Sunday, August 25, 2013
Prion2013 Chronic Wasting Disease CWD risk factors, humans, domestic cats, 
blood, and mother to offspring transmission 
HD.13: CWD infection in the spleen of humanized transgenic mice 
Liuting Qing and Qingzhong Kong 
Case Western Reserve University; Cleveland, OH USA 
Chronic wasting disease (CWD) is a widespread prion disease in free-ranging 
and captive cervid species in North America, and there is evidence suggesting 
the existence of multiple CWD strains. The susceptibility of human CNS and 
peripheral organs to the various CWD prion strains remains largely unclear. 
Current literature suggests that the classical CWD strain is unlikely to infect 
human brain, but the potential for peripheral infection by CWD in humans is 
unknown. We detected protease-resistant PrpSc in the spleens of a few humanized 
transgenic mice that were intracerebrally inoculated with natural CWD isolates, 
but PrpSc was not detected in the brains of any of the CWD-inoculated mice. Our 
ongoing bioassays in humanized Tg mice indicate that intracerebral challenge 
with such PrpSc-positive humanized mouse spleen already led to prion disease in 
most animals. These results indicate that the CWD prion may have the potential 
to infect human peripheral lymphoid tissues. 
===== 
HD.12: Comparative study of the distribution of the prion protein in the 
squirrel monkey (Saimiri sciureus) following experimental challenge with variant 
and sporadic CJD 
Diane L. Ritchie,1 Paul Brown,2 Susan Gibson,3 Thomas R. Kreil,4 Christian 
Abee3 and James W. Ironside1 
1National CJD Surveillance Unit; Edinburgh, UK; 2Bethesda; Bethesda, MD 
USA; 3Deparment of Comparative Medicine; University of South Alabama; Mobile, AL 
USA; 4Baxter Bioscience; Vienna, Austria 
Introduction, Reports suggest that the number of tissues and organs showing 
the presence of the abnormal prion protein (PrPTSE) in variant CJD (vCJD) 
patients may be greater than previously thought. A limited peripheral 
involvement in some cases of sporadic CJD (sCJD) has also been reported. This 
accumulation of PrPTSE outside the brain has raised concerns about the possible 
iatrogenic transmission risk of vCJD. The squirrel monkey (Saimiri sciureus) has 
been shown to be highly susceptible to experimental challenge with human prion 
disease. Neuropathological and biochemical analyses of CNS tissue have shown 
that sCJD and vCJD can be distinguished in the squirrel monkey and that many of 
the strain characteristics that define these agents are conserved after 
transmission. Following on from these initial studies, immunohistochemistry and 
western blot analysis were performed on a wide range of peripheral tissues 
including, lymphoreticular tissues and peripheral neural tissue to establish the 
full-body distribution of PrPTSE in this primate animal model. 
Materials and Methods. Brain homogenates from sCJD or vCJD patients were 
inoculated into the frontal cortex of squirrel monkeys. Animals were kept under 
constant clinical surveillance. At post-mortem, formalin fixed CNS tissue and a 
wide range of peripheral tissues were taken for immunohistochemical analysis 
together with frozen tissues taken for the biochemical detection of PrPTSE. 
Results. Immunohistochemical analysis showed no evidence of PrPTSE 
deposition in peripheral tissues in either variant or sporadic CJD-infected 
animals. However, western blot assays detected PrPTSE in the spleen of a 
proportion of the vCJD- infected animals. The PrPTSE isotype resembled that 
detected in CNS tissue from the vCJD- infected animals and from human vCJD 
cases. ***In addition, western blot analysis detected PrPTSE in the spleen of a 
single animal following challenge with sporadic CJD. The PrPTSE type in this 
animal resembled that found in CNS tissue from the same animal, with a PrPTSE 
type similar to that found in human sCJD type 1 cases. 
Conclusion. This study confirms the accumulation of PrPTSE in the CNS and 
spleen of a proportion of squirrel monkeys infected intra-cerebrally with human 
vCJD. Furthermore, this study extends the evidence that there may be a 
peripheral involvement in some cases of sCJD. PrPTSE typing confirms the 
conservation of PrPTSE type on transmission to the squirrel monkey and suggests 
that there are no tissue-specific adaptations in the biochemical phenotype of 
the agent strain following primate-to-primate transmission. 
===== 
Oral.15: Molecular barriers to zoonotic prion transmission: Comparison of 
the ability of sheep, cattle and deer prion disease isolates to convert normal 
human prion protein to its pathological isoform in a cell-free system 
Marcelo A.Barria,1 Aru Balachandran,2 Masanori Morita,3 Tetsuyuki 
Kitamoto,4 Rona Barron,5 Jean Manson,5 Richard Kniqht,1 James W. lronside1 and 
Mark W. Head1 
1National CJD Research and Surveillance Unit; Centre for Clinical Brain 
Sciences; School of Clinical Sciences; The University of Edinburgh; Edinburgh, 
UK; 2National and OIE Reference Laboratory for Scrapie and CWD; Canadian Food 
Inspection Agency; Ottawa Laboratory; Fallowfield. ON Canada; 3Infectious 
Pathogen Research Section; Central Research Laboratory; Japan Blood Products 
Organization; Kobe, Japan; 4Department of Neurological Science; Tohoku 
University Graduate School of Medicine; Sendai. Japan; 5Neurobiology Division; 
The Roslin Institute and R(D)SVS; University of Edinburgh; Easter Bush; 
Midlothian; Edinburgh, UK 
Background. Bovine spongiform encephalopathy (BSE) is a known zoonotic 
prion disease, resulting in variant Creurzfeldt- Jakob disease (vCJD) in humans. 
In contrast, classical scrapie in sheep is thought to offer little or no danger 
to human health. However, a widening range of prion diseases have been 
recognized in cattle, sheep and deer. The risks posed by individual animal prion 
diseases to human health cannot be determined a priori and are difficult to 
assess empirically. The fundamemal event in prion disease pathogenesis is 
thought to be the seeded conversion of normal prion protein (PrPC) to its 
pathological isoform (PrPSc). Here we report the use of a rapid molecular 
conversion assay to test whether brain specimens from different animal prion 
diseases are capable of seeding the conversion of human PrPC ro PrPSc. 
Material and Methods. Classical BSE (C-type BSE), H-type BSE, L-type BSE, 
classical scrapie, atypical scrapie, chronic wasting disease and vCJD brain 
homogenates were tested for their ability to seed conversion of human PrPC to 
PrPSc in protein misfolding cyclic amplification (PMCA) reactions. Newly formed 
human PrPSc was detected by protease digestion and western blotting using the 
antibody 3F4. 
Results. C-type BSE and vCJD were found to efficiently convert PrPC to 
PrPSc. Scrapie failed to convert human PrPC to PrPSc. Of the other animal prion 
diseases tested only chronic wasting disease appeared to have the capability ro 
convert human PrPC to PrPSc. The results were consistent whether the human PrPC 
came from human brain, humanised transgenic mouse brain or from cultured human 
cells and the effect was more pronounced for PrPC with methionine at codon 129 
compared with that with valine. 
Conclusion. Our results show that none of the tested animal prion disease 
isolates are as efficient as C-type BSE and vCJD in converting human prion 
protein in this in vitro assay. However, they also show that there is no 
absolute barrier ro conversion of human prion protein in the case of chronic 
wasting disease. 
===== 
Invited.16: Studies of chronic wasting disease transmission in cervid and 
non-cervid species 
Edward A, Hoover,1 Candace K. Mathiason,1 Davin M. Henderson,1 Nicholas J. 
Haley,1 Davis M. Seelig,1 Nathaniel D. Denkers,1 Amy V. Nalls,1 Mark D. Zabe,1 
Glenn C. Telling,1 Fernando Goni2 and Thomas Wisniewski,2 
1Prion Research Center; Colorado State University; Fort Collins, CO USA; 
2New York University School of Medicine; New York, NY USA 
How and why some misfolded proteins become horizontally transmitted agents 
and occasionally cross species barriers are issues fundamental to understanding 
prion disease. Chronic wasting disease (CWD) of cervids is perhaps a prototype 
of horizontal prion transmission, encompassing efficient mucosal uptake, 
lymphoid amplification, neuroinvasion, peripheralization, and dissemination via 
mucosal excretion. Efficient mucosal transmission of CWD in deer has been 
demonstrated by oral, nasal, aerosol, and indirect contact exposure. In 
addition, other studies (Mathiason CK, et al.) reported at the symposium support 
a significant role for pre- and/or postnatal transmission of CWD from doe to 
offspring. Accumulating, yet still incomplete, evidence also suggests that the 
period of relatively covert CWD infection may be longer than originally thought. 
Given the above, minimally invasive sensitive assays based on body fluids from 
live animals would aid substantially in understanding the biology of CWD. We 
have been applying seeded realtirne quaking-induced amplification of recombinant 
PrP substrates (i.e., RT-QuIC methodology) to: (1) investigate antemortem CWD 
detection, and (2) model PrP-based species barriers and trans-species 
adaptation-topics we previously explored using sPMCA and in vivo bioassays. At 
this symposium, we report sensitive and specific detection CWD prions in saliva, 
urine, blood (Mathiason lab), and rectal and pharyngeal lymph node samples 
(Haley NJ, et al.) from pre-symptomatic and symptomatic experimentally and 
naturally exposed deer. Other ongoing studies are employing RT-QuIC methodology 
to model amplification barriers among CWD, FSE, BSE, and CJD prions using 
cervine, feline, bovine, human, and promiscuous rPrP substrates and the above 
species prion seeds, cellular co-factors, and transgenic mice. Finally, in 
collaboration with the Wisniewski laboratory, we are conducting of experimental 
CWD vaccination studies in deer employing oral administration of an attenuated 
Salmonella vector expressing cervid PrP epitopes. 
===== 
AD.06: Detecting prions in the brain and blood of TSE-infected deer and 
hamsters 
Alan Elder,1 Davin Henderson,1 Anca Selariu,1 Amy Nalls,1 Byron Caughey,2 
Richard Bessen,1 Jason Bartz3 and Candace Mathiason1 
1Colorado State University; Fort Collins, CO USA; 2NIH Rocky Mountain 
Laboratories; Hamilton, MT USA; 3Creighton University; Omaha, NE USA 
While large quantities of protease resistant prion protein (PrPres) can be 
demonstrated by western blot or IHC in lymphoid biopsies or post-mortem brain 
tissues harvested from prion-infected animals, these conventional assays are 
less reliable as means to detect the small quantities of prions thought to be 
present in bodily fluids or associated with early and asymptomatic phases of TSE 
disease. The Real Time-Quaking Induced Conversion (RT-QuIC) assay is capable of 
detecting prions at concentrations below the level of sensitivity of 
conventional assays and provides a real-time fluorescent readout negating the 
use of proteases. We have made modifications to the RT-QuIC assay to utilize it 
for the detection of PrPres in brain and blood harvested from various species 
infected with prions. In this study, we analyzed CWD-infected deer and 
CWD/TME-infected hamster whole blood to determine the effect of: 
(1) various anticoagulants, 
(2) freezing and 
(3) NaPTA precipitation. 
Brain tissue and blood collected from naive deer and hamsters served as 
negative controls. 
We were able to demonstrate amplifiable prions in 
(1) brain and blood samples harvested from CWD/TME-infected animals, 
(2) heparinized blood, 
(3) frozen vs. fresh blood and 
(4) NaPTA treated samples. 
The RT-QuIC assay is able to detect PrPres in various species of animals 
and shows promise as an antemortem diagnostic tool for blood-borne TSEs. 
===== 
Oral.08: Mother to offspring transmission of chronic wasting disease in 
Reeve's Muntjac deer 
Amy Nalls,1 Erin McNulty,1 Jenny Powers,2 Davis Seelig,1 Clare Hoover,1 
Nicholas Haley,1 Jeanette Hayes-Klug,1 Kelly Anderson,1 Paula Stewart,3 Wilfred 
Goldmann,3 Edward A. Hoover1 and Candace K. Mathiason1 
1Colorado State University; Fort Collins, CO USA; 2National Park Service; 
Fort Collins, CO USA; 3The Roslin Institute and Royal School of Veterinary 
Studies; Edinburgh, UK 
To investigate the role mother to offspring transmission plays in chronic 
wasting disease (CWD), we have developed a cervid model employing the Reeve's 
muntjac deer (Muntiacus reevesi). Eight muntjac doe were orally inoculated with 
CWD and tested PrPCWD lymphoid positive by 4 mo post infection. Fourteen fawns 
were born to these eight CWD-infected doe-3 were born viable, 6 were born 
non-viable and 5 were harvested as fetuses from early or end-stage CWD-infected 
doe. All three viable fawns have demonstrated CWD IHC lymphoid biopsy positivity 
between 43 d post birth and 11 mo post birth. Two of these three CWD positive 
viable offspring have developed clinical signs consistent with TSE disease 
(28-33 mo post birth). Moreover, CWD prions have been detected by sPMCA in 11 of 
16 tissues harvested from 6 full-term non-viable fawns and in 7 of 11 fetal 
tissues harvested in utero from the second and third trimester fetuses. 
