Tuesday, June 25, 2013

Immunization with a Synthetic Peptide Vaccine Fails to Protect Mule Deer (Odocoileus hemionus) from Chronic Wasting Disease

Immunization with a Synthetic Peptide Vaccine Fails to Protect Mule Deer (Odocoileus hemionus) from Chronic Wasting Disease

 

 

 

John L. Pilon1,7, Jack C. Rhyan2, Lisa L. Wolfe3, Tracy R. Davis3, Matt P. McCollum2, Katherine I. O’Rourke4,6, Terry R. Spraker5, Kurt C. VerCauteren1, Michael W. Miller3, Thomas Gidlewski1, Tracy A. Nichols1, Lowell A. Miller1 and Pauline Nol2,8

 

 

 

+ Author Affiliations

 

 

 

1United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service (APHIS), Wildlife Services (WS), National Wildlife Research Center (NWRC), Fort Collins, Colorado 80521, USA 2USDA, APHIS, Veterinary Services, NWRC, Fort Collins, Colorado 80521, USA 3Colorado Division of Parks and Wildlife, Fort Collins, Colorado 80521, USA 4USDA, Agricultural Research Service, Washington State University, Pullman, Washington 99164, USA 5Department of Pathology and Microbiology, Colorado State University, Fort Collins, Colorado 80523, USA 6Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164, USA ↵8 Corresponding author (email: pauline.nol@aphis.usda.gov) Abstract Chronic wasting disease (CWD) adversely affects both wild and captive cervid populations. A vaccine to prevent CWD would be a highly desirable tool to aid in disease management. To this end, we tested in mule deer a combination of CWD vaccines consisting of cervid prion peptide sequences 168-VDQYNNQNTFVHDC-182 and 145-NDYEDRYYRENMYRYPNQ-164 that had previously been shown to delay onset of clinical disease and increase survival in a mouse-adapted scrapie model. Thirteen captive mule deer (Odocoileus hemionus) were divided into vaccine (n=7) and control groups (n=6), and given prime and boost vaccinations intramuscularly 5 wk apart. Eight weeks postprime (3 wk postboost), all animals were challenged via natural exposure to an environment contaminated with infective CWD prions. Deer were monitored intermittently for prion infection by rectal and tonsil biopsies beginning 275 days postchallenge. All vaccinates responded to both peptide conjugates present in the combination vaccine as measured by enzyme-linked immunosorbent assay. However, all deer eventually became infected regardless of vaccine status.

 

 

 


 

 

 

Monday, June 24, 2013

 

The Effects of Chronic Wasting Disease on the Pennsylvania Cervid Industry Following its Discovery

 


 

 

 

Thursday, June 20, 2013

 

atypical, BSE, CWD, Scrapie, Captive Farmed shooting pens (livestock), Wild Cervids, Rectal Mucosa Biopsy 2012 USAHA Proceedings, and CJD TSE prion Update

 


 

 

 

Tuesday, June 25, 2013

 

Emerging Infectious Diseases (-$2.425 million) This request includes funds to focus on necessary activities for prion disease

 

A FOOLISH MOVE BY THE GOVERNMENT...TSS

 


 

 

 

 

TSS

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