Chronic Wasting Disease: Experimental Chronic Wasting Disease in wild type VM mice

Journal of Veterinary Medical Science Accepted Date: 20 Mar 2013 J-STAGE Advance Published Date: 3 Apr 2013

Virology

Experimental Chronic Wasting Disease in wild type VM mice

RUNNING TITLE: Experimental transmission of CWD to wild mice

MANUSCRIPT TYPE: Note

Yoon-Hee Lee(1)*#, Hyun-Joo Sohn(1)# , Min-Jeong Kim(1), Hyo-Jin Kim(1), Kyung-Je Park(1), Won-Yong Lee(1), Eun-Im Yun(1), Dong-Seob Tark(1), Young-Pyo Choi(1), In- Soo Cho(1) and Aru Balachandran(2)

(1)OIE Reference Laboratory for CWD, Animal, Plant and Fisheries Quarantine and Inspection Agency, Ministry for Food, Agriculture, Forestry and Fisheries, Anyang 1430-757, Republic of Korea

(2)National and OIE Reference Laboratory for Scrapie and CWD, Ottawa Laboratory 1Fallowfield, Ottawa, Canadian Food Inspection Agency, Ottawa, Ontario, Canada

1*Correspondence to Yoon-Hee Lee, Animal, Plant and Fisheries Quarantine and 1Inspection Agency, Ministry for Food, Agriculture, Forestry and Fisheries, Anyang 1430-757, Republic of Korea Virology

2e.mail : lyhee74@korea.kr (Tel: 82-31-467-1879, Fax: 82-31-467-1830) #These two authors contributed equally to this work

Virology

ABSTRACT

Chronic wasting disease (CWD) is a naturally occurring prion disease in North American deer (Odocoileus species), Rocky mountain elk (Cervus elaphus nelsoni) and moose (Alces alces). The disease was first confirmed in the Republic of Korea in 2001, and subsequent cases were diagnosed in 2004, 2005 and 2010. The experimental host range of CWD includes ferrets, several species of voles, white-footed mice, deer mice and Syrian golden hamsters. In addition, CWD was transmitted to the transgenic mouse over-expressing elk or deer prion protein efficiently, but not to wild type mouse. Here, we report the experimental transmission of elk CWD to conventional VM/Dk mice reaching 100% attack rate after second passage. The CWD-prion-affected wild type mice will be a useful model for future CWD studies.

KEY WORDS: CWD, experimental transmission, Republic of Korea, wild type VM mice

https://www.jstage.jst.go.jp/article/jvms/advpub/0/advpub_13-0018/_pdf

Monday, March 18, 2013

PROCEEDINGS ONE HUNDRED AND FIFTEENTH ANNUAL MEETING of the UNITED STATES ANIMAL HEALTH ASSOCIATION September 29 – October 5, 2011

see updated 2012 RESOLUTIONS

http://chronic-wasting-disease.blogspot.com/2013/03/proceedings-one-hundred-and-fifteenth.html

The potential impact of prion diseases on human health was greatly magnified by the recognition that interspecies transfer of BSE to humans by beef ingestion resulted in vCJD. While changes in animal feed constituents and slaughter practices appear to have curtailed vCJD, there is concern that CWD of free-ranging deer and elk in the U.S. might also cross the species barrier. Thus, consuming venison could be a source of human prion disease. Whether BSE and CWD represent interspecies scrapie transfer or are newly arisen prion diseases is unknown. Therefore, the possibility of transmission of prion disease through other food animals cannot be ruled out. There is evidence that vCJD can be transmitted through blood transfusion. There is likely a pool of unknown size of asymptomatic individuals infected with vCJD, and there may be asymptomatic individuals infected with the CWD equivalent. These circumstances represent a potential threat to blood, blood products, and plasma supplies.

http://cdmrp.army.mil/prevfunded/nprp/NPRP_Summit_Final_Report.pdf