CWD infections have been reported in people. 

key word here is 'reported'. science has shown that CWD in humans will look like sporadic CJD. SO, how can one assume that CWD has not already transmitted to humans? they can't, and it's as simple as that. from all recorded science to date, CWD has already transmitted to humans, and it's being misdiagnosed as sporadic CJD. ...terry 

LOOKING FOR CWD IN HUMANS AS nvCJD or as an ATYPICAL CJD, LOOKING IN ALL THE WRONG PLACES $$$ 

*** These results would seem to suggest that CWD does indeed have zoonotic potential, at least as judged by the compatibility of CWD prions and their human PrPC target. Furthermore, extrapolation from this simple in vitro assay suggests that if zoonotic CWD occurred, it would most likely effect those of the PRNP codon 129-MM genotype and that the PrPres type would be similar to that found in the most common subtype of sCJD (MM1).*** 




TUESDAY, SEPTEMBER 12, 2017 

CDC Now Recommends Strongly consider having the deer or elk tested for CWD before you eat the meat 


Prion 2017 Conference Abstracts CWD
 2017 PRION CONFERENCE 

First evidence of intracranial and peroral transmission of Chronic Wasting Disease (CWD) into Cynomolgus macaques: a work in progress 

Stefanie Czub1, Walter Schulz-Schaeffer2, Christiane Stahl-Hennig3, Michael Beekes4, Hermann Schaetzl5 and Dirk Motzkus6 1 

University of Calgary Faculty of Veterinary Medicine/Canadian Food Inspection Agency; 2Universitatsklinikum des Saarlandes und Medizinische Fakultat der Universitat des Saarlandes; 3 Deutsches Primaten Zentrum/Goettingen; 4 Robert-Koch-Institut Berlin; 5 University of Calgary Faculty of Veterinary Medicine; 6 presently: Boehringer Ingelheim Veterinary Research Center; previously: Deutsches Primaten Zentrum/Goettingen 

This is a progress report of a project which started in 2009. 21 cynomolgus macaques were challenged with characterized CWD material from white-tailed deer (WTD) or elk by intracerebral (ic), oral, and skin exposure routes. Additional blood transfusion experiments are supposed to assess the CWD contamination risk of human blood product. Challenge materials originated from symptomatic cervids for ic, skin scarification and partially per oral routes (WTD brain). Challenge material for feeding of muscle derived from preclinical WTD and from preclinical macaques for blood transfusion experiments. We have confirmed that the CWD challenge material contained at least two different CWD agents (brain material) as well as CWD prions in muscle-associated nerves. 

Here we present first data on a group of animals either challenged ic with steel wires or per orally and sacrificed with incubation times ranging from 4.5 to 6.9 years at postmortem. Three animals displayed signs of mild clinical disease, including anxiety, apathy, ataxia and/or tremor. In four animals wasting was observed, two of those had confirmed diabetes. All animals have variable signs of prion neuropathology in spinal cords and brains and by supersensitive IHC, reaction was detected in spinal cord segments of all animals. Protein misfolding cyclic amplification (PMCA), real-time quaking-induced conversion (RT-QuiC) and PET-blot assays to further substantiate these findings are on the way, as well as bioassays in bank voles and transgenic mice. 

At present, a total of 10 animals are sacrificed and read-outs are ongoing. Preclinical incubation of the remaining macaques covers a range from 6.4 to 7.10 years. Based on the species barrier and an incubation time of > 5 years for BSE in macaques and about 10 years for scrapie in macaques, we expected an onset of clinical disease beyond 6 years post inoculation. 

PRION 2017 DECIPHERING NEURODEGENERATIVE DISORDERS 

Subject: PRION 2017 CONFERENCE DECIPHERING NEURODEGENERATIVE DISORDERS VIDEO 

PRION 2017 CONFERENCE DECIPHERING NEURODEGENERATIVE DISORDERS 

*** PRION 2017 CONFERENCE VIDEO 



TUESDAY, JULY 04, 2017

*** PRION 2017 CONFERENCE ABSTRACTS ON CHRONIC WASTING DISEASE CWD TSE PRION ***


 the tse prion aka mad cow type disease is not your normal pathogen. 
The TSE prion disease survives ashing to 600 degrees celsius, that’s around 1112 degrees farenheit. 

you cannot cook the TSE prion disease out of meat. 

you can take the ash and mix it with saline and inject that ash into a mouse, and the mouse will go down with TSE. 

