Transmission of TSEs through ectoparasites has been postulated by Lupi5. Post et al6 fed larvae of meat eating and myiasis causing flies with brain material from scrapie infected hamsters. Two days after eating infected material, the larvae showed high amounts of PrPSc by Western blot. In further studies, the inner organs of larvae, which had been fed with scrapie brain, were extracted and fed to hamsters. Six out of eight hamsters developed scrapie. Two out of four hamsters fed on scrapie infected pupae subsequently developed scrapie.
SEAC 97/2 Annex 3
Published research and Previous SEAC and European Commission DG
Health and Consumer Protection Scientific Steering Committee (SSC)
advice
Sewage sludge
In a June 1996 statement1 , along with recommendations for handling waste material from cattle, SEAC considered the practice of spreading of sewage sludge on land in the context of the risk of Specified Risk Material (SRM) particles from abattoir waste potentially entering the sewerage system. Provided the particulate matter was retained and disposed of as SRM, the committee was content for abattoirs to discharge their liquid waste to sewers and for sewage sludge to be disposed of by spreading on land. Any small particulate matter passing through the trap would be diluted to such an extent as to pose negligible risk.
Gale and Stanfield2 estimated the risks from sewage sludge based on the assumption that 1% of brain and spinal cord is lost to the sewer from abattoirs. The model predicts a risk of BSE transmission of 71 x 10-5 cow-1 year–1 for cattle grazing on land to which sewage sludge has been applied. The authors conclude that the dose consumed by grazing cattle is insufficient to sustain the BSE epidemic in the UK cattle herd. The risk from sewage sludge derived from human, cattle and other species remains theoretical.
Birds
The possibility that birds may act as possible transmitters of BSE was considered by the SSC opinion 7-8 November 20023 (provided) “Necrophagous birds as possible transmitters of TSE/BSE”. The SSC concluded that birds could have theoretically ingested infectious material through fallen stock. It had been proposed that the spread of the ingested infectious material could occur through faecal contamination, as it is unlikely the pathological prion protein would be destroyed in the digestive tract. The SSC concluded that the possibility of active replication of PrPSc in birds is remote but agreed that such pathways of transmission cannot be excluded given these birds cover great distances during migration.
Rodents
Concepcion and Padlan4 have postulated that inadvertent ingestion of infected rodent parts, possibly droppings, may be a potential mode of transmission of TSEs. This postulate is based on sequence homology comparisons, which showed a close similarity between sequences of human and rodent prion proteins in a peptic fragment
1 http://www.seac.gov.uk/statements/state07jun96.htm
2 Gale P. and Stanfield G. (2001) Towards a quantitative risk assessment for BSE in sewage sludge J. Appl. Microbiol. 91, 563-569
3 http://ec.europa.eu/food/fs/sc/ssc/out295_en.pdf
4 Concepcion G.P. and Padlan E.A. (2003) Are humans getting ‘mad cow disease’ from eating beef or something else? Med. Hypotheses 60, 699-701 (that could result from gastric digestion) that corresponds to a PrP fragment that is protease resistant and infective. This remains a postulate.
Other organisms
Transmission of TSEs through ectoparasites has been postulated by Lupi5 . Post et al6 fed larvae of meat eating and myiasis causing flies with brain material from scrapieinfected hamsters. Two days after eating infected material, the larvae showed high amounts of PrPSc by Western blot. In further studies, the inner organs of larvae, which had been fed with scrapie brain, were extracted and fed to hamsters. Six out of eight hamsters developed scrapie. Two out of four hamsters fed on scrapie infected pupae subsequently developed scrapie.
At SEAC36 (September 1996) members considered a paper by Wisniewski et al7 , (data also published subsequently in a paper by Rubenstein et al8 ) who inoculated suspensions of mites from five Icelandic scrapie affected farms into mice, intracerebrally and intraperitoneally. Of 71 mice inoculated, 10 developed clinical TSE, with detection of PrPSc in their brains by Western blot. PrPSc was demonstrated in mite concentrates from one of the farms. The committee also received a presentation on a survey of the prevalence of mites in various cereal products. Some mites were of the same species in the Wisniewski study. SEAC concluded that it was essential that the work on mites be repeated to validate the conclusions. SEAC did not consider that the conclusions raised any public health concerns on mites and TSEs.
Post et al6 found that mites exposed to hamster scrapie and subsequently fed to hamsters did not cause clinical scrapie in the hamsters. Defra has funded two projects to study transmission of TSEs from hay mites, which are now completed.
Project SE1829
Mites of 3 different species that are present on farms in the UK were fed on material contaminated with BSE infected cow brain. The mites were found not to carry sufficient amounts of infection to cause TSE in mouse bioassays. Similarly when the exposed mites were cultured for 2-3 generations no TSE transmission was detectable by mouse bioassay. Attempts to detect conserved DNA sequences from mammalian PrP genes in mite DNA extracts were unsuccessful indicating that mites do not have PrP-like proteins. The results suggest that mites are unlikely to be significant vectors or reservoirs of TSE diseases.
5 Lupi O. (2005) Risk analysis of ectoparasites acting as vectors for chronic wasting disease. Med. Hypotheses 65, 47-54
6 Post K. (1999) Fly larvae and pupae as vectors for scrapie. Lancet 354, 1969-1970
7 Wisniewski H.M., Sigurdarson S., Rubenstein R., Kascsak R.J, Carp R.I. (1996) Mites as vectors for scrapie. Lancet 347, 1114
8 Rubenstein R., Kascsak R.J., Carp, R.I., Papini M., LaFauci G., Sigurarson S., and Wisniewski HM (1998) Potential role of mites as a vector and/or reservoir for scrapie transmission. Alzheimer’s Disease Review 3, 52-56
Project SE1828
Fifteen sheep farms with high incidence scrapie and fifteen farms with low (no reported) incidence scrapie were examined for mite infestation. Overall the results (mite fauna) were closely similar and no clear differences between high and low incidence farms were identified. Samples of mites were cultured to test their ability to carry TSEs. Mice challenged with the mite samples failed to reveal any evidence of infectivity.
Search terms with atypical scrapie on Pubmed:
The following search criteria were used with no result Environmental transmission of atypical scrapie
Atypical scrapie and environment
Atypical scrapie and mites
Atypical scrapie and foxes
Atypical scrapie and birds
Transmission of atypical scrapie by rodents: this revealed use of rodent models rather than transmission by mice in the field.
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