Chronic Wasting Disease: TVMDL offers a new, faster CWD testing option

TVMDL offers a new, faster CWD testing option

September 21, 2016 by Shawn Eyre

The Texas A&M Veterinary Medical Diagnostic Laboratory now offers a postmortem Enzyme-linked immunosorbent assay (ELISA) for the detection Chronic Wasting Disease (CWD). This test uses fresh or frozen samples of retropharyngeal lymph node and/or obex and typically provides results in 2-3 days.

TEST: Chronic Wasting Disease (ELISA) SPECIMEN: Retropharyngeal lymph node and/or obex of brain PRICE: $25 for single tissue / $30 for both tissues. Submissions containing both retropharyngeal lymph nodes and obex will have only the lymph nodes tested unless otherwise specified. TURNAROUND: College Station, Tuesday-Friday, results typically in 2-3 business days

A full list of TVMDL’s test offering are available online at tvmdl.tamu.edu, and laboratory experts are available to answer any questions. Call the College Station Laboratory at 979.845.3414.

Click here for the CWD Sampling and Shipping / Submission handout

Filed Under: NewsTagged With: chronic wasting disease, CWD

https://tvmdl.tamu.edu/2016/09/21/tvmdl-offers-new-faster-cwd-testing-option/

Wednesday, September 21, 2016

*** TAHC Passes Authorized Personnel Rule at 396th Commission Meeting “Certified CWD Sample Collector” to “Certified CWD Postmortem Sample Collector” ***

http://chronic-wasting-disease.blogspot.com/2016/09/tahc-passes-authorized-personnel-rule.html

Title: Pathological features of chronic wasting disease in reindeer and demonstration of horizontal transmission

Author

item Moore, Sarah item Kunkle, Robert item West greenlee, Mary item Nicholson, Eric item Richt, Juergen item Hamir, Amirali item Waters, Wade item Greenlee, Justin

Submitted to: Emerging Infectious Diseases Publication Type: Peer reviewed journal Publication Acceptance Date: 8/29/2016 Publication Date: N/A Citation:

Interpretive Summary: Chronic wasting disease (CWD) is a fatal neurodegenerative disease that occurs in farmed and wild cervids (deer and elk) of North America and was recently diagnosed in a single free-ranging reindeer (Rangifer tarandus tarandus) in Norway. CWD is a transmissible spongiform encephalopathy (TSE) that is caused by infectious proteins called prions that are resistant to various methods of decontamination and environmental degradation. Little is known about the susceptibility of or potential for transmission amongst reindeer. In this experiment, we tested the susceptibility of reindeer to CWD from various sources (elk, mule deer, or white-tailed deer) after intracranial inoculation and tested the potential for infected reindeer to transmit to non-inoculated animals by co-housing or housing in adjacent pens. Reindeer were susceptible to CWD from elk, mule deer, or white-tailed deer sources after experimental inoculation. Most importantly, non-inoculated reindeer that were co-housed with infected reindeer or housed in pens adjacent to infected reindeer but without the potential for nose-to-nose contact also developed evidence of CWD infection. This is a major new finding that may have a great impact on the recently diagnosed case of CWD in the only remaining free-ranging reindeer population in Europe as our findings imply that horizontal transmission to other reindeer within that herd has already occurred. Further, this information will help regulatory and wildlife officials developing plans to reduce or eliminate CWD and cervid farmers that want to ensure that their herd remains CWD-free, but were previously unsure of the potential for reindeer to transmit CWD.