Prion Infected Meat-and-Bone Meal Is Still Infectious after Biodiesel Production as well. 

the TSE prion agent also survives Simulated Wastewater Treatment Processes. 

IN fact, you should also know that the TSE Prion agent will survive in the environment for years, if not decades. 

you can bury it and it will not go away. 

The TSE agent is capable of infected your water table i.e. Detection of protease-resistant cervid prion protein in water from a CWD-endemic area. 

it’s not your ordinary pathogen you can just cook it out and be done with. 

that’s what’s so worrisome about Iatrogenic mode of transmission, a simple autoclave will not kill this TSE prion agent.

1: J Neurol Neurosurg Psychiatry 1994 Jun;57(6):757-8 

Transmission of Creutzfeldt-Jakob disease to a chimpanzee by electrodes contaminated during neurosurgery. 

Gibbs CJ Jr, Asher DM, Kobrine A, Amyx HL, Sulima MP, Gajdusek DC. 

Laboratory of Central Nervous System Studies, National Institute of 

Neurological Disorders and Stroke, National Institutes of Health, 

Bethesda, MD 20892. 

Stereotactic multicontact electrodes used to probe the cerebral cortex of a middle aged woman with progressive dementia were previously implicated in the accidental transmission of Creutzfeldt-Jakob disease (CJD) to two younger patients. The diagnoses of CJD have been confirmed for all three cases. More than two years after their last use in humans, after three cleanings and repeated sterilisation in ethanol and formaldehyde vapour, the electrodes were implanted in the cortex of a chimpanzee. Eighteen months later the animal became ill with CJD. This finding serves to re-emphasise the potential danger posed by reuse of instruments contaminated with the agents of spongiform encephalopathies, even after scrupulous attempts to clean them. 

PMID: 8006664 [PubMed - indexed for MEDLINE] 


New studies on the heat resistance of hamster-adapted scrapie agent: Threshold survival after ashing at 600°C suggests an inorganic template of replication 


Prion Infected Meat-and-Bone Meal Is Still Infectious after Biodiesel Production 


Detection of protease-resistant cervid prion protein in water from a CWD-endemic area 


A Quantitative Assessment of the Amount of Prion Diverted to Category 1 Materials and Wastewater During Processing 


Rapid assessment of bovine spongiform encephalopathy prion inactivation by heat treatment in yellow grease produced in the industrial manufacturing process of meat and bone meals 


PPo4-4: 

Survival and Limited Spread of TSE Infectivity after Burial 



URINE

SUNDAY, JULY 16, 2017

*** Temporal patterns of chronic wasting disease prion excretion in three cervid species ***


TITLE: PATHOLOGICAL FEATURES OF CHRONIC WASTING DISEASE IN REINDEER AND DEMONSTRATION OF HORIZONTAL TRANSMISSION 

 
*** DECEMBER 2016 CDC EMERGING INFECTIOUS DISEASE JOURNAL CWD HORIZONTAL TRANSMISSION 


Using in vitro prion replication for high sensitive detection of prions and prionlike proteins and for understanding mechanisms of transmission. 
 
Claudio Soto Mitchell Center for Alzheimer's diseases and related Brain disorders, Department of Neurology, University of Texas Medical School at Houston. 
 
***Recently, we have been using PMCA to study the role of environmental prion contamination on the horizontal spreading of TSEs. These experiments have focused on the study of the interaction of prions with plants and environmentally relevant surfaces. Our results show that plants (both leaves and roots) bind tightly to prions present in brain extracts and excreta (urine and feces) and retain even small quantities of PrPSc for long periods of time. Strikingly, ingestion of prioncontaminated leaves and roots produced disease with a 100% attack rate and an incubation period not substantially longer than feeding animals directly with scrapie brain homogenate. Furthermore, plants can uptake prions from contaminated soil and transport them to different parts of the plant tissue (stem and leaves). Similarly, prions bind tightly to a variety of environmentally relevant surfaces, including stones, wood, metals, plastic, glass, cement, etc. Prion contaminated surfaces efficiently transmit prion disease when these materials were directly injected into the brain of animals and strikingly when the contaminated surfaces were just placed in the animal cage. These findings demonstrate that environmental materials can efficiently bind infectious prions and act as carriers of infectivity, suggesting that they may play an important role in the horizontal transmission of the disease. 
 
