Immunization with a Synthetic Peptide Vaccine Fails to Protect Mule Deer (Odocoileus hemionus) from Chronic Wasting Disease
Immunization with a Synthetic Peptide Vaccine Fails to Protect Mule Deer
(Odocoileus hemionus) from Chronic Wasting Disease
John L. Pilon1,7, Jack C. Rhyan2, Lisa L. Wolfe3, Tracy R. Davis3, Matt P.
McCollum2, Katherine I. O’Rourke4,6, Terry R. Spraker5, Kurt C. VerCauteren1,
Michael W. Miller3, Thomas Gidlewski1, Tracy A. Nichols1, Lowell A. Miller1 and
Pauline Nol2,8
+ Author Affiliations
1United States Department of Agriculture (USDA), Animal and Plant Health
Inspection Service (APHIS), Wildlife Services (WS), National Wildlife Research
Center (NWRC), Fort Collins, Colorado 80521, USA 2USDA, APHIS, Veterinary
Services, NWRC, Fort Collins, Colorado 80521, USA 3Colorado Division of Parks
and Wildlife, Fort Collins, Colorado 80521, USA 4USDA, Agricultural Research
Service, Washington State University, Pullman, Washington 99164, USA 5Department
of Pathology and Microbiology, Colorado State University, Fort Collins, Colorado
80523, USA 6Department of Veterinary Microbiology and Pathology, College of
Veterinary Medicine, Washington State University, Pullman, Washington 99164, USA
↵8 Corresponding author (email: pauline.nol@aphis.usda.gov) Abstract Chronic
wasting disease (CWD) adversely affects both wild and captive cervid
populations. A vaccine to prevent CWD would be a highly desirable tool to aid in
disease management. To this end, we tested in mule deer a combination of CWD
vaccines consisting of cervid prion peptide sequences 168-VDQYNNQNTFVHDC-182 and
145-NDYEDRYYRENMYRYPNQ-164 that had previously been shown to delay onset of
clinical disease and increase survival in a mouse-adapted scrapie model.
Thirteen captive mule deer (Odocoileus hemionus) were divided into vaccine (n=7)
and control groups (n=6), and given prime and boost vaccinations intramuscularly
5 wk apart. Eight weeks postprime (3 wk postboost), all animals were challenged
via natural exposure to an environment contaminated with infective CWD prions.
Deer were monitored intermittently for prion infection by rectal and tonsil
biopsies beginning 275 days postchallenge. All vaccinates responded to both
peptide conjugates present in the combination vaccine as measured by
enzyme-linked immunosorbent assay. However, all deer eventually became infected
regardless of vaccine status.
Monday, June 24, 2013
The Effects of Chronic Wasting Disease on the Pennsylvania Cervid Industry
Following its Discovery
Thursday, June 20, 2013
atypical, BSE, CWD, Scrapie, Captive Farmed shooting pens (livestock), Wild
Cervids, Rectal Mucosa Biopsy 2012 USAHA Proceedings, and CJD TSE prion Update
Tuesday, June 25, 2013
Emerging Infectious Diseases (-$2.425 million) This request includes funds
to focus on necessary activities for prion disease
A FOOLISH MOVE BY THE GOVERNMENT...TSS
TSS
posted by Terry S. Singeltary Sr. at
11:01 AM
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