Additional tissues and pregnancy related fluids from doe and offspring are being 
analyzed for CWD prions. In summary, using the muntjac deer model we have 
demonstrated CWD clinical disease in offspring born to CWD-infected doe, and in 
utero transmission of CWD from mother to offspring. These studies provide basis 
to further investigate the mechanisms of maternal transfer of prions. 
===== 
AD.63: Susceptibility of domestic cats to chronic wasting disease 
Amy V.Nalls,1 Candace Mathiason,1 Davis Seelig,2 Susan Kraft,1 Kevin 
Carnes,1 Kelly Anderson,1 Jeanette Hayes-Klug1 and Edward A. Hoover1 
1Colorado State University; Fort Collins, CO USA; 2University of Minnesota; 
Saint Paul, MN USA 
Domestic and nondomestic cats have been shown to be susceptible to feline 
spongiform encephalopathy (FSE), almost certainly caused by consumption of 
bovine spongiform encephalopathy (BSE)-contaminated meat. Because domestic and 
free-ranging nondomestic felids scavenge cervid carcasses, including those in 
areas affected by chronic wasting disease (CWD), we evaluated the susceptibility 
of the domestic cat (Felis catus) to CWD infection experimentally. Cohorts of 5 
cats each were inoculated either intracerebrally (IC) or orally (PO) with 
CWD-infected deer brain. At 40 and 42 mo post-inoculation, two IC-inoculated 
cats developed signs consistent with prion disease, including a stilted gait, 
weight loss, anorexia, polydipsia, patterned motor behaviors, head and tail 
tremors, and ataxia, and progressed to terminal disease within 5 mo. Brains from 
these two cats were pooled and inoculated into cohorts of cats by IC, PO, and 
intraperitoneal and subcutaneous (IP/SC) routes. Upon subpassage, feline-adapted 
CWD (FelCWD) was transmitted to all IC-inoculated cats with a decreased 
incubation period of 23 to 27 mo. FelCWD was detected in the brains of all the 
symptomatic cats by western blotting and immunohistochemistry and abnormalities 
were seen in magnetic resonance imaging, including multifocal T2 fluid 
attenuated inversion recovery (FLAIR) signal hyper-intensities, ventricular size 
increases, prominent sulci, and white matter tract cavitation. Currently, 3 of 4 
IP/SQ and 2 of 4 PO inoculared cats have developed abnormal behavior patterns 
consistent with the early stage of feline CWD. These results demonstrate that 
CWD can be transmitted and adapted to the domestic cat, thus raising the issue 
of potential cervid-to- feline transmission in nature. 
Sunday, July 21, 2013 
*** As Chronic Wasting Disease CWD rises in deer herd, what about risk for 
humans? 
> sCJDMM1-2 should be considered as a separate entity at this time. 
> All of the Heidenhain variants were of the methionine/ methionine type 
1 molecular subtype. 
PO-081: Chronic wasting disease in the cat— Similarities to feline 
spongiform encephalopathy (FSE) 
PO-081: Chronic wasting disease in the cat— Similarities to feline 
spongiform encephalopathy (FSE) 
Thursday, May 31, 2012 
CHRONIC WASTING DISEASE CWD PRION2012 Aerosol, Inhalation transmission, 
Scrapie, cats, species barrier, burial, and more 
Monday, August 8, 2011 
Susceptibility of Domestic Cats to CWD Infection 
Enforcement Report - Week of February 20, 2013
Product Detail Product Description Regular Chicken 50# 
Ingredients: Corn, Wheat, Oats, Oyster shells, Medium Grit, CCC, ADS, Plant 
Protein Products, Animal Protein Products, Processed Grain By-Products, Roughage 
Products, Animal Fat procession with DHA, etc 
Recall Number V-137-2013 Classification Class III Code Info 8/6/2012 
Product Distributed Qty 5400lbs (50lb bags) 
Reason For Recall During an FDA sample collection, the firms 50# Regular 
Chicken Feed was found to contain mammalian protein. The label does not contain 
the warning statement. 
Event Detail Event Id 63743 Product Type Veterinary Status Terminated 
Recalling Firm Cohoons Elevator Inc. City Midland State MI Country US Voluntary 
/ Mandated Voluntary: Firm Initiated Recall Initiation Date 2012-11-21 Initial 
Firm Notification of Consignee or Public Other Distribution Pattern Midland MI 
area only. 
Subject: DOCKET-- 03D-0186 -- FDA Issues Draft Guidance on Use of Material 
From Deer and Elk in Animal Feed; Availability
Date: Fri, 16 May 2003 11:47:37 –0500
From: "Terry S. Singeltary Sr."
To: fdadockets@oc.fda.gov 
Tuesday, June 11, 2013 
Weld County Bi-Products dba Fort Morgan Pet Foods 6/1/12 significant 
deviations from requirements in FDA regulations that are intended to reduce the 
risk of bovine spongiform encephalopathy (BSE) within the United States
Thursday, June 6, 2013 
BSE TSE PRION USDA FDA MAD COW FEED COMPLIANCE REPORT and NAI, OAI, and VAI 
ratings as at June 5, 2013 
 Greetings, 
since our fine federal friends have decided not to give out any more 
reports on the USA breaches of the feed ban and surveillance etc. for the BSE 
TSE prion mad cow type disease in the USDA livestock, I thought I might attempt 
it. I swear, I just don’t understand the logic of the SSS policy, and that 
includes all of it. I assure you, it would be much easier, and probably better 
for the FDA and the USDA INC., if they would simply put some kind of report out 
for Pete’s sake, instead of me doing it after I get mad, because I am going to 
put it all out there. the truth. 
PLEASE BE ADVISED, any breach of any of the above classifications OAI, VAI, 
RTS, CAN lead to breaches into the feed BSE TSE prion protocols, and CAN lead to 
the eventual suspect tainted feed reaching livestock. please, if any USDA 
official out there disputes this, please explain then how they could not. 
paperwork errors can eventually lead to breaches of the BSE TSE prion mad cow 
feed ban reaching livestock, or contamination and exposure there from, as well. 
I would sure like to see the full reports of just these ; 
 4018 CHI-DO 3007091297 Rancho Cantera 2866 N Sunnyside Rd Kent IL 
61044-9605 OPR FR, OF HP 11/26/2012 OAI Y 
9367 3008575486 Rocky Ford Pet Foods 21693 Highway 50 East Rocky Ford CO 
81067 OPR RE, TH HP 2/27/2013 OAI N 
9446 DEN-DO 1713202 Weld County Bi Products, Inc. 1138 N 11th Ave Greeley 
CO 80631-9501 OPR RE, TH HP 10/12/2012 OAI N 
9447 DEN-DO 3002857110 Weld County Bi-Products dba Fort Morgan Pet Foods 
13553 County Road 19 Fort Morgan CO 80701-7506 OPR RE HP 12/7/2011 OAI N 
see full list of the fda mad cow bse feed follies, toward the bottom, after 
a short brief update on the mad cow bse follies, and our good friend Lester 
Crawford that was at the FDA. 
ALSO, I would kindly like to comment on this FDA BSE/Ruminant Feed 
Inspections Firms Inventory (excel format)4 format, for reporting these breaches 
of BSE TSE prion protocols, from the extensive mad cow feed ban warning letters 
the fda use to put out for each violations. simply put, this excel format sucks, 
and the FDA et al intentionally made it this difficult to follow the usda fda 
mad cow follies. this is an intentional format to make it as difficult as 
possible to follow these breaches of the mad cow TSE prion safety feed 
protocols. to have absolutely no chronological or numerical order, and to format 
such violations in a way that they are almost impossible to find, says a lot 
about just how far the FDA and our fine federal friends will go through to hide 
these continued violations of the BSE TSE prion mad cow feed ban, and any 
breaches of protocols there from. once again, the wolf guarding the henhouse $$$ 
 NAI = NO ACTION INDICATED
OAI = OFFICIAL ACTION INDICATED
VAI = VOLUNTARY ACTION INDICATED
RTS = REFERRED TO STATE 
 Inspections conducted by State and FDA investigators are classified to 
reflect the compliance status at the time of the inspection, based upon whether 
objectionable conditions were documented. Based on the conditions found, 
inspection results are recorded in one of three classifications: 
OAI (Official Action Indicated) when inspectors find significant 
objectionable conditions or practices and believe that regulatory sanctions are 
warranted to address the establishment’s lack of compliance with the regulation. 
An example of an OAI classification would be findings of manufacturing 
procedures insufficient to ensure that ruminant feed is not contaminated with 
prohibited material. Inspectors will promptly re-inspect facilities classified 
OAI after regulatory sanctions have been applied to determine whether the 
corrective actions are adequate to address the objectionable conditions. 
VAI (Voluntary Action Indicated) when inspectors find objectionable 
conditions or practices that do not meet the threshold of regulatory 
significance, but warrant an advisory to inform the establishment that 
inspectors found conditions or practices that should be voluntarily corrected. 
VAI violations are typically technical violations of the 1997 BSE Feed Rule. 
These violations include minor recordkeeping lapses or conditions involving 
non-ruminant feeds. 
NAI (No Action Indicated) when inspectors find no objectionable conditions 
or practices or, if they find objectionable conditions, those conditions are of 
a minor nature and do not justify further actions. 
when sound science was bought off by junk science, in regards to the BSE 
TSE prion mad cow type disease, by the USDA, CFIA, WHO, OIE, et al. $$$ 
when the infamous, and fraudulently USDA, FSIS, APHIS, FDA, gold card was 
taken away that infamous day in December of 2003, all cards were off the table, 
it was time to change the science, and change they did. ...tss 
 snip. ...please see full text ; 
Thursday, June 6, 2013 
BSE TSE PRION USDA FDA MAD COW FEED COMPLIANCE REPORT and NAI, OAI, and VAI 
ratings as at June 5, 2013 
Friday, July 19, 2013 
PART 589 -- SUBSTANCES PROHIBITED FROM USE IN ANIMAL FOOD OR FEED Revised 
as of April 1, 2013 50# Regular Chicken Feed was found to contain mammalian 
protein label does not contain the warning statement 
IN A NUT SHELL ; 
(Adopted by the International Committee of the OIE on 23 May 2006) 
11. Information published by the OIE is derived from appropriate 
declarations made by the official Veterinary Services of Member Countries. The 
OIE is not responsible for inaccurate publication of country disease status 
based on inaccurate information or changes in epidemiological status or other 
significant events that were not promptly reported to the Central Bureau, 
Thursday, May 30, 2013 
World Organization for Animal Health (OIE) has upgraded the United States' 
risk classification for mad cow disease to "negligible" from "controlled", and 
risk further exposing the globe to the TSE prion mad cow type disease 
U.S. gets top mad-cow rating from international group and risk further 
exposing the globe to the TSE prion mad cow type disease 
Tuesday, July 2, 2013 
APHIS USDA Administrator Message to Stakeholders: Agency Vision and Goals 
Eliminating ALL remaining BSE barriers to export market
Saturday, July 6, 2013 
Small Ruminant Nor98 Prions Share Biochemical Features with Human 
Gerstmann-Sträussler-Scheinker Disease and Variably Protease-Sensitive 
Prionopathy 
Research Article 
Sunday, August 11, 2013 
Development of an oral vaccine for chronic wasting disease 
Thursday, August 08, 2013 
Characterization of the first case of naturally occurring chronic wasting 
disease in a captive red deer (Cervus elaphus) in North America
Friday, August 09, 2013 
CWD TSE prion, plants, vegetables, and the potential for environmental 
contamination
Sunday, June 09, 2013 
Missouri House forms 13-member Interim Committee on the Cause and Spread of 
Chronic Wasting Disease CWD 
Wednesday, August 21, 2013 
IOWA DNR EMERGENCY CONSENT ORDER IN THE MATTER OF TOM & LINDA BRAKKE 
D/B/A PINE RIDGE HUNTING LODGE UPDATE AUGUST 21, 2013 
Friday, August 16, 2013 
Creutzfeldt-Jakob disease (CJD) biannual update August 2013 U.K. and 
Contaminated blood products induce a highly atypical prion disease devoid of 
PrPres in primates 
Sunday, August 11, 2013 
Creutzfeldt-Jakob Disease CJD cases rising North America updated report 
August 2013 
Creutzfeldt-Jakob Disease CJD cases rising North America with Canada seeing 
an extreme increase of 48% between 2008 and 2010 
kind regards, terry 
Wednesday, August 21, 2013
IOWA DNR EMERGENCY CONSENT ORDER IN THE MATTER OF TOM & LINDA BRAKKE D/B/A PINE RIDGE HUNTING LODGE UPDATE AUGUST 21, 2013
IOWA 
DNR EMERGENCY CONSENT ORDER IN THE MATTER OF TOM & LINDA BRAKKE D/B/A PINE 
RIDGE HUNTING LODGE UPDATE AUGUST 21, 2013
IOWA 
DEPARTMENT OF NATURAL RESOURCES EMERGENCY CONSENT ORDER
IN THE 
MATTER OF: 
TOM & 
RHONDA BRAKKE D/B/A PINE RIDGE HUNTING LODGE Davis County, Iowa
EMERGENCY 
CONSENT ORDER NO. 2013-HP- 
TO: Tom and 
Rhonda Brakke, Owners of Pine Ridge Hunting Lodge 22529 Balsam Ave. Clear Lake, 
IA 50428
1. 