======================== 
 
Since its invention 13 years ago, PMCA has helped to answer fundamental questions of prion propagation and has broad applications in research areas including the food industry, blood bank safety and human and veterinary disease diagnosis. 
 
 
 
In conclusion, the results in the current study indicate that removal of furniture that had been in contact with scrapie-infected animals should be recommended, particularly since cleaning and decontamination may not effectively remove scrapie infectivity (31), even though infectivity declines considerably if the pasture and the field furniture have not been in contact with scrapie-infected sheep for several months. As sPMCA failed to detect PrPSc in furniture that was subjected to weathering, even though exposure led to infection in sheep, this method may not always be reliable in predicting the risk of scrapie infection through environmental contamination. These results suggest that the VRQ/VRQ sheep model may be more sensitive than sPMCA for the detection of environmentally associated scrapie, and suggest that extremely low levels of scrapie contamination are able to cause infection in susceptible sheep genotypes. 
 
Keywords: classical scrapie, prion, transmissible spongiform encephalopathy, sheep, field furniture, reservoir, serial protein misfolding cyclic amplification 
 
 
Wednesday, December 16, 2015 
 
*** Objects in contact with classical scrapie sheep act as a reservoir for scrapie transmission *** 
 
 
*** Infectious agent of sheep scrapie may persist in the environment for at least 16 years *** 
 
Gudmundur Georgsson1, Sigurdur Sigurdarson2 and Paul Brown3 
 
Subject:

 CWD MICHIGAN UPDATE September 5, 2008

From:

 TERRY SINGELTARY

Reply-To:

 Bovine Spongiform Encephalopathy

Date:

 Tue, 9 Sep 2008 14:36:36 -0500

-------------------- BSE-L@LISTS.AEGEE.ORG -------------------- 

CWD Update Chronic Wasting Disease Eradication Program Provided by the Animal Industry Division Michigan Department of Agriculture September 5, 2008

Background: The Michigan departments of Agriculture (MDA) and Natural Resources (DNR) confirmed the state's first case of Chronic Wasting Disease (CWD) in a three-year old white-tailed deer from a privately owned cervid (POC) facility in Kent County on Monday, August 25, 2008. The state quarantined all POC facilities, prohibiting the movement of all - dead or alive - privately-owned deer, elk, or moose. Officials do not yet know how the deer may have contracted the disease. To date, there is no evidence that CWD presents a risk to humans or to animals other than cervids. MDA Actions: The state-wide quarantine on all privately owned cervid facilities is still in place. Facilities may continue to hold shooting events, but all carcasses* must be held until testing clears the animal/or the quarantine is released. A clarification to the quarantines was published and distributed to law enforcement officials, stakeholders and other interested parties. A questions and answers sheet is available under the livestock link on the www.michigan.gov/chronicwastingdisease website.

The test results from the Kent County cervid breeding facility, where the index case was confirmed, found no additional diseased deer. Epidemiologists are reviewing taxidermy records on a facility related to the index case. Taxidermy operations must be licensed and operators must follow Michigan requirements when conducting business with hunters who have harvested animals from other states. Current Michigan law prohibits the import of free-ranging deer or elk carcasses from states or provinces with CWD. Only de-boned meat, antlers, antlers attached to a scull cap cleaned of all brain and muscle tissue, hides and upper canine teeth may be brought into Michigan. A person that is notified by mail or other means, that a carcass imported into Michigan tested positive for CWD must notify the Michigan DNR.

