Prion Disease Detection, PMCA Kinetics, and IgG in Urine from Naturally/Experimentally Infected Scrapie Sheep and Preclinical/Clinical CWD Deer

J. Virol. doi:10.1128/JVI.05111-11 Copyright © 2011,American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. This Article

Prion Disease Detection, PMCA Kinetics, and IgG in Urine from Naturally/Experimentally Infected Scrapie Sheep and Preclinical/Clinical CWD Deer

Richard Rubensteina,*, Binggong Changa, Perry Grayb, Martin Piltchb, Marie S. Bulginc, Sharon Sorensen-Melsonc, and Michael W. Millerd a Departments of Neurology and Physiology/Pharmacology, SUNY Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, NY 11203, USA b Los Alamos National Laboratory, Los Alamos, NM 87545, USA c University of Idaho, Caine Veterinary Teaching and Research Center, 1020 E. Homedale Road, Caldwell, ID 83607, USA d Colorado Division of Wildlife, Wildlife Research Center, 317 West Prospect Road, Fort Collins, CO 80526-2097, USA

* Please send all correspondence to: Dr. Richard Rubenstein, SUNY Downstate Medical Center, Departments of Neurology and Physiology/Pharmacology, Box #1213, 450 Clarkson Avenue, Brooklyn, NY 11203, Tel: 718 270-2019, Fax: 718 270-2459, Email: richard.rubenstein@downstate.edu


ABSTRACT


Prion diseases, also known as transmissible spongiform encephalopathies, are fatal neurodegenerative disorders. Low levels of infectious agent and limited, infrequent success of disease transmissibility and PrPSc detection have been reported with urine from experimentally infected clinical cervids and rodents. We report the detection of prion disease-associated seeding activity (PASA) in urine from naturally and orally infected clinical scrapie sheep and orally infected preclinical and clinical CWD-infected white-tailed deer. This is the first report on PASA detection of PrPSc from the urine of naturally or preclinical prion diseased ovine or cervids. Detection was achieved using the surround optical fiber immunoassay (SOFIA) to measure the products of limited serial protein misfolding cyclic amplification (sPMCA). Conversion of PrPC to PrPSc was not influenced by the presence of poly(A) during sPMCA or by the homogeneity of the PrP genotypes between the PrPC source and urine donor animals. Analysis of the sPMCA-SOFIA data resembled a linear, rather than an exponential, course. Compared to uninfected animals, there was a 2-4 log increase of proteinase K-sensitive, light-chain immunoglobulin (Ig) G fragments in scrapie infected sheep but not in CWD-infected deer. The higher than normal range of IgG levels found in the naturally and experimentally infected clinical scrapie sheep was independent of their genotypes. Although analysis of urine samples throughout the course of infection would be necessary to determine the usefulness of altered IgG levels as a disease biomarker, detection of PrPSc from PASA in urine points to its potential value for ante-mortem diagnosis of prion diseases.


http://jvi.asm.org/cgi/content/short/JVI.05111-11v1?rss=1




Thursday, June 09, 2011

Detection of CWD prions in salivary, urinary, and intestinal tissues of deer: potential mechanisms of prion shedding and transmission

http://chronic-wasting-disease.blogspot.com/2011/06/detection-of-cwd-prions-in-salivary.html





Tuesday, September 02, 2008

Detection of infectious prions in urine (Soto et al Available online 13 August 2008.)

http://chronic-wasting-disease.blogspot.com/2008/09/detection-of-infectious-prions-in-urine.html





CWD, GAME FARMS, BAITING, AND POLITICS


http://chronic-wasting-disease.blogspot.com/2009/01/cwd-game-farms-baiting-and-politics.html




http://chronic-wasting-disease.blogspot.com/2008/08/cwd-feeding-and-baiting-piles.html





2011 AND WE STILL HAVE HUNTERS POURING 100% UNTESTED (potentially CWD infected) URINE ALL OVER THEMSELVES FOR A KILL. ...TSS



Subject: MAD DEER/ELK DISEASE AND POTENTIAL SOURCES

Date: Sat, 25 May 2002 18:41:46 -0700

From: "Terry S. Singeltary Sr."

Reply-To: Bovine Spongiform Encephalopathy

To: BSE-L@uni-karlsruhe.de

now, what about those 'deer scents' of 100% urine', and the prion that is found in urine, why not just pass the prion with the urine to other deer...

Mrs. Doe Pee Doe in Estrus Model FDE1 Mrs. Doe Pee's Doe in Estrus is made from Estrus urine collected at the peak of the rut, blended with Fresh Doe Urine for an extremely effective buck enticer. Use pre-rut before the does come into heat. Use during full rut when bucks are most active. Use during post-rut when bucks are still actively looking for does. 1 oz.

www.gamecalls.net/hunting...lures.html



ELK SCENT/SPRAY BOTTLE

Works anytime of the year *

100 % Cow Elk-in-Heat urine (2oz.) *

Economical - mix with water in spray mist bottle *

Use wind to your advantage

Product Code WP-ESB $9.95

www.elkinc.com/Scent.asp



prions in urine?