SUMMARY
This 
Emergency Consent Order is entered into between the Director of the lowe 
Department of Naturel Resources (“DNR”) and Tom and Rhonda Brakke D/B/A Pine 
Ridge Hunting Lodge (“the Brakkes”) related to violations alleged by the DNR to 
have occurred at the premises they previously managed as a hunting preserve, 
Pine Ridge Hunting Lodge (“Pine Ridge”). This Emergency Consent Order is entered 
into between the parties for the purpose of settling the issues which were the 
subject of a hearing for a stay of the Emergency Order issued by the DNR on June 
5, 2013 ("Emergency Order”).
This 
Emergency Consent Order supersedes the Emergency Order issued on June 6, 2013 
with respect to sections V.5 and V.6 only and only to the extent the terms here 
in are inconsistent with the terms of sections V.5 and V.6 of such Emergency 
Order. Nothing herein shall be construed in any way as an admission of any 
issues or liability by any of the parties to this Emergency Consent Order. 
Questions 
regarding this Emergency Consent Order should be directed to:
Relating to 
technical requirements (DNR): Dr. Dale Garner, Wildlife Bureau Chief Iowa 
Department of Natural Resources 502 East Ninth Street Des Moines, Iowa 50319 
Phone: 515-281-6156 
Relating to 
legal requirements (DNR): Kelley Myers, Attorney for the DNR Iowa Department of 
Natural Resources 502 East Ninth Street Des Moines, iowa 50319 Phone: 
515-281-5534
Angie 
Bruce, Wildlife Bureau Executive Officer Iowa Department of Natural Resources 502 
East Ninth Street Des Moines, Iowa 50319 Phone: 515-281-8070
Relating to 
technical requirements (Brakkes): Relating to legal requirements (Brakkes): 
--------------------------------------------------------------------------------
Page 2 
lOWA 
DEPARTMENT OF NATURAL RESOURCES EMERGENCY CONSENT ORDER ISSUED TO: TOM AND 
RHONDA BRAKKE D/B/A PlNE RIDGE HUNTlNG PRESERVE
Tom Brakke 
22529 Balsam Avenue Clear Lake, IA 50428 Phone: (641)425-2095 
Rebecca A. 
Bromrnel BrownWinick 666 Grand Avenue, Suite 2000 Des Moines, IA 50309 Phone: 
(515)242-2452
ll. 
PROCEDURAL BACKGROUND
1. DNR 
issued an Emergency Order on June 6, 2013, which required the Brakkes to comply 
with six separate provisions.
2. - Upon 
issuance of the Emergency Order, DNR requested the Iowa Department of 
Inspections and Appeals to set the case for a contested case hearing consistent 
with Iowa Code chapter 17A and 561 lAC chapter 7.
3. The 
Brakkes, through their counsel, appealed the Emergency Order on June 25, 2013. 
The Brakkes, through their counsel, flied a Motion for Stay on June 27, 2013, 
requesting the administrative law judge stay the Emergency Order.
4. The 
presiding administrative law judge, Judge Heather Palmer, issued the Notice for 
Hearing on June 18, 2013. 
5. The 
parties, in prehearing conference with the judge, agreed that the hearing would 
be treated as a hearing on a motion for a stay, consistent with 561 lAC section 
7.18(5).
6. The 
parties agreed to settle prior to the judge deciding whether to issue a stay 
ofthe Emergency Order. This Emergency Consent Order includes the terms of that 
settlement.
III. 
ORDER
THEREFORE, 
DNR orders and the Brakkes agree, without admitting any fault or any liability, 
to do the following:
1. The 
parties agree that an electrified fence just inside the existing high perimeter 
fence shall be restored or reconstructed on the following terms:
a. Electric 
Fence. The Brakkes shall maintain such electrified fence until such time as 
described in paragraph b below. The following terms shall apply to the electric 
fence:
i. 
Initiation of the construction and reconstruction efforts must commence by July 
10, 2013, and be completed by July 22, 2013 unless unforeseen conditions are 
encountered or extenuating circumstances arise.
ii. The 
Brakkes and DNR shall split the cost of the materials for and/or installation of 
the fence 50-50. DNR intends to provide its staff for labor 
-------------------------------------------------------------------------------- 
Page 3 
IOWA 
DEPARTMENT OF NATURAL RESOURCES EMERGENCY CONSENT ORDER ISSUED TO: TOM AND 
RHONDA BRAKKE D/B/A PINE RIDGE HUNTING PRESERVE
and such 
labor costs shall be the responsibility of DNR and not included in the costs to 
be divided with the Brakkes.
iii. The 
fence shall be installed in such a manner as to ensure its effectiveness but 
also ease its maintenance. This may require, among other features, attachment to 
existing perimeter fencing so that the electrified portions come up from the 
ground at an approximate 45 degree angle. Regardless of the design, the fence 
must include an electrified wire every linear foot for at least three feet. The 
final design, which should be consistent with this section, shall be subject to 
the approval of at least one of the DNR’s biologists identified in paragraph c 
below and the Brakkes. The fence should be designed and installed in a manner 
that the 50 percent amount to be paid by the Brakkes for such fence does not 
exceed the amount the Brakkes paid pursuant to the September 7, 2012 Agreement 
for Chronic Wasting Disease Recovery Plan at Pine Ridge Hunting Lodge, unless 
otherwise agreed to by the parties. 
iv. After 
construction is complete, the Brakkes shall be solely responsible for all fence 
repairs, maintenance and associated costs, except as stated in paragraph 
3(a)(vii) below.
v. One of 
the DNR Wildlife biologists identified in paragraph c shall conduct a weekly 
internal perimeter fence inspection and shall note all reasonable repairs that 
must be made to make and keep the fence functional. All necessary repairs 
identified by the biologist shall be submitted to the Brakkes in writing and 
completed within 24 hours from the date of the submission, unless such repairs 
cannot be reasonably completed within such time but provided such repairs are 
completed within a reasonable time thereafter. 
vi. One of 
the DNR Wildlife biologists identified in paragraph c shall conduct an internal 
perimeter fence inspection at the washout locations along the perimeter of Pine 
Ridge within one day of a significant rain event and shall note all reasonable 
repairs that must be made to make and keep the fence functional. All necessary 
repairs identified by the biologist shall be submitted to the Brakkes in writing 
and completed within 24 hours of the date of the submission, unless such repairs 
cannot be reasonably completed within such time but provided such repairs are 
completed within are reasonable time thereafter. 
vii. If 
during their inspection, one of the biologist observes a breach or other damage 
to either the perimeter or electric fence that requires immediate action to 
prevent animal release or harm, DNR shall repair such damage on the spot and at 
its own expense. The biologist shall notify the Brakkes of such repairs and 
direct them to make additional repairs if needed. 
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Page 4 
IOWA 
DEPARTMENT OF NATURAL RESOURCES EMERGENCY CONSENT ORDER ISSUED TO: TOM AND 
RHONDA BRAKKE D/B/A PINE RIDGE HUNTING PRESERVE 
viii. At no 
time during the time in which the electric fence is required shall the DNR 
wildlife biologists identified in paragraph c enter Pine Ridge without first 
providing notice to the Brakkes.
ix. The 
electric fence requirements set forth herein shall terminate once depopulation, 
as set forth below, is completed and such depopulation is confirmed by 
DNR.
b. 
Depopulation. The Brakkes shall depopulate the wild deer on Pine Ridge or shall 
authorize one of the DNR Wildlife biologists identified in paragraph c, to 
conduct such activities, as soon as is practicable or feasible given weather and 
terrain conditions. The following conditions shall apply:
i. The 
Brakkes may destroy the deer through any lawful means or may rely on the DNR 
Wildlife Biologists identified in paragraph c below to conduct such 
depopulation.
ii. 
Depopulation activities shall occur until the DNR Wildlife biologists conclude 
that all wild deer on Pine Ridge are destroyed or are likely destroyed. Such 
judgment shall be based on the observation and professional judgment of the 
biologist. To that end, the biologist must be provided with reasonable access to 
Pine Ridge to make such judgment.
iii. 
Depopulation activities must occur prior to the end of the 2013-2014 hunting 
season.
iv. Any 
deer destroyed pursuant to this Emergency Consent Order shall be immediately 
provided to or made available to DNR staff identified in paragraph c in order to 
ensure that testing and disposal can occur. DNR shall collect requisite samples 
and submit them for CWD testing. DNR shall share all results related to deer 
killed-on Pine Ridge with the Brakkes upon receipt of the same. DNR shall be 
responsible for all testing and disposal costs related to these efforts.
c. DNR 
Wildlife Staff. The Brakkes shall refer all communications related to fencing 
orde population to the following DNR Wildlife Staff: Dr. Dale Garner, Wildlife 
Bureau Chief, Angi Bruce, Executive Officer 3; Bill Ohde, Wiidlife District 
Supervisor; Keith Wilcox, Natural Resources Technician 2; Lincoln Utt, Natural 
Resources Technician 1; and Darwin Emmons, Natural Resources Technician 1. The 
Brakkes may suggest additions to this list during the term of this Emergency 
Consent Order with such request being made, in writing or email, to Dr. Dale 
Garner.
2. The 
Brakkes shall not be required to submit an operational plan, as described in 
section V.6 of the Emergency Order, pending a hearing on the merits or 
resolution of this case.
IV. 
DISPOSITION OF MOTION FOR STAY 
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Page 5 
IOWA 
DEPARTMENT OF NATURAL RESOURCES EMERGENCY CONSENT ORDER ISSUED TO: TOM AND 
RHONDA BRAKKE D/B/A PINE RIDGE HUNTING PRESERVE
This 
Emergency Consent Order resolves the Motion for Stay filed by the Brakkes on 
June 27, 2013. DNR acknowledges the Brakkes have appealed the Emergency Order 
and such portions that are subject to their appeal may be heard in the hearing 
on the merits that is tentatively set for November of 2013 regardless of this 
Emergency Consent Order.
V. 
NONCOMPLIANCE
Failure to 
knowingly comply with this Emergency Consent Order may result in referral of 
this matter to the Attorney General or any other appropriate legal authority for 
relief as allowed by law. 
CHUCK GIPP, 
DIRECTOR Iowa Department of Natural Resources
Dated this 
3rd day of July, 2013
TOM BRAKKE, 
CO-OWNER Pine Ridge Hunting Lodge
Dated this 
3 day of July, 2013
CC: Kelley 
Myers, Rebecca Brommel 
IOWA 
DEPARTMENT OF NATURAL RESOURCES EMERGENCY ORDER 
IN THE 
MATTER OF: 
TOM & 
RHONDA BRAKKE D/B/A PINE RIDGE HUNTING LODGE Davis County, Iowa 
EMERGENCY 
ORDER 
NO. 