The first tier of traces from the index facility led to five facilities: three in Kent County, one in Montcalm County, and one in Osceola County. These facilities were quarantined by MDA. Records of sales and purchases have been reviewed and have revealed that two facilities received deer from the index case. Four deer from these two facilities were 2 euthanized, samples were tested at MSU's DCPAH and were found to be negative on Thursday, September 04, 2008. One of the five facilities in tier one, also a Kent County facility conducts a taxidermy operation on the premises. Taxidermy is of great concern because infectious prions in the bones and spinal tissue of the carcass from CWD positive states can infect deer on the facility. MDA, DNR and USDA staff are investigating the records of the taxidermy operation. The second tier investigation to this point, has quarantined four facilities in Bay, Kent, Mecosta, and Saginaw counties. These facilities only sold to the tier one facilities and did not receive anything. They are quarantined as terminal operations and any deer that die, are culled, or shot for sport must be submitted for CWD testing. POC Facilities Quarantined: All POC facilities, except those that only have reindeer, are under quarantine in Michigan until the disease investigation is complete. Epidemiologists are developing a policy for records review and release of quarantines based on management practices and risk.

Disease Surveillance Table: Index facility Depopulated Tier 1 Tier 2 1 Entire index herd tested negative 5 herds 2 trace outs (four test-negative animals) 3 trace ins 4 none of these 4 facilities trace directly to the index facility DNR Actions: The ban on all baiting and feeding of deer and elk in the Lower Peninsula is in effect. MSU's Product Center for Agriculture Development is taking calls from bait growers/sellers. The Center is using Michigan Market Maker, an interactive mapping system that connects Agriculture processors and businesses with Michigan growers and marketers. http://mi.marketmaker.uiuc.edu/ Information on the baiting and feeding ban is available on the CWD page of the Emerging Diseases website. A mandatory deer check for hunters who take a deer within the Kent County townships of Tyrone, Solon, Nelson, Sparta, Algoma, Courtland, Alpine, Plainfield, and Cannon, is in effect for the 2008 hunting season. The deer heads will be collected and tested for CWD. All transport of live wild deer, elk, and moose is prohibited statewide, including transport for rehabilitation purposes. Education and Outreach: A town hall meeting is scheduled to take place in Kent County on September 9, 2008 at 6:30 p.m. near Grand Rapids. Representatives from MDA, USDA, DNR and MSU will be there to answer questions about CWD, quarantines and the baiting ban. An update on the disease investigation will be presented to the House of Representatives Committee on Outdoor Recreation and Natural Resources on September 9, 2008 and the Senate Natural Resources, Agriculture, and Hunting and Fishing committees on September 10, 2008. 3 Questions and Answers regarding POC facility quarantines were sent via email to legislative offices, POC facility executive directors, and DNR law enforcement. They are also posted to the MDA, and Emerging Diseases websites. A coordinated communications action plan is in place. MSU Extension, Michigan Deer and Elk Breeders, MUCC, and many other special interest groups have volunteered to assist with information distribution. Information on CWD may be found on the Michigan Emerging Diseases website at www.michigan.gov/emergingdiseases. Corrected on September 8, 2008 - changed from "meat" to "carcasses".



Summary of Michigan Wildlife Chronic Wasting Disease Surveillance Updated May 1, 2008 by Michigan Department of Natural Resources, Wildlife Disease Laboratory



snip... 

Monday, August 25, 2008

CWD FIRST DOCUMENTED IN MICHIGAN


2017

In addition, two white-tailed does were submitted for monitoring by a Privately-Owned Cervid (POC) facility in Mecosta County and were positively confirmed with CWD on January 17, 2017. As of April 17, 2017, no additional CWD positive animals have been found in the POC facility or within free-ranging deer in the surrounding areas. 



Back around 2000, 2001, or so, I was corresponding with officials abroad during the bse inquiry, passing info back and forth, and some officials from here inside USDA aphis FSIS et al. In fact helped me get into the USA 50 state emergency BSE conference call way back. That one was a doozy. But I always remember what “deep throat” I never knew who they were, but I never forgot;

Some unofficial information from a source on the inside looking out -

Confidential!!!!

As early as 1992-3 there had been long studies conducted on small pastures containing scrapie infected sheep at the sheep research station associated with the Neuropathogenesis Unit in Edinburgh, Scotland. Whether these are documented...I don't know. But personal recounts both heard and recorded in a daily journal indicate that leaving the pastures free and replacing the topsoil completely at least 2 feet of thickness each year for SEVEN years....and then when very clean (proven scrapie free) sheep were placed on these small pastures.... the new sheep also broke out with scrapie and passed it to offspring. I am not sure that TSE contaminated ground could ever be free of the agent!! A very frightening revelation!!!