DEER & ELK URINE, LURES & SCENT CONTROL DEPARTMENT by MRS.DOE PEE'S Main Index

The Turkey Pro Sez... "Premium, fresh, top-quality, pure 100% undiluted deer lures from Mrs. Doe Pee really work. I won't trust anything else when I'm after big bucks. Sam Collora, owner of the company, proved how well his products work when he bagged this monster buck in 1996.............snip......end........CWD

http://www.turkeyhuntingsecrets.com/store/store-luresandscentcontroldept.htm


snip...end

######## Bovine Spongiform Encephalopathy #########



CDC WARNING ON PRION DISEASE NORTH AMERICA

Thursday, May 26, 2011

Travel History, Hunting, and Venison Consumption Related to Prion Disease Exposure, 2006-2007 FoodNet Population Survey

Journal of the American Dietetic Association Volume 111, Issue 6 , Pages 858-863, June 2011.

http://transmissiblespongiformencephalopathy.blogspot.com/2011/05/travel-history-hunting-and-venison.html





Monday, June 27, 2011

Zoonotic Potential of CWD: Experimental Transmissions to Non-Human Primates

http://chronic-wasting-disease.blogspot.com/2011/06/zoonotic-potential-of-cwd-experimental.html





Saturday, May 14, 2011

Modeling Routes of Chronic Wasting Disease Transmission: Environmental Prion Persistence Promotes Deer Population Decline and Extinction

http://chronic-wasting-disease.blogspot.com/2011/05/modeling-routes-of-chronic-wasting.html





Thursday, April 28, 2011

Chronic Wasting Disease Testing and Prevalence Wisconsin April 2011

http://chronic-wasting-disease.blogspot.com/2011/04/chronic-wasting-disease-testing-and.html





Tuesday, January 25, 2011

Generation of a new form of human PrPSc in vitro by inter-species transmission from cervids prions

http://chronic-wasting-disease.blogspot.com/2011/01/generation-of-new-form-of-human-prpsc.html





Wednesday, April 06, 2011

Presence and Seeding Activity of Pathological Prion Protein (PrPTSE) in Skeletal Muscles of White-Tailed Deer Infected with Chronic Wasting Disease

http://chronic-wasting-disease.blogspot.com/2011/04/presence-and-seeding-activity-of.html





Wednesday, January 5, 2011

ENLARGING SPECTRUM OF PRION-LIKE DISEASES Prusiner Colby et al 2011

Prions

David W. Colby1,* and Stanley B. Prusiner1,2

http://betaamyloidcjd.blogspot.com/2011/01/enlarging-spectrum-of-prion-like.html





UPDATED DATA ON 2ND CWD STRAIN

Wednesday, September 08, 2010

CWD PRION CONGRESS SEPTEMBER 8-11 2010

http://chronic-wasting-disease.blogspot.com/2010/09/cwd-prion-2010.html





Thursday, April 03, 2008

A prion disease of cervids: Chronic wasting disease

2008 1: Vet Res. 2008 Apr 3;39(4):41

A prion disease of cervids: Chronic wasting disease

Sigurdson CJ.

snip...


*** twenty-seven CJD patients who regularly consumed venison were reported to the Surveillance Center***,



snip...

full text ;

http://chronic-wasting-disease.blogspot.com/2008/04/prion-disease-of-cervids-chronic.html





1989

IN CONFIDENCE

Perceptions of unconventional slow virus diseases of animals in the USA

Spraker suggested an interesting explanation for the occurrence of CWD. The deer pens at the Foot Hills Campus were built some 30-40 years ago by a Dr. Bob Davis. At or about that time, allegedly, some scrapie work was conducted at this site. When deer were introduced to the pens they occupied ground that had previously been occupied by sheep. Whether they were scrapie infected sheep or not is unclear.

http://collections.europarchive.org/tna/20080102193705/http://www.bseinquiry.gov.uk/files/mb/m11b/tab01.pdf





http://wildlife.state.co.us/NR/rdonlyres/C82EB818-90C6-4D85-897E-9CE279546CCB/0/JWDEpiCWD.pdf





CHRONIC WASTING DISEASE EXPOSURE RISKS FROM COLORADO DIVISION OF WILDLIFE RESEARCH ACTIVITIES



http://wildlife.state.co.us/NR/rdonlyres/06C89413-E09F-49FB-AEF8-1AFCF4D00C27/0/research_movements.pdf




CJD9/10022

October 1994

Mr R.N. Elmhirst Chairman British Deer Farmers Association Holly Lodge Spencers Lane BerksWell Coventry CV7 7BZ

Dear Mr Elmhirst,

CREUTZFELDT-JAKOB DISEASE (CJD) SURVEILLANCE UNIT REPORT

Thank you for your recent letter concerning the publication of the third annual report from the CJD Surveillance Unit. I am sorry that you are dissatisfied with the way in which this report was published.

The Surveillance Unit is a completely independant outside body and the Department of Health is committed to publishing their reports as soon as they become available. In the circumstances it is not the practice to circulate the report for comment since the findings of the report would not be amended. In future we can ensure that the British Deer Farmers Association receives a copy of the report in advance of publication.

The Chief Medical Officer has undertaken to keep the public fully informed of the results of any research in respect of CJD. This report was entirely the work of the unit and was produced completely independantly of the the Department.

The statistical results reqarding the consumption of venison was put into perspective in the body of the report and was not mentioned at all in the press release. Media attention regarding this report was low key but gave a realistic presentation of the statistical findings of the Unit. This approach to publication was successful in that consumption of venison was highlighted only once by the media ie. in the News at one television proqramme.

I believe that a further statement about the report, or indeed statistical links between CJD and consumption of venison, would increase, and quite possibly give damaging credence, to the whole issue. From the low key media reports of which I am aware it seems unlikely that venison consumption will suffer adversely, if at all.


http://web.archive.org/web/20030511010117/http://www.bseinquiry.gov.uk/files/yb/1994/10/00003001.pdf







TSS