2013-HP-
TO: Tom and 
Rhonda Brakke, Owners of Pine Ridge Hunting Lodge 22529 Balsam Ave. Clear Lake, 
IA 50428 
I. SUMMARY 
This 
Emergency Order is issued by the Director of the Iowa Department of Natural 
Resources ("DNR") to Tom and Rhonda Brakke D/B/A Pine Ridge Hunting Lodge ("the 
Brakkes”) for the purpose of resolving violations which occurred when the 
Brakkes removed portions of the fence surrounding the premises they manage or 
have managed as a hunting preserve, Pine Ridge Hunting Lodge, an area under 
quarantine for chronic wasting disease ("CWD"). This Emergency Order requires 
the Brakkes to stop immediately the deconstruction of the fence surrounding the 
Pine Ridge Hunting Lodge hunting preserve ("Quarantined Premises"); to restore 
immediately the portions of the fence so removed or degraded; to maintain the 
fence as an adequate quarantine around the Quarantined Premises for a period of 
five years; to close immediately and keep closed all gates to return the 
Quarantined Premises to a closed state; to authorize DNR to access the 
Quarantined Premises for a limited duration for the purposes of depopulating any 
deer that may be present; and to submit and agree to execute a plan designed to 
prevent the spread of CWD from the Quarantined Premises. 
Questions 
regarding this Emergency Order should be directed to: 
Relating to 
technical requirements: 
Relating to 
legal requirements: 
Dr. Dale 
Garner, Wildlife Bureau Chief Iowa Department of Natural Resources 502 East 
Ninth Street Des Moines, Iowa 50319 Phone: 515-281-6156 
Kelley 
Myers, Attorney for the DNR Iowa Department of Natural Resources 502 East Ninth 
Street Des Moines, Iowa 50319 Phone: 515-281-5634 
Angie 
Bruce, Wildlife Bureau Executive Officer Iowa Department of Natural Resources 
502 East Ninth Street Des Moines, Iowa 50319 Phone: 515-281-8070 
-------------------------------------------------------------------------------- 
Page 2 
IOWA 
DEPARTMENT OF NATURAL RESOURCES EMERGENCY ORDER ISSUED TO: TOM AND RHONDA BRAKKE 
D/B/A PINE RIDGE HUNTING PRESERVE 
II. 
JURISDICTION 
Pursuant to 
Iowa Code section 484C.4, which requires the Director to enforce the hunting 
preserve program and requirements; Iowa Code section 456A.23, which requires the 
DNR to protect wild animals and enforce by proper actions and proceedings the 
laws, rules and regulations relating to them; Iowa Code section 456A.25, which 
authorizes the Director to issue an order after investigation has occurred to 
secure compliance with the laws and rules DNR is entrusted to enforce; 571 Iowa 
Administrative Code ("IAC") section 115.10, requiring the Brakkes to maintain 
the quarantine for a period of at least five years; and 561 IAC section 7.18, 
which is incorporated by reference in 571 IAC chapter 7, which authorizes the 
Director to issue an emergency order requiring cessation of an activity and 
requiring an affirmative action when necessary to prevent or avoid immediate 
danger to the welfare of the state, DNR has jurisdiction to issue this Emergency 
Order. 
III. 
STATEMENT OF FACTS 
1. The wild 
deer hunting industry generates approximately $200 million annually for Iowa’s 
economy and is responsible for the creation of over 2000 jobs, annually. In 
addition, the tax revenue for the state and federal governments attributed to 
this wild deer hunting industry in Iowa is approximately $30 million combined, 
annually. In addition, wild deer are native to the State of Iowa and constitute 
a public resource held in trust by the state of Iowa. 
2. Iowa 
Code authorizes the establishment and management of hunting preserves. These 
facilities are fenced facilities wherein customers traditionally pay the 
operator to participate in a hunt on the fenced property. The deer within the 
hunting preserve are whitetail and considered preserve deer. Wild animals may 
not be caught to populate the hunting preserve (except for some remaining deer 
upon the initial erection of the fences and after diligent efforts to remove 
those wild deer); instead, the hunting preserves are populated by natural 
breeding on the preserve grounds and by introduction of farm deer provided by 
breeding facilities. 
3. 
Consistent with its statutory and administrative authorities, DNR manages 
diseases in deer, in particular CWD, because wild deer roam many hundreds of 
miles and can transmit CWD through saliva, nasal fluid, urine and excrement. The 
spread of CWD is made more virulent by virtue of the fact that CWD is not a 
virus or bacteria; it is a disease caused by transmissible spongiform 
encephalopathies (TSEs) or prions. These prions are infectious and 
self-propagating, meaning they can live without an animal host in the ground, 
and no known cure exists. The spread of this disease appears more likely where 
deer are crowded or congregated, which is why CWD testing is done at hunting 
preserves in Iowa. Other states that have experienced CWD outbreaks in the wild 
herd have not been able to stop them. The costs of CWD outbreaks are both 
financial, in lost hunting revenues for local businesses and farmers and lost 
tax revenues related to the hunting and traveling associated with hunting, as 
well as social, with the stigmatizing of one of Iowa’s cherished local 
traditions. 
4. The 
Brakkes operated the Pine Ridge Hunting Lodge ("Pine Ridge") as a hunting 
preserve, authorized by Iowa Code chapter 484C and regulated by 571 IAC chapter 
115. DNR issued the most recent license to the Brakkes to operate the hunting 
preserve at Pine Ridge from July 1, 2012 to June 30, 2013. 
-------------------------------------------------------------------------------- 
Page 3 
IOWA 
DEPARTMENT OF NATURAL RESOURCES EMERGENCY ORDER ISSUED TO: TOM AND RHONDA BRAKKE 
D/B/A PINE RIDGE HUNTING PRESERVE 
5. On July 
16, 2012, DNR received a notice from the Texas Veterinary Medical Diagnostic Lab 
("Texas Vet Lab”) that a sample from an adult male deer killed at Pine Ridge 
tested presumptively positive for CWD. (DNR has an agreement with the Texas Vet 
Lab to run these preliminary tests.) Because the Texas Vet Lab found this 
presumptive positive result, protocols required the sample to be sent to the 
National Veterinary Services Laboratory ("National Lab”) in Ames, Iowa for final 
confirmation. On July 18, 2012, the National Lab confirmed the positive CWD 
result in the deer. 
6. On July 
19, 2012, DNR notified the Brakkes of the positive test by phone. Mr. Brakke was 
out of state. 
7. On July 
23, 2012, DNR met with the Brakkes to initiate an epidemiological investigation. 
This investigation would help determine where the infected deer came from and 
make preliminary assessments about the extent of the exposure. The Brakkes 
provided information including their herd inventory and photographic evidence of 
the animals killed on the date the infected deer was killed. Also present at 
this meeting were representatives from the Iowa Department of Agriculture and 
Land Stewardship ("IDALS"), the United States Department of Agriculture ("USDA") 
and the Iowa Whitetail Deer Association, an Iowa non-profit organization. IDALS 
regulates breeding programs that sometimes populate hunting preserves. USDA 
regulates interstate transport of captive deer; its veterinarian designated as 
the Area Veterinarian in Charge would have been involved to determine if the 
diseased captive deer are or may have been moved through interstate commerce 
and/or transport. 
8. Based on 
information provided by the Brakkes, DNR concluded that captive deer killed on 
the Hunting Preserve on the same day as the infected deer were located in 
Florida, New Hampshire, Tennessee and Iowa. Between July 27, 2012 and August 6, 
2012, DNR worked with law enforcement officials from those other states to 
collect samples from the antlers of those deer for DNA testing. These tests 
would help to identify the origin of the infected deer and verify Brakke's prior 
documents that the infected deer came from the breeding facility run by the Tom 
and Rhonda Brakke in Cerro Gordo County, Iowa ("Brakke’s Breeding Facility"). 
These samples were obtained in a manner to preserve the chain of custody. 
9. On 
August 10, 2012, the Wyoming Game and Fish Wildlife Forensic and Fish Health 
Laboratory ("Wyoming Lab") provided DNR results for the seven specimens provided 
to it. (DNR has an agreement with the Wyoming Lab to conduct DNA testing.) The 
results confirmed that the infected deer originated from the Brakke's Breeding 
Facility. 
10. On 
August 13, 2012, DNR notified the Brakkes of the DNA results by telephone. DNR 
advised the Brakkes that they would need to meet with DNR to develop a plan to 
address the CWD infection at the Hunting Preserve. DNR would have also been 
communicating with IDALS consistent with the Plan. 
11. On 
September 7, 2012, DNR and the Brakkes executed an agreement ("Agreement") to 
depopulate the Hunting Preserve by January 31, 2013, and to clean and disinfect 
the Hunting Preserve. It also contained a general Compliance with Laws 
provision, which required the Brakkes to comply with all applicable federal, 
state and local laws and regulations, including without limitation the rules 
described in 571 Iowa Administrative Code section 115.10 related to the 
maintenance of a 
-------------------------------------------------------------------------------- 
Page 4 
IOWA 
DEPARTMENT OF NATURAL RESOURCES EMERGENCY ORDER ISSUED TO: TOM AND RHONDA BRAKKE 
D/B/A PINE RIDGE HUNTING PRESERVE 
quarantine 
on the Quarantined Premises and the prohibition of deer movement in or out of 
the Quarantined Premises. 
12. The 
Brakkes depopulated the Hunting Preserve, as specified in the Agreement, from 
September 10, 2012 to January 31, 2013. As part of this effort, the Brakkes, the 
staff and their customers killed 199 captive deer and nine captive elk. The DNR 
obtained 170 CWD samples. (Samples were not taken from fawns and one adult 
female who was killed in a manner that made sampling impossible.) Of these 199 
deer, two additional adult male deer tested positive for CWD. Information 
provided by the Brakkes confirmed that these two additional deer originated from 
the Brakke Breeding Facility. 
13. DNR 
installed, with the Brakke's permission, an interior electric fence on October 1 
and 2, 2012. 
14. The 
Brakkes cleaned and disinfected, under DNR supervision, the feeders and ground 
surrounding the feeders on April 5, 2013. 
15. On 
April 26, 2013, the Brakkes hand-delivered a notice to the DNR’s Chief of Law 
Enforcement Bureau, notifying the DNR that they would no longer operate a 
hunting preserve on the Quarantined Premises. The Brakkes did not reveal any 
plans to remove the fence around the Quarantined Premises or to remove the gates 
to and from the Quarantined Premises in this April 26, 2013 letter. 
16. On June 
3, 2013, DNR became aware that sections of the exterior fence surrounding the 
Quarantined Premises had been removed and that some, if not all, of the exterior 
gates to and from the Quarantined Premises were open. 
17. On June 
4, 2013, DNR received reports from the public in the area that four wild deer 
were observed inside the Quarantined Premises. 
18. On June 
5, 2013, DNR conducted a fence inspection, after gaining approval from 
surrounding landowners, and confirmed that the fenced had been cut or removed in 
at least four separate locations; that the fence had degraded and was failing to 
maintain the enclosure around the Quarantined Premises in at least one area; 
that at least three gates had been opened; and that deer tracks were visible in 
and around one of the open areas in the sand on both sides of the fence, 
evidencing movement of deer into the Quarantined Premises. 
IV. 
CONCLUSIONS OF LAW 
1. Iowa 
Code section 484C.3 authorizes the DNR to adopt rules to administer the Preserve 
Whitetail program authorized by Iowa Code chapter 484C. DNR, through the Natural 
Resource Commission, has adopted rules in 571 IAC chapter 104 and 115 to limit 
movement of captive deer, monitor diseases among captive deer and establish 
requirements for hunting preserves authorized by the Preserve Whitetail program. 
-------------------------------------------------------------------------------- 
Page 5 
IOWA 
DEPARTMENT OF NATURAL RESOURCES EMERGENCY ORDER ISSUED TO: TOM AND RHONDA BRAKKE 
D/B/A PINE RIDGE HUNTING PRESERVE 
2. Iowa 
Code section 484C.4 authorizes the DNR to develop, administer and enforce 
hunting preserve programs consistent with the requirements both in Iowa Code 
chapter 484C and rules promulgated pursuant thereto. 