---end personal email---end...tss


DEEP THROAT TO TSS 2000-2001

(take these old snips of emails with how ever
many grains of salt you wish. ...tss)

The most frightening thing I have read all day is the
report of Gambetti's finding of a new strain of
sporadic cjd in young people...Dear God, what in
the name of all that is holy is that!!!
If the US has different strains of
scrapie.....why????than the UK...then would the same
mechanisms that make different strains of scrapie here
make different strains of BSE...if the patterns are
different in sheep and mice for scrapie.....could not
the BSE be different in the cattle, in the mink, in
the humans.......I really think the slides or tissues
and everything from these young people with the new
strain of sporadic cjd should be put up to be analyzed
by many, many experts in cjd........bse.....scrapie
Scrape the damn slide and put it into
mice.....wait.....chop up the mouse brain and and
spinal cord........put into some more mice.....dammit
amplify the thing and start the damned
research.....This is NOT rocket science...we need to
use what we know and get off our butts and move....the
whining about how long everything takes.....well it
takes a whole lot longer if you whine for a year and
then start the research!!!

Not sure where I read this but it was a recent press
release or something like that:

I thought I would fall out of my chair when I read
about how there was no worry about infectivity from a
histopath slide or tissues because they are preserved
in formic acid, or formalin or formaldehyde.....for
God's sake........ Ask any pathologist in the UK what
the brain tissues in the formalin looks like after a
year.......it is a big fat sponge...the agent
continues to eat the brain ......you can't make slides
anymore because the agent has never stopped........and
the old slides that are stained with Hemolysin and
Eosin......they get holier and holier and degenerate
and continue...what you looked at 6 months ago is not
there........Gambetti better be photographing every
damned thing he is looking at.....

Okay, you need to know. You don't need to pass it on
as nothing will come of it and there is not a damned
thing anyone can do about it. Don't even hint at it
as it will be denied and laughed at..........
USDA is gonna do as little as possible until there is
actually a human case in the USA of the
nvcjd........if you want to move this thing along and
shake the earth....then we gotta get the victims
families to make sure whoever is doing the autopsy is
credible, trustworthy, and a saint with the courage of
Joan of Arc........I am not kidding!!!!

so, unless we get a human death from EXACTLY the same
form with EXACTLY the same histopath lesions as seen
in the UK nvcjd........forget any action........it is
ALL gonna be sporadic!!!

And, if there is a case.......there is gonna be every
effort to link it to international travel,
international food, etc. etc. etc. etc. etc. They
will go so far as to find out if a sex partner had
ever traveled to the UK/europe, etc. etc. ....
It is gonna be a long, lonely, dangerous twisted
journey to the truth. They have all the cards, all
the money, and are willing to threaten and carry out
those threats....and this may be their biggest
downfall...

Thanks as always for your help.

(Recently had a very startling revelation from a rather senior person in
government here..........knocked me out of my chair........you must keep
pushing. If I was a power person....I would be demanding that there be a
least a million bovine tested as soon as possible and agressively
seeking this disease. The big players are coming out of the woodwork as
there is money to be made!!!

In short: "FIRE AT WILL"!!! for the very dumb....who's "will"! "Will
be the burden to bare if there is any coverup!"

again it was said years ago and it should
be taken seriously....BSE will NEVER be found in the
US!

As for the BSE conference call...I think you did a
great service to freedom of information and making
some people feign integrity...I find it scary to see
that most of the "experts" are employed by the federal
government or are supported on the "teat" of federal
funds. A scary picture!

I hope there is a confidential panel organized by the
new government to really investigate this thing.

You need to watch your back........but keep picking at
them.......like a buzzard to the bone...you just may
get to the truth!!! (You probably have more support than
you know. Too many people are afraid to show you or let
anyone else know. I have heard a few things myself...
you ask the questions that everyone else is too afraid to ask.)

End...tss

http://tseac.blogspot.com/2011/02/usa-50-state-bse-mad-cow-conference.html



kind regards, terry