Technical Abstract: Chronic wasting disease (CWD) is a naturally-occurring, fatal prion disease of cervids. Reindeer (Rangifer tarandus tarandus) are susceptible to CWD following oral challenge, and CWD was recently reported in a free-ranging reindeer of Norway. Potential contact between CWD-affected cervids and Rangifer species that are free-ranging or co-housed on farms presents a potential risk of CWD transmission. The aims of this study were to 1) investigate the transmission of CWD from white-tailed deer (Odocoileus virginianus; CWDwtd), mule deer (Odocoileus hemionus; CWDmd), or elk (Cervus elaphus nelsoni; CWDelk) to reindeer via the intracranial route, and 2) to assess for direct and indirect horizontal transmission to non-inoculated sentinels. Three groups of 5 reindeer fawns were challenged intracranially with CWDwtd, CWDmd, or CWDelk. Two years after challenge of inoculated reindeer, non-inoculated negative control reindeer were introduced into the same pen as the CWDwtd inoculated reindeer (direct contact; n=4) or into a pen adjacent to the CWDmd inoculated reindeer (indirect contact; n=2). Experimentally inoculated reindeer were allowed to develop clinical disease. At death/euthanasia a complete necropsy examination was performed, including immunohistochemical testing of tissues for disease-associated CWD prion protein (PrPcwd). Intracranially challenged reindeer developed clinical disease from 21 months post-inoculation (months PI). PrPcwd was detected in 5 out of 6 sentinel reindeer although only 2 out of 6 developed clinical disease during the study period (< 57 months PI). We have shown that reindeer are susceptible to CWD from various cervid sources and can transmit CWD to naïve reindeer both directly and indirectly.

Monday, September 05, 2016

*** Pathological features of chronic wasting disease in reindeer and demonstration of horizontal transmission Major Findings for Norway ***

https://www.ars.usda.gov/research/publications/publication/?seqNo115=328261

some of you might be interested with the following science, or not ;

Wednesday, September 7, 2016

*** An assessment of the long-term persistence of prion infectivity in aquatic environments

http://transmissiblespongiformencephalopathy.blogspot.com/2016/09/an-assessment-of-long-term-persistence.html

Friday, September 02, 2016

*** Chronic Wasting Disease Drives Population Decline of White-Tailed Deer

http://chronic-wasting-disease.blogspot.com/2016/09/chronic-wasting-disease-drives.html

Monday, September 05, 2016

Pathological features of chronic wasting disease in reindeer and demonstration of horizontal transmission Major Findings for Norway

http://chronic-wasting-disease.blogspot.com/2016/09/pathological-features-of-chronic.html

Wednesday, August 31, 2016

*** NORWAY CONFIRMS 4TH CASE OF CHRONIC WASTING DISEASE CWD TSE PRION IN SECOND CARIBOU

http://chronic-wasting-disease.blogspot.com/2016/08/norway-confirms-4th-case-of-chronic.html

Wednesday, September 21, 2016

ILLINOIS -- Deer disease CWD meetings set Oct. 18 at the Nash Recreation Center in Oregon, Illinois, and Oct. 19 at the Big Rock Park District Community Building in Big Rock

http://chronic-wasting-disease.blogspot.com/2016/09/illinois-deer-disease-cwd-meetings-set.html

Wednesday, September 21, 2016

Pennsylvania Game commission to present forum on deer chronic wasting disease cwd tse prion

http://chronic-wasting-disease.blogspot.com/2016/09/pennsylvania-game-commission-to-present.html Monday, August 29, 2016

*** NWHC USGS CHRONIC WASTING DISEASE CWD TSE PRION UPDATE

http://chronic-wasting-disease.blogspot.com/2016/08/nwhc-usgs-chronic-wasting-disease-cwd.html

Thursday, August 18, 2016

*** PROCEEDINGS ONE HUNDRED AND Nineteenth ANNUAL MEETING of the USAHA BSE, CWD, SCRAPIE, PORCINE TSE PRION October 22 28, 2015 ***

http://chronic-wasting-disease.blogspot.com/2016/08/proceedings-one-hundred-and-nineteenth.html

Sunday, August 28, 2016

*** TSE PRIONS AKA MAD COW TYPE DISEASE, LIONS AND TIGERS AND BEARS, OH MY! ***

http://transmissiblespongiformencephalopathy.blogspot.com/2016/08/transmissible-spongiform-encephalopathy.html