3. Iowa 
Code section 484C.6(1) requires fences installed in and around hunting preserves 
to comply with rules adopted by the DNR; this requirement would include the 
fencing requirements found in 571 IAC section 115.10 and discussed in paragraph 
6 of this section below. The Agreement, through the Compliance with Laws 
provision, requires the maintenance of a quarantine on the Quarantined Premises 
and the prohibition of deer movement in or out of the Quarantined Premises; such 
requirement would survive termination of the Agreement by virtue of being law. 
4. Iowa 
Code section 484C.12(1) requires that any whitetail deer confined in hunting 
preserves are free of diseases and authorizes DNR to establish a quarantine for 
the infected whitetail population. The facts stated above demonstrate that CWD 
was found within the Brakkes whitetail population at the Quarantined Premises 
and that Brakkes were knowledgeable of such determination. 
5. Iowa 
Code section 484C.12(2) requires the landowner and an epidemiologist appointed 
by the DNR, to develop a plan in the event a reportable disease, such as CWD, is 
found. The plan must include the eradication of the reportable disease among the 
preserve whitetail population as well as a plan both to reduce and eliminate the 
reportable disease and to prevent the spread of disease to other animals. The 
plan must comply with the DNR's rules, must be approved by the DNR and must be 
incorporated into an agreement between the landowner, or the Iandowner’s 
veterinarian, and epidemiologist appointed by the DNR. The facts stated above 
demonstrate that the Brakkes worked with the DNR to depopulate the Quarantined 
Area but that the Brakkes did not develop a future operational plan to address 
how they would continue to prevent the spread of disease to other animals. 
6. 571 IAC 
section 115.10 requires a five-year quarantine be placed on a preserve and any 
remaining animals on the preserve when CWD is found in any animals on a 
preserve. (Currently CWD is only found in the animal family cervidae, which 
include deer and elk.) The rules prohibit animal movement in or out of the 
preserve during the quarantine period. The facts stated above demonstrate that 
the Brakkes have violated this rule requirement in their removal of the fence 
and opening ofthe gates. 
7. Iowa 
Code section 17A.18A authorizes the Director of the DNR to take action necessary 
to prevent or avoid immediate danger to the public welfare. The Brakke’s failure 
to maintain the quarantine of the Quarantined Premises will allow for the spread 
of CWD beyond a captive herd. Once wild deer are exposed to CWD that exists on 
the Quarantined Area, DNR will not have a mechanism to limit the exposure to the 
remainder of the wild deer population. Iowa's wild deer herd serves a 
multi-million dollar industry in the state of Iowa and provides over $15 million 
in tax revenue to the state. The spread of CWD into the wild population would 
cripple the whitetail hunting industry in Iowa. 
8. 561 IAC 
section 7.18 (which is incorporated by reference in 571 IAC chapter 7) 
authorizes the Director of the DNR to issue this Emergency Order to the extent 
necessary to prevent 
-------------------------------------------------------------------------------- 
Page 6 
IOWA 
DEPARTMENT OF NATURAL RESOURCES EMERGENCY ORDER ISSUED TO: TOM AND RHONDA BRAKKE 
D/B/A PINE RIDGE HUNTING PRESERVE 
or avoid 
immediate danger to the welfare of the citizens of Iowa. This authority 
authorizes the Director of the DNR to require a person or persons to immediately 
cease an offending activity or take an affirmative action to avoid or mitigate 
the offending action. This emergency action is necessary for the reasons stated 
in paragraph 7 of this section. 
V. ORDER 
THEREFORE, 
DNR orders the Brakkes to do the following immediately: 
1. The 
Brakkes shall immediately discontinue the deconstruction of the fence 
surrounding the Quarantined Premises. 
2. The 
Brakkes shall completely restore the portions of the fence surrounding the 
Quarantined Premises that have been removed or degraded by no later than June 7, 
2013. 
3. The 
Brakkes shall maintain the fence surrounding the Quarantined Premises, as an 
adequate quarantine until December 28, 2017, which is a period of five years 
from date of the last positive CWD diagnosis on the Quarantined Premises, which 
was December 28, 2012. 
4. The 
Brakkes shall close by June 7, 2013, and keep closed, all gates to and from the 
Quarantined Premises to return the Quarantined Premises to a closed state. 
5. The 
Brakkes shall authorize DNR officials to access the Quarantined Premises for a 
period of four weeks from date the Brakkes satisfy paragraphs 1 and 2 of this 
section for the purposes of depopulating any deer that may be on the Quarantined 
Premises. DNR shall recover and test those deer, if any, for CWD and report the 
findings to the Brakkes. 
6. The 
Brakkes shall submit an operational plan, consistent with Iowa Code section 
484C.12(2) that demonstrates how they shall comply with the quarantine 
requirements imposed by 571 IAC section 115.10. It shall be designed to prevent 
the spread of CWD to other animals. Such plan shall be reviewed by the DNR and 
shall be memorialized in an agreement among the Brakkes or the Brakkes’ 
veterinarian, the DNR and the State of Iowa's epidemiologist. 
VI. HEARING 
Pursuant to 
Iowa Code section 17A.18A(5), this Emergency Order is being transmitted to the 
Department of Inspections and Appeals so that the order may be set for immediate 
hearing before an Administrative Law Judge. You will be promptly notified of the 
hearing time and place. Consistent with 561 IAC section 7.18(5) (incorporated by 
reference into 571 IAC chapter 7), you may seek a stay of this Emergency Order 
consistent with the procedures identified therein. 
VII. 
NONCOMPLIANCE 
Failure to 
comply with this Emergency Order may result in referral of this matter to the 
Attorney General to obtain injunctive relief, any civil penalties authorized by 
Iowa Code section 484C.13 and any civil damages attributed to the spread of CWD 
from the Quarantined Premises to 
-------------------------------------------------------------------------------- 
Page 7 
IOWA 
DEPARTMENT OF NATURAL RESOURCES EMERGENCY ORDER ISSUED TO: TOM AND RHONDA BRAKKE 
D/B/A PINE RIDGE HUNTING PRESERVE 
the wild 
and other captive herds in the state or to the Davis County Attorney's Office 
for criminal prosecution consistent with Iowa Code section 484C.13. 
CHUCK GIPP, 
DIRECTOR Iowa Department of Natural Resources
Dated this 
6th day of June, 2013
CC: Kelley 
Myers 
Iowa Judge 
Rules against IDALS Motion to Consolidate Brakke Cases
July 23, 
2013 Iowa Judge Rules against IDALS Motion to Consolidate Brakke Cases Iowa 
Assistant Attorney General to Inquire Constitutionality of CWD Standards 
Rationale Today, July 23, 2013, the Iowa Administrative Legal Judge ruled 
against a motion request by the Iowa Department of Land Stewardship (IDALS) to 
merge the two contested cases (Ag and IDNR) filed by the Brakke family into one. 
The motion would also delay hearing the merits for both cases until November. 
Tom and Rhonda Brakke filed suit against IDALS last fall to seek indemnity for 
their animals on their breeder farm. The Brakke’s filed a second suit against 
the Iowa Department of Natural Resources (IDNR) in July 2013, after the IDNR 
issued an emergency order quarantining their 330 acres until December 28, 2017. 
The Brakke’s believe the two lawsuits are separate jurisdictions and 
constitutional takings even though they are both related to the exposure of 
Chronic Wasting Disease. In a statement issued by the Iowa Assistant Attorney 
General, "Both cases will involve a constitutional takings analysis, and that 
analysis will involve some level of discussion of the cience and rationale 
behind IDALS' and DNR's Chronic Wasting Disease (CWD) quarantines policies and 
the USDA's Program Standards for CWD exposed herds or property." The American 
Cervid Alliance will distribute updates regarding the Iowa cases as they become 
available.
Greetings 
everyone, a few comments here, my opinion. 
open gates, 
fences cut, seems like intent to infect Iowa’s wild deer with CWD to me. who, 
and why, would anyone do such a thing? seems crazy to me. I don’t understand why 
the Iowa state tax payers are responsible for any part of the cleanup of the 
mess the Brakkes or any other deer game farms are responsible for. the tax 
payers in Iowa should be up in arms over that. that’s why I think the laws for 
these shooting pens should have to be made mandatory to have a 10 million dollar 
insurance policy or more, for any cleanup cost for such incidents TO that state, 
and that the state should not have to foot the bill at all, if they are going to 
operate at all. the state should not have to negotiate anything, in order to 
maintain and preserve the health and integrity of any states wild cervids, once 
negligence is proven. just my opinion. ...kind regards, terry
| Today, 07:23 AM | #21 | |
| 
Senior 
Member 
Join Date: Sep 
2012 
Location: Clear Lake, 
Iowa 
Posts: 136 
 | 
Please see the bottom of the link below. You will soon 
see testimony from our court case, Dr. Miller. The state of Iowa testified that 
CWD is bad enough to take our business, take our land for five years, and force 
us to destroy our animals, pay tor test and dispose of them at our own expense 
or continue feeding them forever essentially. BUT it is not highly infectious or 
highly contagious, nor a risk to public wealth health or welfare so they do not 
feel they need to compensate us .....  NAEBA, EWA, and the ACA support scrapping this document. Again, there is a NADeFA board meeting Thursday night. Why is NADeFA still supporting this document? Is the $3 million contingent on the Standards document implementation?????? Oh and yes, the ACA has raised money for us but cannot give it to us because their status is tied up with the IRS. I suppose we will have pay our attorneys check into that as well. Not only do we have HSUS writing our regulations, Ms. Klein also worked for Fish and Game for six years prior to taking her position with the USDA. 
Quote: 
 | 
| 07-25-2013, 05:37 AM | #12 | 
| 
Senior 
Member 
Join Date: Sep 
2012 
Location: Clear Lake, 
Iowa 
Posts: 129 
 | 
Because I find it increasingly difficult to sleep at 
night and still find it important to educate the membership and it appears a 
number of you do not sign in to the members only area: Dear Fellow Producer: To provide you with an update, Tom and I filed suit with the Iowa Department of Agriculture and Land Stewardship (IDALS) last fall for compensation for the animals at our breed facility. Following complete depopulation of our animals at our hunting preserve at our expense, IDALS dropped the quarantine, we completed clean-up and canceled our hunting preserve license. Upon opening the gates, the Iowa Department of Natural Resources served an Emergency Order requiring that we close the gates and reinstall electric fence around the 330 acre property, along with quarantine orders through December 28, 2017. IDALS requested that their portion of the suit be consolidated and continued with the IDNR suit in November. In their motion to consolidate and continue IDALS report: "Both cases will involve a constitutional takings analysis, and that analysis will involve some level of discussion of the science and rational behind IDALS' and DNR's Chronic Wasting Disease (CWD) quarantine policies and the USDA's Program Standards for CWD exposed herds or property." As of today, July 23, 2013, the judge ruled to deny IDALS's motion to continue and consolidate, therefore, full merits will be herd for the quarantine of the breed facility animals in August. The IDNR merits for the 330 acre hunting preserve property is scheduled for November. Again, our hunting preserve property is valued at approximately $1.5 million, and now quarantined until December 28, 2017. Our breed herd of 500+ animals appraised at approximately 1.5 million and costing roughly $3,000 per week to feed and our breed facility land valued at a minimum of $245,000, also quarantined for five years minimum. We have spent more than $160,000 in attorney fees fighting for our property rights and due compensation. Following our 10 year certification for CWD monitoring we dropped out of the CWD program, neither the breed facility nor the hunting preserve were part of the monitoring program nor have we signed any agreement with the State of Iowa or USDA for quarantine and yet we are effected by CWD exposure through the CWD Rule and Standards. Unfortunately, there are still a number of producers whom simply do not understand the imploding impacts from the CWD Standards for every producer. The Standards are not only impacting our case here in Iowa, they will impact each of you no matter which state you or your herd and property reside. As in our case, common ownership will override any and all justifications for quarantine, regardless if you do or do not participate in the "voluntary" CWD program. While I understand, we may get $3 million put back into the USDA CWD program, of that $3 million a minimum of $1.1 million will be allocated for salaries, and the remaining 1.9 million toward research and compensation. How far is this really going to get Tom and I or the next producer exposed to CWD? A question we often hear, "What can I do?" Our response: Become a united industry in the movement against government overreach by the policy makers. We ask that you think about what you have invested in your operation(s) and then think about what you are willing to risk if or when your herd or property is exposed to CWD. If these Standards are something you can live with should you become exposed to CWD, then you have made a conscious choice to support the Standards document. However, if you chose to become united for science-based disease management, property rights and due compensation; then we ask that you get involved and work to make changes and stop putting we producers out of business and stop taking our property without due compensation. A "voluntary" program made "involuntary" through our ability for movement or commerce without compensation, is unjust and needs to be changed. The Standards Document is due for public comment any day. Please take the first step, keep your antenna up, and submit your comments. God Bless and thank you for your continued support! Best Regards, Tom and Rhonda Brakke ----------- As reported by USDA it appears I may have underestimated the program salaries and "activities" in my above letter: In FY2012 approximately $1.925 million of ECSR funding was allocated for CWD program activities to provide Federal oversight of the national CWD herd certification program (HCP).------------- I removed the producers name and buck from this horrible aritlce: July 11, 2013 _______ (Buck), the _________ Ranch's prized buck, is a semen-producing cash cow. In magazine advertisements in which ____ (Buck) is backlit in messianic grandeur, his value can be determined in other ways. ______ (Producer) sells half-cubic-centimeter straws of the animal's cryogenically frozen semen (or about a tenth of a teaspoon) for $5,000 a pop. And breeders will pony up just for a shot at a fawn boasting the great _____ (Buck) as sire. Bear in mind, a buck in his prime with an electroejaculator inserted in his rectum can produce 60 straws at a time. Though ______ (Buck) never leaves the confines of _______ Ranch, FedEx spreads his cryogenically frozen seed far and wide. From Standards Vs. 22 Document: (2.6) Additions of Animals or Genetic Material (Germplasm) to a Herd: Effects on Status A herd may add animals from herds with the same or a greater status in the national CWD HCP with no negative impact on the status of the receiving herd. If animals are acquired from a herd with a lesser status, the receiving herd reverts to the status of that source herd. If a herd participating in the program acquires animals from a nonparticipating herd, the receiving herd reverts to First Year status with a new status date listed as the date of acquisition of the animal. The enrollment date in the national CWD HCP would remain unchanged but the herd status level would be modified (and modification date recorded). If a herd acquires animals from herds with a lower or nonparticipating status, the owner must notify a Federal or State official within 5 business days of such acquisition. At this time there is no scientific evidence that germplasm (embryos or semen) may transmit CWD. . If scientific evidence of the role of embryos or semen in the transmission of CWD should become available, this guidance will be changed. We tried to remove this section from the Standards document, but failed. ---------------- A report from USAHA which I attended in N.C. last fall and I helped to draft the below amendment. This is all Tom and I were requesting for our investments and property, but we were served Part B of the Standards because it was reported by some industry leaders that the Standards Document really wasn't that bad. RESOLUTION NUMBER: 20– APPROVED SOURCE: Committee on Captive Wildlife and Alternative Livestock SUBJECT MATTER: Chronic Wasting Disease Control BACKGROUND INFORMATION: It has been stated by the United States Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services that (1) the goal of the Chronic Wasting Disease (CWD) program in the United States has now changed from eradication to controlling its spread, (2) there is no longer federal funding available to pay for CWD testing or to pay indemnity for CWD infected or exposed animals, and (3) depopulation of infected herds will no longer be required or expected. With this major change in objectives, it is critical that we change the way we implement the CWD program in the United States. We now need a program that minimizes the risk of spreading CWD in farmed and wild cervidae without putting farmed cervidae producers out of business if their herds become CWD infected or exposed. We need a CWD control program that includes plans for how to (1) handle infected or exposed herds, (2) clean up infected herds without depopulation, and (3) provide outlets so producers can continue to sell velvet antler and live animals to slaughter or specified terminal facilities. We had a 7 hour deposition yesterday in Des Moines and returning again today and tomorrow. Even though the Standards document has not been approved or put to public comment period or even implemented, it was a discussed in great deal yesterday. --------- We have been put out of business, smeared in the newspapers across the state and the country to the unsuspecting public leading them to believe that our positive case of CWD is going to destroy the entire deer population in Iowa, infect cattle, infect the surrounding hay and harvest ground outside of our Davis County property and people, including a USAHA release with an Iowa Representative call us irresponsible, there is no way to undo the damage that we have been served. Last edited by Rhonda Brakke; 07-25-2013 at 05:48 AM. | 
| 07-25-2013, 06:30 AM | #13 | 
| 
Senior 
Member 
Join Date: Sep 
2012 
Location: Clear Lake, 
Iowa 
Posts: 129 
 | 
I 
should note that the Standards Vs. 22 document does include movement of positive 
herds to a "designated" hunting facility, BUT they have included such 
restrictions that it will make it very difficult, if not impossible, to find 
such a facility within your own state, not to mention, one that is willing to 
take your "exposed" animals. I have attended several meetings in which state 
vets have said that they would not allow exposed animals to move in their state 
or across state lines. I would recommend that you ask your own state vet how 
they would feel about moving "exposed" animals either in your state or across 
state lines. Again from the Standards Vs. 22 Document: A herd plan may also contain additional requirements to prevent or control the possible spread of CWD, depending on the particular condition of the herd and its premises, including but not limited to: 1. Depopulation of the herd if funds for indemnity are available. Depopulation also may be accomplished by moving animals from CWD positive, suspect, and exposed herds by permit and under seal to a slaughter facility or to an appropriate hunt facility either within the state or to an appropriate hunt facility in CWD endemic areas at the discretion of the state officials.2. Specifying the time for which a premises must not contain cervids after CWD-positive diagnosis. 3. Removal of exposed or suspect animals from the premises if funds for indemnity are available or at the discretion of the state officials. 4. Fencing requirements. 5. Selective culling of animals. (At who's discretion?) 6. Restrictions on use and movement of possibly contaminated livestock equipment. 7. Cleaning and disinfection requirements, or other requirements. If a positive or exposed herd is depopulated, the written herd plan will consist only of premises cleaning and disinfection and restocking requirements. | 
| 08-19-2013, 07:44 PM | #1 | 
| 
Senior 
Member 
Join Date: Sep 
2012 
Location: Clear Lake, 
Iowa 
Posts: 136 
 | 
As 
reported in court today, Iowa has 77 Scrapies positive flocks since 2001. Have 
you read the requirements for Scrapies when animals become exposed. If you 
haven't I suggest you do. Have you read the quarantine requirements for animals 
and land, or lack thereof? For the life of my grandchildren, I cannot understand 
what we Re doing to our own industry. NADeFA is holding a board meeting Thursday 
night.... Today was the last day of court today for our breed facility. Full report tomorrow. Thank you all for your continued support. Was a very informative day, once again. | 
Wednesday, July 31, 2013 
Iowa Brakke Family Farmed CWD 
livestock update July 3, 2013 
> These "nonclassical" cases are 
cases that have been proven to spontaneously 
occur.
please note, the above statement is 
absolutely NOT TRUE, NEVER PROVEN! there has never been a documented case of TSE 
prion disease in the wild proven to be a spontaneous case, never has happened. 
this is total fabrication of science, or what I call store bought junk science 
bought and paid for by usda inc and the oie et al. ...
tss
What irks many scientists is the USDA’s April 25 statement that the rare 
disease is “not generally associated with an animal consuming infected 
feed.”
The USDA’s conclusion is a “gross oversimplification,” said Dr. Paul Brown, 
one of the world’s experts on this type of disease who retired recently from the 
National Institutes of Health. "(The agency) has no foundation on which to base 
that statement.”
“We can’t say it’s not feed related,” agreed Dr. Linda Detwiler, an 
official with the USDA during the Clinton Administration now at Mississippi 
State.
In the May 1 email to me, USDA’s Cole backed off a bit. “No one knows the 
origins of atypical cases of BSE,” she said
The argument about feed is critical because if feed is the cause, not a 
spontaneous mutation, the California cow could be part of a larger outbreak. 
Thursday, August 15, 2013 
Stability properties of PrPSc from cattle with experimental transmissible 
spongiform encephalopathies: use of a rapid whole homogenate, protease-free 
assay 
====================================
XXXXXX--IMPORTANT TSE HISTORY--XXXXXX
====================================
greetings, my feelings about shooting pens are my feelings, they are not 
law. the science I try to bring to the public domain, it speaks for itself, and 
has for a great many years, via a bunch of wonderful dedicated scientist around 
the world. rhonda brings up a very good point above, on the difference in 
scrapie and CWD regulations (for game farmers regulations). let me tell you 
this. the usda inc is doing it’s best to eliminate all TSE prion regulations 
through the OIE. if you shooting pen owners could get hooked up with the OIE as 
the USDA inc is, it would be all clear sailing for you then, because that is the 
goal of the OIE as well. 
I 
thought this might be important for you game farmers, you might find it 
interesting, because I do agree that you are being dealt with differently in 
regards to cwd regulations and scrapie, but how I see the problem and risk 
factors from the TSE prion disease due to this disregard of science, and how the 
shooting pen owners would look at it, is different. the shooting pens are, if I 
understand it correctly, are upset because they don’t have the same regulations 
as scrapie, and ramifications financially there from, and the shooting pens want 
their regulations weakened to the scrapie regulations level. ...give it time, 
you’ll be good to go, because the usda inc. et al threw sound science out the 
window a long time ago. ...cheers!
====================================
XXXXXX--IMPORTANT TSE HISTORY--XXXXXX
====================================
6. 
WHAT happened to the test results and MOUSE BIO-ASSAYS of those 
> imported sheep from Belgium that were confiscated and slaughtered 
from the 
> Faillace's, what sort of TSE did these animals have ? Imported 
> Belgium/Netherlands Sheep Test Results Background Factsheet 
Veterinary 
> Services April 2002 APHIS ...snip...
>
> 7. WHY is it that the Farm of the Mad Sheep of Mad River Valley 
were 
> quarantined for 5 years, but none of these farms from Texas and 
Alabama 
> with Atypical TSE in the Bovine, they have not been quarantined for 
5 
> years, why not, with the real risk of BSE to sheep, whom is to say 
this 
> was not BSE ? (see later results via FOIA below...TSS)...snip
Tuesday, November 13, 2007
DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E. (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES [Docket No. 00-072-1]
To: Garfield.O.Daley@aphis.usda.gov
CC: phyllis.Fong@usda.gov; bse-L@aegee.org;
Re: FOIA APPEAL 07-566 DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E. (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES [Docket No. 00-072-1]
CC: phyllis.Fong@usda.gov; bse-L@aegee.org;
Re: FOIA APPEAL 07-566 DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E. (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES [Docket No. 00-072-1]
EXACTLY WHAT are these people capable of doing ???
JUST HOW FAR will they go ???
Mad Sheep The True Story Behind the USDA‚ War on a Family Farm Linda Faillace
The page-turning account of a government cover-up, corporate greed, and a courageous family‚ fight to save their farm.
FOIA OF DECLARATION OF 
EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E. (PRION DISEASE) OF FOREIGN 
ORIGIN IN THE UNITED STATES [Docket No. 00-072-1]
Thursday, April 24, 
2008
RE-FOIA OF DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E. OF FOREIGN ORIGIN IN THE UNITED STATES [Docket No. 00-072-1]
RE-FOIA OF DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E. OF FOREIGN ORIGIN IN THE UNITED STATES [Docket No. 00-072-1]
Saturday, February 27, 
2010
IN SHORT ;
August 15, 2000
OIG case # NY-3399-56 REDACTED, VT
''Enclosed is OIG's notification that they have scheduled an investigation of the following individual. REDACTED is alleged to have provided possibly inaccurate test results involving diseased sheep. However, because the results were determined to be inconclusive, no actual violation was actually committed.''
August 15, 2000
OIG case # NY-3399-56 REDACTED, VT
''Enclosed is OIG's notification that they have scheduled an investigation of the following individual. REDACTED is alleged to have provided possibly inaccurate test results involving diseased sheep. However, because the results were determined to be inconclusive, no actual violation was actually committed.''
snip...
PLEASE SEE FULL TEXT HERE ;
> >> Imported
> >> Belgium/Netherlands
> >> Sheep Test Results
> >> Background
> >> Factsheet
> >> Veterinary Services April 2002
> >> APHIS
> >
> >
> >
> > snip...
> >
> >> Additional tests will be conducted to determine
> >> exactly what TSE the animals have BSE or scrapie.
> >> These tests involve the use of bioassays that consist
> >> of injecting mice with tissue from the infected animals
> >> and waiting for them to develop disease. This testing
> >> may take at least 2 to 3 years to complete.
> >
> >
> >
> > http://www.aphis.usda.gov/lpa/pubs/fsheet_faq_notice/fs_ahvtsheeptr.pdf
>
>
> >
> > DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E.
> > (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES
> >
> >
>
http://frwebgate.access.gpo.gov/cgi-bin/getdoc.cgi?dbname=2000_register&docid=fr20jy00-31
>
>
>
> >
> >
> > DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E
> > (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES [2]
> >
> >
>
http://frwebgate.access.gpo.gov/cgi-bin/getdoc.cgi?dbname=2000_register&docid=fr20jy00-32
>
>
>
> >
> >
> > or if those old urls dont work, go here;
> >
> > DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E
> > (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES
> > - Terry S.
> > Singeltary Sr. 7/20/00 (0)
> >
>
> > [Federal Register: July 20, 2000 (Volume 65, Number 140)] [Notices]
> > [Page 45018] >From the Federal Register Online via GPO Access
> > [wais.access.gpo.gov] [DOCID:fr20jy00-32]
> >
> > -----------------------------------------------------------------------
> >
> > DEPARTMENT OF AGRICULTURE
> >
> > Office of the Secretary
> >
> > [Docket No. 00-072-1]
> >
> > Declaration of Extraordinary Emergency Because of an Atypical
> > Transmissible Spongiform Encephalopathy (Prion Disease) of Foreign
Origin
> >
> > A transmissible spongiform encephalopathy (TSE) (prion disease) of
> > foreign origin has been detected in the United States. It is different
> > from TSE's previously diagnosed in the United States. The TSE was
> > detected in the progeny of imported sheep. The imported sheep and
> > their progeny are under quarantine in Vermont. Transmissible
> > spongiform encephalopathies are degenerative fatal diseases that can
> > affect livestock. TSE's are caused by similar, as yet uncharacterized,
> > agents that usually produce spongiform changes in the brain.
> > Post-mortem analysis has indicated positive results for an atypical
> > TSE of foreign origin in four sheep in Vermont. Because of the
> > potentially serious consequences of allowing the disease to spread to
> > other livestock in the United States, it is necessary to seize and
> > dispose of those flocks of sheep in Vermont that are affected with or
> > exposed to the disease, and their germ plasm. The existence of the
> > atypical TSE of foreign origin represents a threat to U.S. livestock.
> > It constitutes a real danger to the national economy and a potential
> > serious burden on interstate and foreign commerce. The Department has
> > reviewed the measures being taken by Vermont to quarantine and
> > regulate the flocks in question and has consulted with appropriate
> > officials in the State of Vermont. Based on such review and
> > consultation, the Department has determined that Vermont does not have
> > the funds to compensate flock owners for the seizure and disposal of
> > flocks affected with or exposed to the disease, and their germ plasm.
> > Without such funds, it will be unlikely to achieve expeditious
> > disposal of the flocks and germ plasm. Therefore, the Department has
> > determined that an extraordinary emergency exists because of the
> > existence of the atypical TSE in Vermont. This declaration of
> > extraordinary emergency authorizes the Secretary to seize, quarantine,
> > and dispose of, in such manner as he deems necessary, any animals that
> > he finds are affected with or exposed to the disease in question, and
> > their germ plasm, and otherwise to carry out the provisions and
> > purposes of the Act of July 2, 1962 (21 U.S.C. 134-134h). The State of
> > Vermont has been informed of these facts.
> >
> > Dated: This declaration of extraordinary emergency shall become
> > effective July 14, 2000. Dan Glickman, Secretary of Agriculture. [FR
> > Doc. 00-18367 Filed 7-19-00; 8:45 am] BILLING CODE 3410-34-P
> >
> >
>http://frwebgate.access.gpo.gov/cgi-bin/getdoc.cgi?dbname=2000_register&docid=fr20jy00-32
> > DEPARTMENT OF AGRICULTURE
> >
> > Office of the Secretary
> >
> > [Docket No. 00-072-1]
> >
> > Declaration of Extraordinary Emergency Because of an Atypical
> > Transmissible Spongiform Encephalopathy (Prion Disease) of Foreign
Origin
> >
> > A transmissible spongiform encephalopathy (TSE) (prion disease) of
> > foreign origin has been detected in the United States. It is different
> > from TSE's previously diagnosed in the United States. The TSE was
> > detected in the progeny of imported sheep. The imported sheep and
> > their progeny are under quarantine in Vermont. Transmissible
> > spongiform encephalopathies are degenerative fatal diseases that can
> > affect livestock. TSE's are caused by similar, as yet uncharacterized,
> > agents that usually produce spongiform changes in the brain.
> > Post-mortem analysis has indicated positive results for an atypical
> > TSE of foreign origin in four sheep in Vermont. Because of the
> > potentially serious consequences of allowing the disease to spread to
> > other livestock in the United States, it is necessary to seize and
> > dispose of those flocks of sheep in Vermont that are affected with or
> > exposed to the disease, and their germ plasm. The existence of the
> > atypical TSE of foreign origin represents a threat to U.S. livestock.
> > It constitutes a real danger to the national economy and a potential
> > serious burden on interstate and foreign commerce. The Department has
> > reviewed the measures being taken by Vermont to quarantine and
> > regulate the flocks in question and has consulted with appropriate
> > officials in the State of Vermont. Based on such review and
> > consultation, the Department has determined that Vermont does not have
> > the funds to compensate flock owners for the seizure and disposal of
> > flocks affected with or exposed to the disease, and their germ plasm.
> > Without such funds, it will be unlikely to achieve expeditious
> > disposal of the flocks and germ plasm. Therefore, the Department has
> > determined that an extraordinary emergency exists because of the
> > existence of the atypical TSE in Vermont. This declaration of
> > extraordinary emergency authorizes the Secretary to seize, quarantine,
> > and dispose of, in such manner as he deems necessary, any animals that
> > he finds are affected with or exposed to the disease in question, and
> > their germ plasm, and otherwise to carry out the provisions and
> > purposes of the Act of July 2, 1962 (21 U.S.C. 134-134h). The State of
> > Vermont has been informed of these facts.
> >
> > Dated: This declaration of extraordinary emergency shall become
> > effective July 14, 2000. Dan Glickman, Secretary of Agriculture. [FR
> > Doc. 00-18367 Filed 7-19-00; 8:45 am] BILLING CODE 3410-34-P
> >
> >
>http://frwebgate.access.gpo.gov/cgi-bin/getdoc.cgi?dbname=2000_register&docid=fr20jy00-32
THE 
REST IS HISTORY, around 2005, what they were trying to stop at the Faillaces 
front door, was then finally documented anyway, and since then, the Nor-98 
‘foreign animal disease’, has spread from coast to coast in North America.
*** 
The discovery of previously unrecognized prion diseases in both humans and 
animals (i.e., Nor98 in small ruminants) demonstrates that the range of prion 
diseases might be wider than expected and raises crucial questions about the 
epidemiology and strain properties of these new forms. We are investigating this 
latter issue by molecular and biological comparison of VPSPr, GSS and Nor98. 
Increased Atypical Scrapie Detections
Press reports indicate that increased surveillance is catching what 
otherwise would have been unreported findings of atypical scrapie in sheep. In 
2009, five new cases have been reported in Quebec, Ontario, Alberta, and 
Saskatchewan. With the exception of Quebec, all cases have been diagnosed as 
being the atypical form found in older animals. Canada encourages producers to 
join its voluntary surveillance program in order to gain scrapie-free status. 
The World Animal Health will not classify Canada as scrapie-free until no new 
cases are reported for seven years. The Canadian Sheep Federation is calling on 
the government to fund a wider surveillance program in order to establish the 
level of prevalence prior to setting an eradication date. Besides long-term 
testing, industry is calling for a compensation program for farmers who report 
unusual deaths in their flocks.
Thursday, March 29, 2012
atypical Nor-98 Scrapie has spread from coast to coast in the USA 2012 
NIAA Annual Conference April 11-14, 2011San Antonio, Texas
Monday, April 25, 2011
Experimental Oral Transmission of Atypical Scrapie to Sheep
Volume 17, Number 5-May 2011 However, work with transgenic mice has 
demonstrated the potential susceptibility of pigs, with the disturbing finding 
that the biochemical properties of the resulting PrPSc have changed on 
transmission (40). 
*** The discovery of previously 
unrecognized prion diseases in both humans and animals (i.e., Nor98 in small 
ruminants) demonstrates that the range of prion diseases might be wider than 
expected and raises crucial questions about the epidemiology and strain 
properties of these new forms. We are investigating this latter issue by 
molecular and biological comparison of VPSPr, GSS and Nor98. 
Friday, July 26, 
2013
Voluntary Scrapie 
Program USA UPDATE July 26, 2013 increase in FY 2013 is not 
statistically meaningful due to the sample size 
Thursday, November 18, 2010
Increased susceptibility of human-PrP transgenic mice to bovine 
spongiform encephalopathy following passage in sheep
Monday, November 30, 2009 
USDA AND OIE COLLABORATE TO EXCLUDE 
ATYPICAL SCRAPIE NOR-98 ANIMAL HEALTH CODE 
Thursday, December 20, 2012 
OIE GROUP RECOMMENDS THAT SCRAPE PRION 
DISEASE BE DELISTED AND SAME OLD BSe WITH BOVINE MAD COW DISEASE 
*** The discovery of previously 
unrecognized prion diseases in both humans and animals (i.e., Nor98 in small 
ruminants) demonstrates that the range of prion diseases might be wider than 
expected and raises crucial questions about the epidemiology and strain 
properties of these new forms. We are investigating this latter issue by 
molecular and biological comparison of VPSPr, GSS and Nor98. 
 Thursday, May 
30, 2013 
World Organization for Animal Health (OIE) has upgraded the United States' risk classification for mad cow disease to "negligible" from "controlled", and risk further exposing the globe to the TSE prion mad cow type disease
U.S. gets top mad-cow rating from international group and risk further exposing the globe to the TSE prion mad cow type disease
World Organization for Animal Health (OIE) has upgraded the United States' risk classification for mad cow disease to "negligible" from "controlled", and risk further exposing the globe to the TSE prion mad cow type disease
U.S. gets top mad-cow rating from international group and risk further exposing the globe to the TSE prion mad cow type disease
Tuesday, August 13, 2013 
Government argues against bid for court to block COOL 
Saturday, July 6, 2013
Small Ruminant Nor98 Prions Share Biochemical Features with Human Gerstmann-Sträussler-Scheinker Disease and Variably Protease-Sensitive Prionopathy
Research Article
http://nor-98.blogspot.com/2013/07/small-ruminant-nor98-prions-share.html
pens, pens, PENS ??? 
*** 
Spraker suggested an interesting explanation for the occurrence of CWD. The deer 
pens at the Foot Hills Campus were built some 30-40 years ago by a Dr. Bob 
Davis. At or abut that time, allegedly, some scrapie work was conducted at this 
site. When deer were introduced to the pens they occupied ground that had 
previously been occupied by sheep. 
now, decades later ; 
2012 
PO-039: A comparison of scrapie and chronic wasting disease in 
white-tailed deer 
Justin Greenlee, Jodi Smith, Eric Nicholson US Dept. Agriculture; 
Agricultural Research Service, National Animal Disease Center; Ames, IA USA 
snip...
The 
results of this study suggest that there are many similarities in the 
manifestation of CWD and scrapie in WTD after IC inoculation including early and 
widespread presence of PrPSc in lymphoid tissues, clinical signs of depression 
and weight loss progressing to wasting, and an incubation time of 21-23 months. 
Moreover, western blots (WB) done on brain material from the obex region have a 
molecular profile similar to CWD and distinct from tissues of the cerebrum or 
the scrapie inoculum. However, results of microscopic and IHC examination 
indicate that there are differences between the lesions expected in CWD and 
those that occur in deer with scrapie: amyloid plaques were not noted in any 
sections of brain examined from these deer and the pattern of immunoreactivity 
by IHC was diffuse rather than plaque-like. After a natural route of exposure, 
100% of WTD were susceptible to scrapie. Deer developed clinical signs of 
wasting and mental depression and were necropsied from 28 to 33 months PI. 
Tissues from these deer were positive for PrPSc by IHC and WB. Similar to IC 
inoculated deer, samples from these deer exhibited two different molecular 
profiles: samples from obex resembled CWD whereas those from cerebrum were 
similar to the original scrapie inoculum. On further examination by WB using a 
panel of antibodies, the tissues from deer with scrapie exhibit properties 
differing from tissues either from sheep with scrapie or WTD with CWD. Samples 
from WTD with CWD or sheep with scrapie are strongly immunoreactive when probed 
with mAb P4, however, samples from WTD with scrapie are only weakly 
immunoreactive. In contrast, when probed with mAb’s 6H4 or SAF 84, samples from 
sheep with scrapie and WTD with CWD are weakly immunoreactive and samples from 
WTD with scrapie are strongly positive. This work demonstrates that WTD are 
highly susceptible to sheep scrapie, but on first passage, scrapie in WTD is 
differentiable from CWD. 
2011 
*** 
After a natural route of exposure, 100% of white-tailed deer were susceptible to 
scrapie. 
Scrapie in Deer: Comparisons and Contrasts to Chronic Wasting Disease 
(CWD) 
Justin J. Greenlee of the Virus and Prion Diseases Research Unit, 
National Animal Disease Center, ARS, USDA, Ames, IA 
snip...
This highlights the facts that 1) prior to the onset of clinical signs 
PrPSc is widely distributed in the CNS and lymphoid tissues and 2) currently 
used diagnostic methods are sufficient to detect PrPSc prior to the onset of 
clinical signs. The results of this study suggest that there are many 
similarities in the manifestation of CWD and scrapie in white-tailed deer after 
IC inoculation including early and widespread presence of PrPSc in lymphoid 
tissues, clinical signs of depression and weight loss progressing to wasting, 
and an incubation time of 21-23 months. Moreover, western blots (WB) done on 
brain material from the obex region have a molecular profile consistent with CWD 
and distinct from tissues of the cerebrum or the scrapie inoculum. However, 
results of microscopic and IHC examination indicate that there are differences 
between the lesions expected in CWD and those that occur in deer with scrapie: 
amyloid plaques were not noted in any sections of brain examined from these deer 
and the pattern of immunoreactivity by IHC was diffuse rather than plaque-like. 
After a natural route of exposure, 100% of white-tailed deer were susceptible to 
scrapie. Deer developed clinical signs of wasting and mental depression and were 
necropsied from 28 to 33 months PI. Tissues from these deer were positive for 
scrapie by IHC and WB. Tissues with PrPSc immunoreactivity included brain, 
tonsil, retropharyngeal and mesenteric lymph nodes, hemal node, Peyer’s patches, 
and spleen. While two WB patterns have been detected in brain regions of deer 
inoculated by the natural route, unlike the IC inoculated deer, the pattern 
similar to the scrapie inoculum predominates. 
2011 Annual Report 
Research Project: TRANSMISSION, DIFFERENTIATION, AND PATHOBIOLOGY OF 
TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES Location: Virus and Prion Research 
Unit 
2011 Annual Report 
In 
Objective 1, Assess cross-species transmissibility of transmissible spongiform 
encephalopathies (TSEs) in livestock and wildlife, numerous experiments 
assessing the susceptibility of various TSEs in different host species were 
conducted. Most notable is deer inoculated with scrapie, which exhibits 
similarities to chronic wasting disease (CWD) in deer suggestive of sheep 
scrapie as an origin of CWD. 
snip... 
4.Accomplishments 1. Deer inoculated with domestic isolates of sheep 
scrapie. Scrapie-affected deer exhibit 2 different patterns of disease 
associated prion protein. In some regions of the brain the pattern is much like 
that observed for scrapie, while in others it is more like chronic wasting 
disease (CWD), the transmissible spongiform encephalopathy typically associated 
with deer. This work conducted by ARS scientists at the National Animal Disease 
Center, Ames, IA suggests that an interspecies transmission of sheep scrapie to 
deer may have been the origin of CWD. This is important for husbandry practices 
with both captive deer, elk and sheep for farmers and ranchers attempting to 
keep their herds and flocks free of CWD and scrapie. 
White-tailed Deer are Susceptible to Scrapie by Natural Route of 
Infection 
Jodi D. Smith, Justin J. Greenlee, and Robert A. Kunkle; Virus and Prion 
Research Unit, National Animal Disease Center, USDA-ARS 
snip...
This work demonstrates for the first time that white-tailed deer are 
susceptible to sheep scrapie by potential natural routes of inoculation. 
In-depth analysis of tissues will be done to determine similarities between 
scrapie in deer after intracranial and oral/intranasal inoculation and chronic 
wasting disease resulting from similar routes of inoculation. 
see 
full text ; 
SEE 
MORE USAHA REPORTS HERE, 2012 NOT PUBLISHED YET...TSS 
Thursday, June 20, 2013 
atypical, BSE, CWD, Scrapie, Captive Farmed shooting pens (livestock), 
Wild Cervids, Rectal Mucosa Biopsy 2012 USAHA Proceedings, and CJD TSE prion 
Update 
*** 
The potential impact of prion diseases on human health was greatly magnified by 
the recognition that interspecies transfer of BSE to humans by beef ingestion 
resulted in vCJD. While changes in animal feed constituents and slaughter 
practices appear to have curtailed vCJD, there is concern that CWD of 
free-ranging deer and elk in the U.S. might also cross the species barrier. 
Thus, consuming venison could be a source of human prion disease. Whether BSE 
and CWD represent interspecies scrapie transfer or are newly arisen prion 
diseases is unknown. Therefore, the possibility of transmission of prion disease 
through other food animals cannot be ruled out. There is evidence that vCJD can 
be transmitted through blood transfusion. There is likely a pool of unknown size 
of asymptomatic individuals infected with vCJD, and there may be asymptomatic 
individuals infected with the CWD equivalent. These circumstances represent a 
potential threat to blood, blood products, and plasma supplies. 
Thursday, August 08, 2013 
Characterization of the first case of naturally occurring chronic 
wasting disease in a captive red deer (Cervus elaphus) in North America
Friday, August 09, 2013 
CWD 
TSE prion, plants, vegetables, and the potential for environmental 
contamination
Friday, February 08, 2013 
*** 
Behavior of Prions in the Environment: Implications for Prion Biology 
Friday, December 14, 2012 
DEFRA U.K. What is the risk of Chronic Wasting Disease CWD being 
introduced into Great Britain? A Qualitative Risk Assessment October 2012 
Therefore, it is considered that farmed and park deer may have a higher 
probability of exposure to CWD transferred to the environment than wild deer 
given the restricted habitat range and higher frequency of contact with tourists 
and returning GB residents. 
snip... 
In 
the USA, under the Food and Drug Administration’s BSE Feed Regulation (21 CFR 
589.2000) most material (exceptions include milk, tallow, and gelatin) from deer 
and elk is prohibited for use in feed for ruminant animals. With regards to feed 
for non-ruminant animals, under FDA law, CWD positive deer may not be used for 
any animal feed or feed ingredients. For elk and deer considered at high risk 
for CWD, the FDA recommends that these animals do not enter the animal feed 
system. However, this recommendation is guidance and not a requirement by law. 
Animals considered at high risk for CWD include: 
1) 
animals from areas declared to be endemic for CWD and/or to be CWD eradication 
zones and 
2) 
deer and elk that at some time during the 60-month period prior to slaughter 
were in a captive herd that contained a CWD-positive animal. 
Therefore, in the USA, materials from cervids other than CWD positive 
animals may be used in animal feed and feed ingredients for non-ruminants. 
The 
amount of animal PAP that is of deer and/or elk origin imported from the USA to 
GB can not be determined, however, as it is not specified in TRACES. It may 
constitute a small percentage of the 8412 kilos of non-fish origin processed 
animal proteins that were imported from US into GB in 2011. 
Overall, therefore, it is considered there is a __greater than 
negligible risk___ that (nonruminant) animal feed and pet food containing deer 
and/or elk protein is imported into GB. 
There is uncertainty associated with this estimate given the lack of 
data on the amount of deer and/or elk protein possibly being imported in these 
products. 
snip... 
SNIP...SEE ; 
Friday, December 14, 2012 
DEFRA U.K. What is the risk of Chronic Wasting Disease CWD being 
introduced into Great Britain? A Qualitative Risk Assessment October 2012 
Saturday, February 04, 2012 
Wisconsin 16 MONTH age limit on testing dead deer Game Farm CWD Testing 
Protocol Needs To Be Revised 
Monday, June 24, 2013 
The 
Effects of Chronic Wasting Disease on the Pennsylvania Cervid Industry Following 
its Discovery 
Thursday, July 11, 2013 
The 
New Hornographers: The Fight Over the Future of Texas Deer, Captive shooting 
pens, and the CWD TSE prion disease 
Tuesday, July 02, 2013 
National Rifle Association and the Unified Sportsman of Florida support 
a Florida ban on the importation of captive deer and cervids into Florida 
Sunday, June 09, 2013 
Missouri House forms 13-member Interim Committee on the Cause and Spread 
of Chronic Wasting Disease CWD 
Tuesday, April 16, 2013 
Cervid Industry Unites To Set Direction for CWD Reform and seem to 
ignore their ignorance and denial in their role in spreading Chronic Wasting 
Disease 
Tuesday, February 28, 2012 
newly developed injectable CWD vaccine, live rectal mucosa testing and Deer Game Farms Update
http://chronic-wasting-disease.blogspot.com/2012/02/newly-developed-injectable-cwd-vaccine.html
newly developed injectable CWD vaccine, live rectal mucosa testing and Deer Game Farms Update
http://chronic-wasting-disease.blogspot.com/2012/02/newly-developed-injectable-cwd-vaccine.html
AD.24: Development of an oral vaccine for chronic wasting disease 
Ryan Taschuk1,3 Kristen Marciniuk,1,2 Suresh Tikoo,1,3 Philip Griebel,1 
Andrew Potter,1 Neil Cashman5 and Scott Napper1,2
1University of Saskatchewan; VIDO-lnterVac; Saskatoon, SK Canada; 
2Department of Biochemistry; University of Saskatchewan; Saskatoon, SK Canada; 
3School of Public Health; University of Saskatchewan; Saskatoon, SK Canada; 
4Brain Research Centre; University of British Columbia; Vancouver, BC Canada; 
5Brain Research Center; University of British Columbia; Vancouver, BC Canada 
The 
prion protein is well conserved across mammals, and the misfolded protein is the 
causative agent in many animal-specific prion diseases, including chronic 
wasting disease (CWD) in deer and elk. Prion diseases are caused by misfolding 
of endogenously expressed prion protein from the native and homeostatic Prpc 
conformation to the infectious and pathogenic PrPsc conformation. Transmissible 
spongiform encephalopathies are of great interest for many reasons: the onset of 
disease inevitably leads to neurodegeneration and death, the potential of 
interference with food production through transmission both within and between 
agricultural species can have severe economic impacts, and the potential exists 
for zoonotic transmission. Our group has hypothesized that immunotherapeutic 
targeting of the PrPSc conformation would clear the infectious agent / infected 
cell while sparing native PrP, and vaccines may have potential application in 
prevention of CWD transmission or therapeutic treatment of disease. 
Our 
research has focused upon identifying and optimizing three components of a 
potential CWD vaccine: a CWD-disease specific epitope (DSE) that induces 
antibody responses, a carrier protein to increase the magnitude and duration of 
antibody responses toward DSEs, and identification of delivery systems for oral 
delivery of the above DSE-carrier protein ro cervids. We have developed and 
optimized DSEs from three distinct regions of PrPc. Vaccination trials using 
iterations of these DSEs elicit high titers of epitope-specific serum antibody. 
A second generation carrier protein has increased both the duration and 
magnitude of antibody responses when compared with our previous carrier protein. 
Lastly, two delivery systems were effective in inducing antibody responses when 
administered orally to white-tailed deer. We have identified the vaccine 
components necessary for delivering a CWD vaccine to wild cervids. These 
findings will direct our final CWD vaccine formulation and delivery system. 
Sunday, August 11, 2013 
Development of an oral vaccine for chronic wasting disease 
Sunday, July 21, 2013 
*** 
As Chronic Wasting Disease CWD rises in deer herd, what about risk for humans? 
kind regards,
terry


